| 中文摘要 | 第5-7页 |
| Abstract | 第7-8页 |
| Abbreviations | 第12-16页 |
| CHAPTER-I Literature review | 第16-34页 |
| 1.1 Occurrence of Southern rice black-streaked dwarf disease and damage | 第16页 |
| 1.2 Disease Symptom and alternate hosts | 第16-18页 |
| 1.3 Genome and characteristics of SRBSDV | 第18-20页 |
| 1.4 Economic importance of plant virus diseases caused by plant hoppers | 第20-21页 |
| 1.5 The vector of SRBSDV: Sogatella furcifera (WBPH) | 第21-23页 |
| 1.5.1 Biology and Characteristic of WBPH | 第21-22页 |
| 1.5.2 Distribution of WBPH | 第22页 |
| 1.5.3 WBPH’s life cycle | 第22页 |
| 1.5.4 WBPH-SRBSDV-Host plant interaction | 第22-23页 |
| 1.6 Transmission of plant viruses by Insect vector | 第23-25页 |
| 1.7 Protein-protein interactions | 第25-32页 |
| 1.7.1 Yeast two-hybrid system | 第26-27页 |
| 1.7.2 The Split-ubiquitin yeast two-hybrid system | 第27-29页 |
| 1.7.3 The DUALhunter system | 第29-31页 |
| 1.7.4 GST Pull-down Assay | 第31-32页 |
| 1.8 Principles of Rapid Amplification of c DNA Ends (RACE) | 第32-33页 |
| 1.9 Objectives | 第33-34页 |
| CHAPTER-II Yeast two- hybrid Screening of SRBSDV-P10 interacting proteins in the WBPH | 第34-58页 |
| 2.1 Introduction | 第34-35页 |
| 2.2 Materials and Methods | 第35-49页 |
| 2.2.1 Viruses isolate maintenance and insect vector rearing | 第35页 |
| 2.2.2 c DNA library of Sogatella furcifera | 第35页 |
| 2.2.3 Bait fusion vector (p DHB1+P10) construction | 第35-41页 |
| 2.2.4 Transformation of p DHB1+P10 bait fusion vector into yeast | 第41-42页 |
| 2.2.5 Verifying expression of bait (p DHB1+P10) by Western blot | 第42-43页 |
| 2.2.6 Functional assay for verification of p DHB1+P10 expression | 第43-44页 |
| 2.2.7 Pilot Screening for optimizing the screening stringency | 第44-46页 |
| 2.2.8 Library transformation and selection of interactors | 第46-48页 |
| 2.2.9 Annotation and gene ontology (GO) classification | 第48-49页 |
| 2.2.10 Confirmation of putative positive interactors | 第49页 |
| 2.2.11 Construction of network diagram of interacted proteins of WBPH | 第49页 |
| 2.3 Results | 第49-57页 |
| 2.3.1 Construction of S. furcifera c DNA library | 第49-50页 |
| 2.3.2 Bait fusion vector (p DHB1+P10) construction | 第50页 |
| 2.3.3 Expression of bait (p DHB1+P10) by Western blot | 第50-51页 |
| 2.3.4 Verification of correct expression of bait fusion (p DHB1+P10) protein by functional assay | 第51-52页 |
| 2.3.5 Pilot Screening for optimizing the screening stringency | 第52-53页 |
| 2.3.6 Selection of interactors by library screening | 第53页 |
| 2.3.7 Annotation and gene ontology (GO) classification | 第53-55页 |
| 2.3.8 Confirmation of positive interactors by Retransformation analysis and β-galactosidase assay | 第55-56页 |
| 2.3.9 Construction of network diagram of interacted proteins of WBPH | 第56-57页 |
| 2.4. Discussion | 第57-58页 |
| CHAPTER-III Validation of the putative P10 interacting proteins through GST Pull-Down Assay | 第58-68页 |
| 3.1 Introduction | 第58页 |
| 3.2 Materials and Methods | 第58-64页 |
| 3.2.1 Full length amplification of selected genes | 第58-61页 |
| 3.2.2 Construction of Bait and Prey Vectors | 第61-62页 |
| 3.2.3 GST Pull-Down Assay | 第62-64页 |
| 3.3 Results | 第64-67页 |
| 3.3.1 Full-length gene amplification | 第64页 |
| 3.3.2 Sequence alignment analysis of each protein | 第64-65页 |
| 3.3.3 Construction of bait and prey fusion vectors | 第65-66页 |
| 3.3.4 GST Pull-Down Assay | 第66-67页 |
| 3.4 Discussion | 第67-68页 |
| CHAPTER-IV Quantitative analysis of the transcriptional levels of the SRBSDV-P10 interactingproteins in WBPH via RT-q PCR | 第68-73页 |
| 4.1 Introduction | 第68页 |
| 4.2 Materials and Methods | 第68-70页 |
| 4.2.1 m RNA expression level by RT-q PCR | 第68-70页 |
| 4.3 Results | 第70-72页 |
| 4.3.1 Gene expression analysis in different growth stages and different organs of Sogatella furcifera | 第70-72页 |
| 4.4 Discussion | 第72-73页 |
| CHAPTER-V Discussion | 第73-78页 |
| CHAPTER-VI Conclusion and Future Perspective | 第78-79页 |
| References | 第79-89页 |
| Acknowledgements | 第89-90页 |
| Author biography | 第90-91页 |
| Appendix | 第91-103页 |
