| 摘要 | 第7-9页 |
| ABSTRACT | 第9-10页 |
| CHAPTER1 LITERATURE REVIEW | 第14-20页 |
| 1.1 Grapevines | 第14页 |
| 1.2 Botrytis cinerea | 第14-15页 |
| 1.3 Anti-oxidant Enzymes | 第15-16页 |
| 1.4 Reactive Oxygen Species | 第16-18页 |
| 1.5 Jasmonic Acid | 第18-20页 |
| CHAPTER2 Screening Vitis Genotypes for Responses to Botrytis cinerea and Evaluation of Antioxidant Enzymes,Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts | 第20-39页 |
| 2.1 Objectives | 第20页 |
| 2.2 Materials and Methods | 第20-23页 |
| 2.2.1 Plant and Fungal Materials | 第20-21页 |
| 2.2.2 Detached Leaf Evaluation | 第21页 |
| 2.2.3 Disease Severity Rating | 第21页 |
| 2.2.4 Light Microscopy | 第21-22页 |
| 2.2.5 Scanning Electron Microscope(SEM) | 第22页 |
| 2.2.6 ROS Measurement | 第22-23页 |
| 2.2.7 Enzyme Extraction and Activity Assays | 第23页 |
| 2.2.8 JA Quantification in HR and HS Genotypes | 第23页 |
| 2.2.9 Statistical Analysis | 第23页 |
| 2.3 RESULTS | 第23-36页 |
| 2.3.1.Grape Genotypes Exhibit Different Levels of Resistance to B.cinerea | 第23-31页 |
| 2.3.2.B.cinerea Growth on the HR Genotype’Zi Qiu’and the HS Genotype‘Riesling’ | 第31-33页 |
| 2.3.3.Activity of Peroxidase and Superoxide Dismutase in the HR genotype’Zi Qiu’and the HS Genotype‘Riesling’Infected by B.cinerea | 第33-34页 |
| 2.3.4.Hydrogen Peroxide(H2O2)Accumulation in HR’Zi Qiu’and HS‘Riesling’Leaves in Response to Infection with B.cinerea | 第34页 |
| 2.3.5.Accumulation of Superoxide Radicals(O2? )in HR’Zi Qiu’and HS‘Riesling’Leaves in Response to Infection with B.cinerea | 第34-35页 |
| 2.3.6.Jasmonic Acid Levels in Leaves of the HR genotype‘Zi Qiu’and the HS Genotype‘Riesling’Following Inoculation with B.cinerea | 第35页 |
| 2.3.7.Pearson’s Correlation Coefficients | 第35-36页 |
| 2.4.Discussion | 第36-38页 |
| 2.5.Conclusions | 第38-39页 |
| CHAPTER3 In vitro evaluation of berries of various Vitis genotypes for disease resistance to Botrytis cinerea | 第39-53页 |
| 3.1.Objectives | 第39页 |
| 3.2.Materials and Methods | 第39-41页 |
| 3.2.1 Grape berries and fungal materials: | 第39页 |
| 3.2.2 Berry selection and inoculation: | 第39页 |
| 3.2.3 Disease severity rating: | 第39-40页 |
| 3.2.4 Light microscopy: | 第40页 |
| 3.2.5 Reactive oxygen species measurement: | 第40页 |
| 3.2.6 Enzyme extraction and activity assay: | 第40-41页 |
| 3.2.7 JA quantification: | 第41页 |
| 3.2.8 Statistical analysis: | 第41页 |
| 3.3 Results | 第41-48页 |
| 3.3.1 O_2~? and H_2O_2 Accumulation in the Interactions of B.cinerea with HR Dong fang zhi xing and HS Gold finger: | 第47页 |
| 3.3.2 JA Levels in HR“Dong fang zhi xing”and HS“Gold finger”in Interaction with B.cinerea: | 第47-48页 |
| 3.4 Discussion | 第48-52页 |
| 3.4.1 Vitis genotypes Exhibited Different Levels of Resistance to B.cinerea: | 第48-49页 |
| 3.4.2 Activities of Malondialhydrate(MDA),Superoxide and Dismutase Peroxidase in HR Dong fang zhi xing and HS Gold finger Infected by B.cinerea | 第49-50页 |
| 3.4.3 O_2~?and H_2O_2 Accumulation in the Interactions of B.cinerea with HR Dong fang zhi xing and HS Gold finger | 第50-51页 |
| 3.4.4 JA Levels in HR“Dong fang zhi xing”and HS“Gold finger”in Interaction with B.cinerea | 第51-52页 |
| 3.5 Conclusions | 第52-53页 |
| CHAPTER4 Botrytis cinerea structural development and host responses in resistant and susceptible grapevine cultivars:A physiological and structural study | 第53-64页 |
| 4.1 Objectives: | 第53页 |
| 4.2 Materials and Methods: | 第53-55页 |
| 4.2.1 Plant and Fungal Material | 第53页 |
| 4.2.2 Disease incidence(%) | 第53-54页 |
| 4.2.3 Light Microscopy | 第54页 |
| 4.2.4 Scanning Electron Microscopy(SEM) | 第54页 |
| 4.2.5 Reactive Oxygen Species Measurement | 第54-55页 |
| 4.2.6 Statistical Analysis | 第55页 |
| 4.3 Results | 第55-61页 |
| 4.3.1.B.cinerea Growth on the Marselan and Blue French | 第55-58页 |
| 4.3.2 Activities of Peroxidase(POD),Superoxide Dismutase(SOD)and Catalase(CAT)in Marselan and Blue French cultivars Infected with B.cinerea | 第58-60页 |
| 4.3.3 H_2O_2 Accumulation in B.cinerea inoculated cultivars Marselan and Blue French | 第60页 |
| 4.3.4.O_2~? Accumulation in the Interactions of B.cinerea with Marselan and Blue French | 第60-61页 |
| 4.4 Discussion | 第61-64页 |
| Chapter5 Conclusions and Recommendations | 第64-65页 |
| References | 第65-72页 |
| Abbreviations | 第72-73页 |
| Acknowledgement | 第73-74页 |
| About Author | 第74页 |
