| 摘要 | 第4-12页 |
| Abstract | 第12-14页 |
| Chapter Ⅰ Review of the factors that affect cardiac performance and the role of relevant genes | 第19-47页 |
| 1.1 Conservation of Drosophila and mammalian CAMRs | 第19-33页 |
| 1.1.1 The role of CAMRs in the nervous system | 第25-27页 |
| 1.1.2 Involvement of CAMRs in the Notch signaling pathways | 第27-29页 |
| 1.1.3 Role of CAMRs in cell viability and piRNA pathway | 第29-30页 |
| 1.1.4 Association between RyRs, TRP/TRPL and CAMRs | 第30-33页 |
| 1.2 Other relevant cardiac related genes | 第33-37页 |
| 1.2.1 The role of PPTA gene in cardiac performance | 第33-34页 |
| 1.2.2 The function of CaMKⅡ gene in cardiac performance | 第34页 |
| 1.2.3 The SERCA gene and cardiac function | 第34-35页 |
| 1.2.4 The role of Lamin C in cardiomyopathy | 第35页 |
| 1.2.5 Integrin and cardiac function | 第35-36页 |
| 1.2.6 The effect of Delta in cardiac function | 第36-37页 |
| 1.3 Effect of Drosophila melanogaster endurance training assays on cardiac performance | 第37-38页 |
| 1.3.1 Effect of exercise training on cardiovascular disease | 第37-38页 |
| 1.3.2 Endurance training assays for Drosophila melanogaster | 第38页 |
| 1.4 Fat and sugar metabolism in mammals and Drosophila | 第38-42页 |
| 1.4.1 Excess fat and sugar in Drosophila causes diabetic cardiomyopathy-like symptoms | 第40页 |
| 1.4.2 Effect of high-dat diet (HFD) on Drosophila heart function | 第40-41页 |
| 1.4.3 Effect of high-sugar diet (HSD) on Drosophila heart function | 第41页 |
| 1.4.4 Induced-obesity | 第41-42页 |
| 1.5 Mild stress | 第42-43页 |
| 1.6 Starvation resistance | 第43-44页 |
| 1.7 The uses of Cordyceps militaris | 第44页 |
| 1.8 The purpose and significance of the study | 第44-47页 |
| Chapter Ⅱ Bioinformatics analysis of calcium and cardiac related proteins | 第47-57页 |
| 2.1 Introduction | 第47-49页 |
| 2.2 Materials and methods | 第49-50页 |
| 2.2.1 Data-mining, protein structure and functional prediction | 第49页 |
| 2.2.2 Analysis of conserved motifs | 第49页 |
| 2.2.3 Construction of the PPI network | 第49-50页 |
| 2.3 Results | 第50-55页 |
| 2.4 Discussion | 第55-56页 |
| 2.5 Conclusion | 第56-57页 |
| Chapter Ⅲ Cordyceps improves cardiac performance and aging in endurance training Drosophila melanogaster | 第57-85页 |
| 3.1 Introduction | 第57-59页 |
| 3.2 Materials and methods | 第59-63页 |
| 3.2.1 Fly maintenance and collection | 第59页 |
| 3.2.2 Drug administration | 第59页 |
| 3.2.3 Mortality assay | 第59-60页 |
| 3.2.4 Lifespan assay for wild type flies | 第60页 |
| 3.2.5 Negative geotaxis assay | 第60页 |
| 3.2.6 Larval crawling assay | 第60-61页 |
| 3.2.7 Cold stress | 第61页 |
| 3.2.8 RNA extractions and RT-PCR | 第61页 |
| 3.2.9 qRT-PCR | 第61-62页 |
| 3.2.10 Total protein measurement | 第62页 |
| 3.2.11 Western blot analysis | 第62-63页 |
| 3.2.12 Courtship and mating | 第63页 |
| 3.2.13 Statistical analysis | 第63页 |
| 3.3 Results | 第63-80页 |
| 3.3.1 The role of Cordyceps and exercise in aging and cardiac function | 第63-68页 |
| 3.3.2 Effect of exercise and Cordyceps on Gene and protein expression | 第68-78页 |
| 3.3.3 Cordyceps does not improve mating and courtship | 第78-80页 |
| 3.4 Discussion | 第80-83页 |
| 3.5 Conclusion | 第83-85页 |
| Chapter Ⅳ The effect of induced obesity and Cordyceps militaris in Drosophila melanogaster cardiac performance and aging | 第85-117页 |
| 4.1 Introduction | 第85-87页 |
| 4.2 Materials and methods | 第87-90页 |
| 4.2.1 Fly rearing and collection | 第87-88页 |
| 4.2.2 HFD and HSD feeding regime | 第88页 |
| 4.2.3 Drug administration | 第88页 |
| 4.2.4 Lifespan assay for different fly strains | 第88页 |
| 4.2.5 Negative geotaxis assay for different fly strains | 第88页 |
| 4.2.6 Triglyceride, protein and glucose measurements | 第88-89页 |
| 4.2.7 Cold stress | 第89页 |
| 4.2.8 RNA extractions and RT-PCR | 第89页 |
| 4.2.9 qRT-PCR | 第89-90页 |
| 4.2.10 Western blot analysis | 第90页 |
| 4.2.11 Statistical analysis | 第90页 |
| 4.3 Results | 第90-112页 |
| 4.3.1 The effect of Cordyceps militaris in longevity and cardiac function of mutant flies | 第90-91页 |
| 4.3.2 Phenotypic profiles of flies fed with high-fat and high-sugar diet | 第91-99页 |
| 4.3.3 Effect of lipogenic diet on gene and protein expression | 第99-112页 |
| 4.4 Discussion | 第112-115页 |
| 4.5 Conclusion | 第115-117页 |
| Chapter Ⅴ Effect of combined stresses on Drosophila cardiac performance and mobility | 第117-135页 |
| 5.1 Introduction | 第117-120页 |
| 5.2 Materials and methods | 第120-123页 |
| 5.2.1 Fly rearing and collection | 第120页 |
| 5.2.2 Starvation resistance assay | 第120页 |
| 5.2.3 Negative geotaxis assay | 第120-121页 |
| 5.2.4 Cold pretreatment | 第121页 |
| 5.2.5 Cold stress | 第121页 |
| 5.2.6 Triglyceride, protein and glucose measurements | 第121-122页 |
| 5.2.7 RNA extractions and RT-PCR | 第122页 |
| 5.2.8 qRT-PCR | 第122页 |
| 5.2.9 Western blot analysis | 第122-123页 |
| 5.2.10 Statistical analysis | 第123页 |
| 5.3 Results | 第123-131页 |
| 5.3.1 The effect of mild stresses in cardiac function of flies | 第123-127页 |
| 5.3.2 The effect of starvation resistance in the expression of cardiac related genes | 第127-131页 |
| 5.4 Discussion | 第131-134页 |
| 5.5 Conclusion | 第134-135页 |
| Chapter Ⅵ Summary and Conclusion | 第135-139页 |
| References | 第139-155页 |
| Appendices | 第155-157页 |
| Acknowledgement | 第157-159页 |
| Published articles | 第159页 |
| Scholarship Awards | 第159页 |
