| ABSTRACT | 第1-7页 |
| 摘要 | 第7-11页 |
| Chapter 1 Literature Review | 第11-23页 |
| ·Insect olfactory system | 第11-21页 |
| ·Insect sensillum | 第11-12页 |
| ·Perireceptor events in insect olfaction | 第12-15页 |
| ·Structure of proteins involved in insect olfaction | 第15-16页 |
| ·Principles of insect olfaction | 第16-19页 |
| ·Tissue expression of olfactory proteins | 第19-20页 |
| ·Interaction of semiochemicals with odorant binding proteins | 第20-21页 |
| ·Navel orangewom and its damage | 第21-23页 |
| Chapter 2 Electrophysiological Response to Pheromones and Plant Volatile Chemicals | 第23-33页 |
| ·Introduction | 第23页 |
| ·Materials and methods | 第23-26页 |
| ·Insects | 第23-24页 |
| ·Electroantennogram (EAG) recordings | 第24-25页 |
| ·Chemical stimuli | 第25-26页 |
| ·Results | 第26-31页 |
| ·Female A transitella EAG response to plant volatiles at different concentration | 第26-29页 |
| ·Male A. transitella EAG response to plant volatile chemicals | 第29-31页 |
| ·Conclusions and discussions | 第31-33页 |
| Chapter 3 Protein Expression and Purification | 第33-55页 |
| ·Introduction | 第33页 |
| ·Materials and methods | 第33-42页 |
| ·Protein expression and purification | 第33-41页 |
| ·Circular dichroism | 第41-42页 |
| ·Results | 第42-53页 |
| ·Result of protein purification | 第42-43页 |
| ·Result of large scale inoculation and harvest cells | 第43-44页 |
| ·Result of protein purification | 第44-53页 |
| ·Discussion | 第53-55页 |
| Chapter 4 Affinity of Plant Volatile Chemicals to General Odorant Binding Proteins | 第55-80页 |
| ·Introduction | 第55页 |
| ·Materials and methods | 第55-58页 |
| ·Cold binding assay | 第55-57页 |
| ·Fluorescence binding assay | 第57-58页 |
| ·Results | 第58-79页 |
| ·Result of cold binding assay | 第58-63页 |
| ·Result of fluorescence binding assay | 第63-79页 |
| ·Discussion and conclusion | 第79-80页 |
| Chapter 5 Effects of Plant Volatile Chemicals on Navel Orangeworm OvipositionBehavior | 第80-86页 |
| ·Introduction | 第80页 |
| ·Materials and methods | 第80-82页 |
| ·Insects | 第80-81页 |
| ·Trap preparation | 第81页 |
| ·Behavior observation | 第81-82页 |
| ·Field examination of oviposition attraction of tested chemicals | 第82页 |
| ·Results | 第82-85页 |
| ·Effect of plant volatile to navel orangeworm | 第82页 |
| ·Comparison of different chemicals attraction | 第82-83页 |
| ·Attraction comparison of plant volatile in the field | 第83-85页 |
| ·Discussion | 第85-86页 |
| Chapter 6 Conclusions and Discussions | 第86-89页 |
| References | 第89-98页 |
| Acknowledgements | 第98-99页 |
| Curriculum Vitae | 第99页 |
