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Morphological, Biochemical and Genetic Variability of Snakehead Species Based on Morphometric and Microsatellite Markers

Abstract第8-10页
摘要第11-13页
Abbrevation第13-16页
Chapter 1 Review of literature第16-40页
    1.1. Introduction of the snakeheads第16-25页
        1.1.1. Importance of the snakeheads in aquaculture第16-17页
        1.1.2. General biology of the snakeheads第17页
            1.1.2.1. Ecology第17页
            1.1.2.2. Feeding第17页
            1.1.2.3. Reproduction第17页
        1.1.3. Morphological taxonomy of the snakeheads第17-18页
        1.1.4. Introduction to morphometrics第18-25页
            1.1.4.1 Traditional Morphometrics第19-20页
            1.1.4.2 Truss network measurements第20-22页
            1.1.4.3. Principal Component Analysis (PCA)第22-23页
            1.1.4.4. Discriminant Function Analysis (DFA)第23页
            1.1.4.5. Studies based on the truss network第23-25页
    1.2 Blood functions第25-29页
        1.2.1. Fish blood第25-26页
        1.2.2. Haematological adaptations第26页
        1.2.3. Multiple components第26-27页
        1.2.4. Seasonal effect on hematology第27页
        1.2.5. Effects of low temperature and fasting on hematology第27-28页
        1.2.6. Effects of toxicity on hematology and serum chemistry第28页
        1.2.7. Effects of stress on hematology第28-29页
    1.3. Microsatellites第29-33页
        1.3.1. Methodology for the detection of microsatellites第30页
        1.3.2. The development of microsatellite markers第30-31页
        1.3.3. The efficiency of microsatellite marker第31-32页
        1.3.4. Properties of microsatellite marker第32页
        1.3.5. Constraints of using microsatellite marker第32-33页
    1.4. Conservation of genetic diversity第33-38页
        1.4.1. Molecular markers第35页
        1.4.2. Genetic diversity of native fish populations第35-37页
        1.4.3. Genetic diversity of captive fish lots第37-38页
    1.5. Objectives of the study第38-40页
Chapter 2 Morphometric and meristic characterization of northern snakehead Channa argus(Cantor 1842) blotched snakehead Channa maculata (Lacepde,1801) and theirhybrid(Channa argus(?)×Channa maculata♀)第40-53页
    2.1. Introduction第40-42页
    2.2. Material and method第42-45页
        2.2.1 Sampling method第42-44页
        2.2.2 Statistical analysis第44-45页
    2.3. Results第45-51页
        2.3.1. Meristic traits第45-47页
        2.3.2. Morphometric traits第47-51页
    2.4. Discussion第51-53页
Chapter 3 Haematological and serum biochemical characterization and comparison betweenwild and cultured Northern snakehead (Channa argus Cantor,1842)第53-68页
    3.1. Introduction第53-54页
    3.2. Material and method第54-59页
        3.2.1. Water quality analysis第54-55页
        3.2.2. Collection of fishes and blood sample第55-56页
        3.2.3. Laboratory analysis of blood sample第56-58页
            3.2.3.1. Heamoglobin content第56页
            3.2.3.2. Haematocrit第56页
            3.2.3.3. Erythrocyte sedimentation rate第56-57页
            3.2.3.4. General cytological and cytochemical staining of blood films第57页
            3.2.3.5. Total erythrocyte and leukocyte counts第57页
            3.2.3.6. Corpuscular indices第57-58页
            3.2.3.7. Size measurement of erythrocyte and leukocyte第58页
        3.2.4. Clinical analysis第58-59页
    3.3. Results第59-65页
        3.3.1. Water quality analysis第59-60页
        3.3.2. Haemotological characterization of wild and cultured northern snakehead第60-62页
        3.3.3. Serum biochemical characterization of wild and cultured northern snakehead第62-65页
    3.4 Discussion第65-68页
Chapter 4 The isolation and characterization of 10 dinucleotide microsatellite markers fromenriched Channa argus genomic library第68-76页
    4.1. Introduction第68-69页
    4.2. Material and method第69-73页
        4.2.1. Microsatellite-enriched library construction第69-71页
        4.2.2. Sequence analysis and primer design第71-72页
        4.2.3. DNA extraction, PCR amplification第72-73页
        4.2.4 Genetic analysis第73页
    4.3. Results and discussion第73-76页
        4.3.1. Genomic library and SSR markers development第73-76页
Chapter 5 Genetic variation of wild and hatchery populations of the northern snakeheadChanna argus assessed by Microsatellite markers第76-89页
    5.1. Introduction第76-77页
    5.2. Materials and methods第77-80页
        5.2.1. Sample collection and DNA extraction第77-79页
        5.2.2. Microsatellite analysis第79-80页
        5.2.3. Data Analyses第80页
            5.2.3.1. Genetic diversity第80页
            5.2.3.2. Population Structure第80页
    5.3. Results第80-87页
        5.3.1. Genetic variability among population第80-81页
        5.3.2. Population genetic structure第81-85页
        5.3.3. Deviation from Hardy-Weinberg expectation第85页
        5.3.4. Genetic distance第85-86页
        5.3.5. Cluster analysis and UPGMA dandrogram第86-87页
    5.5. Discussion第87-89页
Chapter 6 Major findings and future recommendations第89-90页
References第90-119页
Curriculum vitae(C.V.)第119-123页
Acknowledgements第123-124页
Appendix第124-125页

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