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葡萄品种资源灰霉病抗性鉴定及抗病与感病寄主抗氧化酶、活性氧和茉莉酸分析

摘要第7-9页
ABSTRACT第9-10页
CHAPTER1 LITERATURE REVIEW第14-20页
    1.1 Grapevines第14页
    1.2 Botrytis cinerea第14-15页
    1.3 Anti-oxidant Enzymes第15-16页
    1.4 Reactive Oxygen Species第16-18页
    1.5 Jasmonic Acid第18-20页
CHAPTER2 Screening Vitis Genotypes for Responses to Botrytis cinerea and Evaluation of Antioxidant Enzymes,Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts第20-39页
    2.1 Objectives第20页
    2.2 Materials and Methods第20-23页
        2.2.1 Plant and Fungal Materials第20-21页
        2.2.2 Detached Leaf Evaluation第21页
        2.2.3 Disease Severity Rating第21页
        2.2.4 Light Microscopy第21-22页
        2.2.5 Scanning Electron Microscope(SEM)第22页
        2.2.6 ROS Measurement第22-23页
        2.2.7 Enzyme Extraction and Activity Assays第23页
        2.2.8 JA Quantification in HR and HS Genotypes第23页
        2.2.9 Statistical Analysis第23页
    2.3 RESULTS第23-36页
        2.3.1.Grape Genotypes Exhibit Different Levels of Resistance to B.cinerea第23-31页
        2.3.2.B.cinerea Growth on the HR Genotype’Zi Qiu’and the HS Genotype‘Riesling’第31-33页
        2.3.3.Activity of Peroxidase and Superoxide Dismutase in the HR genotype’Zi Qiu’and the HS Genotype‘Riesling’Infected by B.cinerea第33-34页
        2.3.4.Hydrogen Peroxide(H2O2)Accumulation in HR’Zi Qiu’and HS‘Riesling’Leaves in Response to Infection with B.cinerea第34页
        2.3.5.Accumulation of Superoxide Radicals(O2? )in HR’Zi Qiu’and HS‘Riesling’Leaves in Response to Infection with B.cinerea第34-35页
        2.3.6.Jasmonic Acid Levels in Leaves of the HR genotype‘Zi Qiu’and the HS Genotype‘Riesling’Following Inoculation with B.cinerea第35页
        2.3.7.Pearson’s Correlation Coefficients第35-36页
    2.4.Discussion第36-38页
    2.5.Conclusions第38-39页
CHAPTER3 In vitro evaluation of berries of various Vitis genotypes for disease resistance to Botrytis cinerea第39-53页
    3.1.Objectives第39页
    3.2.Materials and Methods第39-41页
        3.2.1 Grape berries and fungal materials:第39页
        3.2.2 Berry selection and inoculation:第39页
        3.2.3 Disease severity rating:第39-40页
        3.2.4 Light microscopy:第40页
        3.2.5 Reactive oxygen species measurement:第40页
        3.2.6 Enzyme extraction and activity assay:第40-41页
        3.2.7 JA quantification:第41页
        3.2.8 Statistical analysis:第41页
    3.3 Results第41-48页
        3.3.1 O_2~? and H_2O_2 Accumulation in the Interactions of B.cinerea with HR Dong fang zhi xing and HS Gold finger:第47页
        3.3.2 JA Levels in HR“Dong fang zhi xing”and HS“Gold finger”in Interaction with B.cinerea:第47-48页
    3.4 Discussion第48-52页
        3.4.1 Vitis genotypes Exhibited Different Levels of Resistance to B.cinerea:第48-49页
        3.4.2 Activities of Malondialhydrate(MDA),Superoxide and Dismutase Peroxidase in HR Dong fang zhi xing and HS Gold finger Infected by B.cinerea第49-50页
        3.4.3 O_2~?and H_2O_2 Accumulation in the Interactions of B.cinerea with HR Dong fang zhi xing and HS Gold finger第50-51页
        3.4.4 JA Levels in HR“Dong fang zhi xing”and HS“Gold finger”in Interaction with B.cinerea第51-52页
    3.5 Conclusions第52-53页
CHAPTER4 Botrytis cinerea structural development and host responses in resistant and susceptible grapevine cultivars:A physiological and structural study第53-64页
    4.1 Objectives:第53页
    4.2 Materials and Methods:第53-55页
        4.2.1 Plant and Fungal Material第53页
        4.2.2 Disease incidence(%)第53-54页
        4.2.3 Light Microscopy第54页
        4.2.4 Scanning Electron Microscopy(SEM)第54页
        4.2.5 Reactive Oxygen Species Measurement第54-55页
        4.2.6 Statistical Analysis第55页
    4.3 Results第55-61页
        4.3.1.B.cinerea Growth on the Marselan and Blue French第55-58页
        4.3.2 Activities of Peroxidase(POD),Superoxide Dismutase(SOD)and Catalase(CAT)in Marselan and Blue French cultivars Infected with B.cinerea第58-60页
        4.3.3 H_2O_2 Accumulation in B.cinerea inoculated cultivars Marselan and Blue French第60页
        4.3.4.O_2~? Accumulation in the Interactions of B.cinerea with Marselan and Blue French第60-61页
    4.4 Discussion第61-64页
Chapter5 Conclusions and Recommendations第64-65页
References第65-72页
Abbreviations第72-73页
Acknowledgement第73-74页
About Author第74页

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