| 摘要 | 第12-14页 |
| Abstract | 第14-15页 |
| 1 Review of literature and objectives | 第16-39页 |
| 1.1 RNA interference (RNAi) | 第16-17页 |
| 1.2 Classes of RNAi | 第17-25页 |
| 1.2.1 Small RNAs | 第17-19页 |
| 1.2.2 Core components of RNAi | 第19-25页 |
| 1.3 Ago proteins | 第25-34页 |
| 1.3.1 Ago1 protein | 第29-31页 |
| 1.3.2 Ago2 protein | 第31-33页 |
| 1.3.3 Ago3 protein | 第33-34页 |
| 1.4 RNAi process in insects | 第34-38页 |
| 1.4.1 RNAi process in Lepidopteran insects | 第35-37页 |
| 1.4.2 RNAi in Plutella xylostella | 第37-38页 |
| 1.5 Objectives | 第38-39页 |
| 2 Molecular cloning and functional characterization of Argonaute2 in RNAinterference pathway of Plutella xylostella | 第39-67页 |
| 2.1 Introduction | 第39-40页 |
| 2.2 Materials and methods | 第40-54页 |
| 2.2.1 Materials | 第40-41页 |
| 2.2.2 Methods | 第41-54页 |
| 2.3 Results | 第54-65页 |
| 2.3.1 Identificaiton and characterization of the deduced amino acid sequence ofPxAgo2 | 第54-58页 |
| 2.3.3 PxAgo2 mRNA and protein expression level in P. xylostella | 第58-59页 |
| 2.3.4 dsRNA-mediated suppression of PxAgo2 in P. xylsotella larvae | 第59-61页 |
| 2.3.5 PxAgo2 knockdown highly reduces the RNAi efficiency in P. xylostella cells | 第61-63页 |
| 2.3.6 PxAgo2 overexpression enhanced the RNAi efficiency in P. xylostellacells | 第63-65页 |
| 2.4 Discussion | 第65-67页 |
| 3 Molecular characterization and function of Argonaute3 RNA interferencepathway of Plutella xylostella | 第67-84页 |
| 3.1 Introduction | 第67-68页 |
| 3.2 Materials and methods | 第68-71页 |
| 3.2.1 Molecular cloning and expression vector construction of PxAgo3 | 第68-69页 |
| 3.2.2 Phylogenetic tree and sequence analysis | 第69-71页 |
| 3.2.3 dsRNA synthesis and injection into P. xylostella larvae | 第71页 |
| 3.3 Results | 第71-82页 |
| 3.3.1 Identification and sequence characterization of PxAgo3 | 第71-77页 |
| 3.3.2 PxAgo3 expression in response to dsRNA in P. xylostella larvae | 第77页 |
| 3.3.3 PxAgo3 suppression decreased the RNAi efficiency in P. xylostella cells | 第77-79页 |
| 3.3.4 PxAgo3 overexpression increased the RNAi efficiency in P. xylostella cells | 第79-82页 |
| 3.4 Discussion | 第82-84页 |
| 4 Discussion, conclusion and future perspectives | 第84-88页 |
| 4.1 Discussion and conclusion | 第84-87页 |
| 4.2 Future perspectives | 第87-88页 |
| References | 第88-111页 |
| Acknowledgements | 第111-113页 |
| Appedix | 第113-121页 |
| Published Papers and other Academic Achievements duringthe Ph.D. Program | 第121页 |
