| 摘要 | 第13-15页 |
| ABSTRACT | 第15-16页 |
| CHAPTER I LITERATURE REVIEW | 第17-43页 |
| 1.1 Heavy metals in soils and plants | 第17-25页 |
| 1.1.1 Contaminated heavy metal soil | 第17-19页 |
| 1.1.2 Cadmium in the soil-plant relationship | 第19-22页 |
| 1.1.3 Mechanism of uptake heavy metal into plant | 第22-25页 |
| 1.2 Phytoremediation | 第25-31页 |
| 1.2.1 Detoxification mechanisms in plants | 第25-27页 |
| 1.2.2 Using plant for phytoremediation | 第27-31页 |
| 1.3 Introduction about sorghum | 第31-36页 |
| 1.3.1 Sweet sorghum and application value | 第31-33页 |
| 1.3.2 Respones of sweet sorghum to heavy metal | 第33-36页 |
| 1.4 Principles of transcriptome analysis and gene expression quantification | 第36-43页 |
| 1.4.1 m RNA seq- transcriptome | 第36-38页 |
| 1.4.2 Identification of gene expression of plant responses to adverseenvironmental condition | 第38-43页 |
| CHAPTER II PHOTOSYNTHESIS AND GROWTH RESPONSE OF SWEET SORGHUM PLANTSTO DIFFERENT LEVELS OF HEAVY METAL CADMIUM | 第43-61页 |
| 2.1 Introduction | 第43-44页 |
| 2.2 Material and methods | 第44-46页 |
| 2.2.1 Plant materials and growth conditions | 第44-45页 |
| 2.2.2 Growth characteristic analysis | 第45页 |
| 2.2.3 Photosynthesis analysis | 第45-46页 |
| 2.2.4 Data analysis | 第46页 |
| 2.3 Results | 第46-56页 |
| 2.3.1 Growth characteristic | 第46-50页 |
| 2.3.2 Effect of Cd2+ on plant photosynthesis parameters | 第50-56页 |
| 2.4 Discussion | 第56-59页 |
| 2.5 Conclusions | 第59-61页 |
| CHAPTER III CADMIUM ACCUMULATION IN PLANT AND EFFECT ON NUTRIENTELEMENTS | 第61-85页 |
| 3.1 Introduction | 第61-63页 |
| 3.2 Material and methods | 第63-66页 |
| 3.2.1 Plant materials and growth conditions | 第63-64页 |
| 3.2.2 Sampling methods | 第64-65页 |
| 3.2.3 Measurement of metal concentration | 第65-66页 |
| 3.2.4 Data analysis | 第66页 |
| 3.3 Result | 第66-80页 |
| 3.3.1 Cd concentration in organs of sweet sorghum at the seedling stage .. 50 | 第66-68页 |
| 3.3.2 Cd concentration in organs of sweet sorghum at the hard dough stage | 第68-75页 |
| 3.3.3 Effects of cadmium on uptake and translocation of nutrient elements | 第75-80页 |
| 3.4 Discussion | 第80-82页 |
| 3.4.1 Distribution of Cd in sweet sorghum | 第80-81页 |
| 3.4.2 Effect of Cd on nutrient elements transport in plant | 第81-82页 |
| 3.5 Conclusions | 第82-85页 |
| CHAPTER IV CADMIUM INDUCED MODIFICATION OF SUGAR ACCUMULATION ANDTRANSLOCATION ALONG SWEET SORGHUM STEM | 第85-97页 |
| 4.1 Introduction | 第85-87页 |
| 4.2 Material and methods | 第87-89页 |
| 4.2.1 Plant materials and growth conditions | 第87-88页 |
| 4.2.2 Measure sugar content | 第88-89页 |
| 4.2.3 Data analysis | 第89页 |
| 4.3 Results | 第89-92页 |
| 4.3.1 Cd effect on total soluble solid in stem of sweet sorghum | 第89-90页 |
| 4.3.2 The relationship between Cd concentration and and Brix | 第90-91页 |
| 4.3.3 Cd effect on Soluble Sugar in stem of sweet sorghum | 第91-92页 |
| 4.4 Discussion | 第92-95页 |
| 4.4.1 Cd effect on Brix | 第92-93页 |
| 4.4.2 Cd effect on soluble sugar | 第93-95页 |
| 4.5 Conclusions | 第95-97页 |
| CHAPTER V TRANSCRIPTOME ANALYSIS OF SWEET SORGHUM IN RESPONSE TOCADMIUM STRESSES | 第97-125页 |
| 5.1 Introduction | 第97-99页 |
| 5.2 Material and methods | 第99-103页 |
| 5.2.1 Plant Growth Conditions | 第99-100页 |
| 5.2.2 Harvesting plant material | 第100页 |
| 5.2.3 Sequencing Protocol | 第100-103页 |
| 5.3 Results | 第103-124页 |
| 5.3.1 Isolation of Total RNA results | 第103页 |
| 5.3.2 Illumina Sequencing and the Assembly of Sequence Reads | 第103-104页 |
| 5.3.3 Differential Expression Analysis | 第104-106页 |
| 5.3.4 Gene Ontology (GO) Functional Enrichment Analysis for the DEGs | 第106-110页 |
| 5.3.5 KEGG pathway enrichment analysis of differential gene | 第110-113页 |
| 5.3.6 Functional analyses of differentially expressed genes | 第113-124页 |
| 5.4 Conclusions | 第124-125页 |
| CONCLUSSION AND OUTLOOK | 第125-129页 |
| REFERENCES | 第129-147页 |
| Acknowledgements | 第147-149页 |
| RESEARCH ARTICLES PUBLISHED | 第149页 |
