| 中文摘要 | 第3-6页 |
| ABSTRACT | 第6-9页 |
| 1 INTRODUCTION | 第21-31页 |
| 1.1 SOLAR LIGHT AND ULTRAVIOLET RADIATION SYSTEM | 第21-22页 |
| 1.2 PHOTOAGING | 第22-25页 |
| 1.3 MATRIX METALLOPROTEINASES (MMPS) | 第25-27页 |
| 1.4 ERIODICTYOL | 第27-28页 |
| 1.5 BERBERINE | 第28-29页 |
| 1.6 AIMS AND OBJECTIVES OF THE STUDY | 第29-30页 |
| 1.7 PROPOSED MODEL OF THE STUDY | 第30-31页 |
| 2 MATERIALS AND METHODS | 第31-45页 |
| 2.1 MATERIALS, REAGENTS AND ANTIBODIES | 第31页 |
| 2.2 THE MAJOR EXPERIMENTAL EQUIPMENTS, UTENSILS AND SUPPLIES | 第31-33页 |
| 2.3 THE MAIN LABORATORY REAGENTS AND CONSUMABLES | 第33-34页 |
| 2.4 PREPARATION OF SOME REAGENTS | 第34-35页 |
| 2.5 CELL CULTURES | 第35页 |
| 2.6 UVA source and dosimetry | 第35-36页 |
| 2.7 EFFECT OF UVA ON CELL MORPHOLOGY AND CELL VIABILITY | 第36-37页 |
| 2.8 DETECTION OF ROS | 第37页 |
| 2.9 LDH ASSAY | 第37-38页 |
| 2.10 SUPEROXIDE DISMUTASE ASSAY (SOD) | 第38页 |
| 2.11 ELISA FOR MMP-1 | 第38-39页 |
| 2.12 WESTERN BLOT | 第39页 |
| 2.13 PREPARATION OF VARIOUS BUFFERS AND SAMPLE LOADING | 第39-40页 |
| 2.14 GENE EXPRESSION ANALYSIS | 第40-42页 |
| 2.15 CDNA SYNTHESIS | 第42页 |
| 2.16 SEMI-QUANTITATIVE RT-PCR | 第42-43页 |
| 2.17 QUANTITATIVE REAL-TIME PCR (QRT-PCR) | 第43-44页 |
| 2.18 STATISTICAL ANALYSIS | 第44-45页 |
| 3 RESULTS | 第45-63页 |
| 3.1 CELL VIABILITY AND CELL MORPHOLOGY | 第45-49页 |
| 3.1.1 Effect of UVA on HaCaT cell viability and morphology | 第45-46页 |
| 3.1.2 Effect of Eriodictyol and Berberine on UVA irradiated HaCaT cells | 第46-49页 |
| 3.2 UVA GENERATED OXIDATIVE STRESS | 第49-52页 |
| 3.2.1 ROS and effect of ER | 第49页 |
| 3.2.2 ROS and Effect of BBR | 第49-52页 |
| 3.3 THE LACTATE DEHYDROGENASE (LDH) LEAKAGE ASSAY | 第52-54页 |
| 3.4 UVA INDUCED DEPLETION IN SUPER OXIDE DISMUTASE (SOD) ACTIVITY | 第54-55页 |
| 3.5 EFFECT OF ER AND BBR ON UVA-INDUCED MMP-1 EXPRESSION IN HACAT CELLS | 第55-56页 |
| 3.6 ERIODICTYOL MODULATES UVA-INDUCED INFLAMMATORY MEDIATORS IN HACAT CELLS | 第56-57页 |
| 3.7 THE EFFECTS OF UVA IRRADIATION ON MMP-1, TIMP-1, COL-I, COL-III EXPRESSION INHACAT CELLS | 第57-58页 |
| 3.7.1 Effects of Eriodictyol and Berberine | 第57-58页 |
| 3.8 UVA MEDIATED MAPKINASE PHOSPHORYLATION | 第58-60页 |
| 3.8.1 Eriodictyol modulated UVA-driven MAPK phosphorylation | 第58页 |
| 3.8.2 Berberine modulated UVA-driven MAPK phosphorylation | 第58-60页 |
| 3.9 EFFECT OF ER ON THE MODULATION MMP-1, AND ITSACTIVATING TRANSCRIPTION FACTORS | 第60-62页 |
| 3.10 CONCLUSION | 第62-63页 |
| 4 UVA-RESPONSIVE ZNO@AKBA NANO-PARTICLES MAXIMIZE THE TARGETED DELIVERY OF AKBA MOLECULES AND PROVIDE A GREAT POTENTIAL FOR SKIN PROTECTION | 第63-83页 |
| 4.1 ABSTRACT | 第63-64页 |
| 4.2 INTRODUCTION | 第64-65页 |
| 4.3 MATERIALS AND METHODS | 第65-68页 |
| 4.3.1 Materials | 第65页 |
| 4.3.2 Synthesis of Zn O particles | 第65页 |
| 4.3.3 AKBA loading | 第65-66页 |
| 4.3.4 Characterization of Nano-particles | 第66页 |
| 4.3.5 UV-controlled release behavior | 第66页 |
| 4.3.6 Gene expression analyses | 第66-67页 |
| 4.3.7 Cytotoxicity of AKBA@Zn O | 第67-68页 |
| 4.3.8 Statistical Analysis: | 第68页 |
| 4.4 RESULTS & DISCUSSION | 第68-81页 |
| 4.4.1 Preparation and characterization of Zn O particles and AKBA@Zn O | 第68-69页 |
| 4.4.2 AR and LC of AKBA on Zn O particles | 第69-70页 |
| 4.4.3 UV-controlled release behavior | 第70-71页 |
| 4.4.4 AKBA modulates UVA-induced inflammatory mediators | 第71-75页 |
| 4.4.5 Cytotoxicity of AKBA@Zn O | 第75-76页 |
| 4.4.6 Cytotoxicity of AKBA@Zn O on human FEK-4 fibroblast cells | 第76-78页 |
| 4.4.7 UVA generated Oxidative stressand effect of AKBA on FEK-4 Fibroblast cells | 第78页 |
| 4.4.8 The Lactate Dehydrogenase (LDH) Leakage assay | 第78-80页 |
| 4.4.9 The effect of AKBA on UVA-mediated MMP-1, TIMP-1, Col-III expression in HaCaTand FEK-4 fibroblast cells | 第80页 |
| 4.4.10 EFFECT OF AKBA ON UVA-INDUCED MMP-1 EXPRESSION IN HACAT CELLS | 第80-81页 |
| 4.5 DISCUSSION | 第81-82页 |
| 4.6 CONCLUSION | 第82-83页 |
| 5. THE EXPRESSION OF ANTI-OXIDANT ENZYMES OR STRESSRESPONSE IN HACAT CELLS | 第83-93页 |
| 5.1 INTRODUCTION | 第83-84页 |
| 5.2 MATERIALS AND METHODS | 第84-85页 |
| 5.2.1 Materials, reagents and antibodies | 第84页 |
| 5.2.2 Preparation of some Reagents | 第84页 |
| 5.2.3 Cell cultures and UVA dosimetry | 第84页 |
| 5.2.4 Western Blotting | 第84页 |
| 5.2.5 Developing | 第84-85页 |
| 5.3 RESULTS | 第85-89页 |
| 5.3.1 Activation of Nrf2/Bach pathway by UVA irradiation in HaCaT cells | 第85页 |
| 5.3.2 Effect of ER on Nrf2/Bach pathway in UVA exposed HaCaT cells | 第85-86页 |
| 5.3.3 Effect of BBR on Nrf2/Bach expression in UVA exposed HaCaT cells | 第86-87页 |
| 5.3.4 Effect of BBR (5 μM) on expression of oxidative stress response proteins at various time points | 第87-88页 |
| 5.3.5 Effect of AKBA on phosphorylation of e IF2α in FEK-4 fibroblast cells | 第88-89页 |
| 5.3.6 Effect of AKBA on NRF2/Bach1 pathway in UVA irradiated FEK-4 fibroblast cells | 第89页 |
| 5.4 DISCUSSION | 第89-91页 |
| 5.5 CONCLUSION | 第91-93页 |
| 6. GENERAL DISCUSSION | 第93-97页 |
| ACKNOWLEDGEMENT | 第97-99页 |
| REFERENCES | 第99-113页 |
| APPENDIX RESEARCH PAPERS PUBISHED DURING PH.D. STUDY | 第113-114页 |
