| 摘要 | 第1-6页 |
| Abstract | 第6-7页 |
| Chapter Ⅰ Background and Introduction | 第7-24页 |
| Background on Thrombus | 第7-8页 |
| Coagulation Pathway | 第8-11页 |
| Thrombolysis Pathway and Therapy of Thrombosis | 第11-16页 |
| Drug Design and Research Aims | 第16-20页 |
| Reference | 第20-24页 |
| Chapter Ⅱ Expression of fusion protein of staphylokinase with hirudin in Pichia pastoris and mutation of amino acids of staphylokinase between Asn28 and Thr30 dramatically decreasing fibrinolytic activity | 第24-39页 |
| Introduction | 第24-25页 |
| Materials and Methods | 第25-29页 |
| Results and Discussion | 第29-37页 |
| Mutagenesis of sale, construction of pPIC9K-SFH and transformation of GS115 | 第29-33页 |
| Protein SFH expression in P. pastoris | 第33-34页 |
| Biological activity of purified rSFH | 第34-36页 |
| Assay relationship between structure and function of SAK | 第36-37页 |
| Reference | 第37-39页 |
| Chapter Ⅲ Expression of fusion protein by E. coli and its characters of targeting property and bifunctional activities | 第39-59页 |
| Introduction | 第39页 |
| Materials and Methods | 第39-44页 |
| Results | 第44-52页 |
| Protein expression and purification | 第44-45页 |
| Activation of plasminogen and fibrinolytic activity | 第45-48页 |
| Thrombin-targeting activity and anticoagulant activity | 第48-49页 |
| FXa activating fusion protein to staphylokinase and hirudin | 第49-51页 |
| SFH anticoagulant activity in vivo | 第51-52页 |
| Discussion | 第52-55页 |
| Conclusion | 第55-57页 |
| References | 第57-59页 |
| Appendix Acknowledge | 第59-69页 |
