| 摘要 | 第1-7页 |
| ABSTRACT | 第7-14页 |
| Chapter 1 Introduction | 第14-23页 |
| ·Research interests | 第14-17页 |
| ·Background information | 第17-21页 |
| ·Historical context | 第17页 |
| ·Stored grain | 第17-18页 |
| ·Stored grain insects | 第18-19页 |
| ·Volatile chemicals from stored grain and stored grain insects | 第19页 |
| ·Problems associated with stored grain and stored grain pests | 第19-20页 |
| ·Diagnosis and inspection | 第20-21页 |
| ·Study aims | 第21-23页 |
| Chapter 2 Literature Review | 第23-40页 |
| ·Problems associated with stored grain | 第23-25页 |
| ·Techniques of detecting and analysis | 第25-30页 |
| ·Development history | 第25-28页 |
| ·Merits and drawbacks | 第28-30页 |
| ·Target species | 第30-32页 |
| ·Tribolium castaneum (Herbst) | 第30-31页 |
| ·Rhyzopertha dominica (F.) | 第31页 |
| ·Sitophilus granarius (Linnaeus) | 第31-32页 |
| ·Volatiles of stored grain and pests | 第32-39页 |
| ·Identification of volatiles | 第32-37页 |
| ·Electrophysiological screening and behavioural confirmation | 第37-38页 |
| ·General use of volatiles from stored grain and stored grain pests | 第38-39页 |
| ·Conclusions and perspectives | 第39-40页 |
| Chapter 3 Materials and Methods | 第40-46页 |
| ·Materials | 第40页 |
| ·Insect culture | 第40页 |
| ·Wheat variety and moisture content (wet basis) | 第40页 |
| ·Reagents and apparatus | 第40页 |
| ·Primary experiment | 第40-42页 |
| ·Fibre Selection and HS-SPME Extraction Time | 第40-41页 |
| ·Selection of sampling time | 第41页 |
| ·Preparation of standards | 第41-42页 |
| ·Experiment design | 第42-44页 |
| ·Influence of storage conditions on volatile compounds from wheat alone | 第42-43页 |
| ·Single-species experiments | 第43页 |
| ·Electrophysiological responses to odours | 第43-44页 |
| ·Behavioural biossay | 第44页 |
| ·Preparation of stimuli | 第44页 |
| ·Statistical analysis | 第44-45页 |
| ·Results | 第45-46页 |
| Chapter 4 Volatile Chemicals from Wheat and Flour | 第46-69页 |
| ·Introduction | 第46-47页 |
| ·Materials and methods | 第47页 |
| ·Results | 第47-62页 |
| ·Identification of main components in wheat grain headspace | 第47-48页 |
| ·Effects of storage time on volatile compounds from wheat grain with 12.3% m.c. under 15°C | 第48-50页 |
| ·Effects of storage time on volatile compounds from wheat grain with 12.3% m.c. under 25°C | 第50-52页 |
| ·Effects of storage time on volatile compounds from wheat grain with 12.3% m.c. under 35°C | 第52-54页 |
| ·Effects of storage temperature on volatile compounds from wheat grain with 12.3% m.c | 第54-56页 |
| ·Effects of storage time on volatile compounds from wheat grain with 14.3% moisture content at 15 oC | 第56-57页 |
| ·Effects of storage time on volatile compounds from wheat grain with 14.3% moisture content at 25oC | 第57-60页 |
| ·Effects of storage time on volatile compounds from wheat grain with 14.3% moisture content at 35oC | 第60页 |
| ·Effects of storage temperature on volatile compounds from wheat grain with 14.3% m.c | 第60-62页 |
| ·Comparison of effects of moisture content on volatile profile of wheat | 第62-68页 |
| ·Conclusions & Discussions | 第68-69页 |
| Chapter 5 Volatile Chemicals from Insects Only | 第69-76页 |
| ·Introduction | 第69-70页 |
| ·Materials and methods | 第70-71页 |
| ·Insect culture | 第70页 |
| ·Experiment design | 第70-71页 |
| ·GC and GC-MS analysis | 第71页 |
| ·Results | 第71-75页 |
| ·Volatile profile of T. castaneum | 第71-73页 |
| ·Volatile profile of R. dominica | 第73-75页 |
| ·Volatile profile of S. granarius | 第75页 |
| ·Conclusions and discussions | 第75-76页 |
| Chapter 6 Volatile Chemicals from Wheat plus Insects | 第76-81页 |
| ·Introduction | 第76-77页 |
| ·Materials and methods | 第77-78页 |
| ·Insect culture | 第77页 |
| ·Selection of sampling time | 第77页 |
| ·Wheat plus single-species experiments | 第77-78页 |
| ·GC and GC-MS analysis | 第78页 |
| ·Results | 第78-80页 |
| ·Identification of volatile compounds from wheat only | 第78-80页 |
| ·Identification of volatile compounds from wheat plus T. castaneum, R. dominica,and S. granarius | 第80页 |
| ·Discussion | 第80-81页 |
| Chapter 7 Bioassay and Behaviour Study | 第81-95页 |
| ·Introduction | 第81-82页 |
| ·Materials and methods | 第82-85页 |
| ·Results and conclusions | 第85-93页 |
| ·Behavioural bioassay of Tribolium castaneum adults to odors | 第85-89页 |
| ·Behavioural bioassay of T. castaneum larvae to odors | 第89-90页 |
| ·EAG responses of T. castaneum to volatile stimuli | 第90-92页 |
| ·Behavioural bioassay of Sitophilus granarius | 第92页 |
| ·EAG responses of S. granarius to volatile stimuli | 第92-93页 |
| ·Conclusions and Discussions | 第93-95页 |
| Chapter 8 Conclusions and Discussion | 第95-100页 |
| References | 第100-110页 |
| Acknowledgement | 第110-113页 |
| Curriculum Vitae | 第113-114页 |
