Abstract | 第1-6页 |
摘要 | 第6-10页 |
General introduction | 第10-22页 |
·Background on Panax ginseng | 第10-13页 |
·Etymology, botanical position, and history of Panax ginseng | 第10-12页 |
·Classifications of Panax | 第12页 |
·Reproductive system, growth, and cultivation of ginseng | 第12-13页 |
·Importance of Panax ginseng | 第13-15页 |
·Medicinal importance of Panax ginseng | 第13-15页 |
·Bioactive compounds of Panax ginseng C.A. Meyer | 第15页 |
·Economic importance of ginseng | 第15页 |
·Genomic analyses of ginseng | 第15-17页 |
·Genetic diversity among and between plant populations | 第17-18页 |
·DNA methylation polymorphisms among and between plant populations | 第18-19页 |
·Aims and scope of the present work | 第19-22页 |
Materials and Methods | 第22-28页 |
·Plant material | 第22页 |
·Methods | 第22-28页 |
·Total genomic DNA extraction and purification | 第22-23页 |
·AFLP analysis | 第23-25页 |
·MSAP analysis | 第25-26页 |
·Band scoring and statistical data analysis | 第26-27页 |
·Isolation, cloning, and sequencing of AFLP and MSAP variable bands | 第27页 |
·Bisulfite conversion and sequencing | 第27-28页 |
Results analysis | 第28-47页 |
·AFLP polymorphisms | 第28-31页 |
·AFLP-based genetic structure of ginseng groups | 第30-31页 |
·MSAP analysis | 第31-41页 |
·Methylation levels in the studied groups of ginseng plants | 第31-35页 |
·Methylation-insensitive polymorphisms | 第35-36页 |
·MIP-based genetic structure of ginseng groups | 第36-38页 |
·Methylation-sensitive polymorphisms (MSP) | 第38-39页 |
·Epigenetic structure of ginseng groups | 第39-41页 |
·Summary of the indices of genetic and epigenetic diversity between wild and cultivated ginseng | 第41页 |
·Mantel test for correlations between genetic and epigenetic polymorphisms | 第41-42页 |
·Sequences of the AFLP and MSAP variants indicative of genetic and epigenetic polymorphisms | 第42-43页 |
·Validation of cytosine methylation alteration by bisulfite sequencing | 第43-47页 |
Discussion | 第47-51页 |
·Absence of clear genetic difference between wild and cultivated ginseng plants | 第47-48页 |
·Epigenetic distinctness between wild and cultivated ginseng plants | 第48-51页 |
Conclusions | 第51-52页 |
References | 第52-65页 |
Acknowledgements | 第65-66页 |
List of Publications | 第66页 |