| 摘要 | 第9-12页 |
| ABSTRACT | 第12-15页 |
| LIST OF ABBREVIATIONS | 第16-19页 |
| CHAPTER 1 INTRODUCTION | 第19-23页 |
| 1. The need for studying the Sertoli cell in the testis of Pelodiscus sinensis | 第19-20页 |
| 2. Research goals | 第20-23页 |
| CHAPTER 2 LITERATURE REVIEW | 第23-45页 |
| Part Ⅰ Introduction to Experimental Animal | 第23-24页 |
| 1. Brief history of experimental animal | 第23页 |
| 2. Significance of Chinese soft shelled turtle | 第23页 |
| 3. Life cycle | 第23-24页 |
| Part Ⅱ The Male Reproductive Tract and Spermatogenesis | 第24-28页 |
| 1. Testes | 第24-28页 |
| 1.1 The germinal epithelium and spermatogenesis in fishes and amphibians testis | 第24-25页 |
| 1.2 The germinal epithelium and spermatogenesis in mammals and birds testis | 第25-26页 |
| 1.3 The germinal epithelium and spermatogenesis in reptilian testis | 第26-27页 |
| 1.4 Types of seasonal cycles in reptiles | 第27-28页 |
| Part Ⅲ Sertoli cell | 第28-37页 |
| 1. Ultrastructure of Sertoli cell | 第28页 |
| 2. Sertoli cell is necessary for the development of testis and regulation of spermatogenesis | 第28-29页 |
| 3. Sertoli cell regulates endocrine during spermatogenesis | 第29页 |
| 4. Sertoli cell produce lipid droplets during spermatogenesis | 第29-30页 |
| 5. Junctional complexes amongst Sertoli cell and germ cells | 第30-33页 |
| 5.1 Inter-Sertoli cells tight junctions | 第31-32页 |
| 5.2 Adherens junctions | 第32页 |
| 5.3 Gap junctions | 第32-33页 |
| 6. Phagocytosis role of Sertoli cells | 第33-37页 |
| 6.1 Autophagy in Sertoli cell | 第34-35页 |
| 6.2 Entosis | 第35-37页 |
| REFERENCES | 第37-45页 |
| CHAPTER 3 (EXPERIMENT 1) CYTOLOGICAL STUDY ON SERTOLI CELLS AND THEIRINTERACTIONS WITH GERM CELLS DURING ANNUAL REPRODUCTIVE CYCLE INTURTLE | 第45-69页 |
| 1. Introduction | 第45-47页 |
| 2. Materials and Methods | 第47-48页 |
| 2.1 Experimental Animals and Ethical Approval | 第47页 |
| 2.2 Light Microscopy | 第47页 |
| 2.3 Transmission Electron Microscopy (TEM) | 第47-48页 |
| 2.4 Immunohistochemistry for vimentin | 第48页 |
| 3. Results | 第48-60页 |
| 3.1 General histology of testis of Pelodiscus sinensis | 第48-49页 |
| 3.2 Localization of vimentin in the testis of Pelodiscus sinensis | 第49页 |
| 3.3 Ultrastructure of Sertoli cell in testis of Pelodiscus sinensis | 第49-60页 |
| 4. Discussion | 第60-63页 |
| 5. Conclusion | 第63-64页 |
| REFERENCES | 第64-69页 |
| CHAPTER 4 (EXPERIMENT 2) CHARACTERIZATION OF INTER-SERTOLI CELL TIGHTJUNCTIONS AND GAP JUNCTIONS IN THE TESTIS OF CHINESE SOFT-SHELLEDTURTLE | 第69-89页 |
| 1. Introduction | 第69-71页 |
| 2. Materials and Methods | 第71-72页 |
| 2.1 Experimental Animals and Ethical Approval | 第71页 |
| 2.2 Immunohistochemistry for CLDN11 and Cx43 | 第71-72页 |
| 2.3 Fluorescence Microscopy | 第72页 |
| 2.4 Transmission Electron Microscopy (TEM) | 第72页 |
| 3. Results | 第72-82页 |
| 3.1 Localization of inter-Sertoli cells tight junction protein CLDN11 in Pelodiscus sinensis testis | 第72-73页 |
| 3.2 Localization of gap junction protein Cx43 in Pelodiscus sinensis testis | 第73-74页 |
| 3.3 Ultrastructure of various junctions in Pelodiscus sinensis testis | 第74-82页 |
| 4. Discussion | 第82-84页 |
| 5. Conclusion | 第84-85页 |
| REFERENCES | 第85-89页 |
| CHAPTER 5 (EXPERIMENT 3) NOVEL CELLULAR EVIDENCE OF LIPOPHAGY WITHINTHE SERTOLI CELLS DURING SPERMATOGENESIS IN THE TURTLE | 第89-107页 |
| 1. Introduction | 第89-91页 |
| 2. Materials and methods | 第91-92页 |
| 2.1 Experimental Animals and Ethical Approval | 第91页 |
| 2.2 Light Microscopy | 第91页 |
| 2.3 Immunohistochemistry for LC3 | 第91-92页 |
| 2.4 Oil Red O (ORO) Staining | 第92页 |
| 2.5 Transmission Electron Microscopy (TEM) | 第92页 |
| 3. Results | 第92-100页 |
| 3.1 General cytology of germ cells and Sertoli cells in the testis of Pelodiscus sinensis | 第92-93页 |
| 3.2 Localization of LC3 in the testis of Pelodiscus sinensis | 第93页 |
| 3.3 Oil red O (ORO) Staining for localization of lipid droplets in the Sertoli cell | 第93页 |
| 3.4 Ultrastrucutre of germ cells along with Sertoli cells during spermatogenesis | 第93-100页 |
| 4. Discussion | 第100-102页 |
| 5. Conclusion | 第102-104页 |
| REFERENCES | 第104-107页 |
| CHAPTER 6 (EXPERIMENT 4) ENTOSIS ACTS AS A NOVEL WAY WITHIN SERTOLICELLS TO ELIMINATE SPERMATOZOA IN SEMINIFEROUS TUBULE | 第107-125页 |
| 1. Introduction | 第107-109页 |
| 2. Materials and Methods | 第109-110页 |
| 2.1 Experimental Animals and Ethical Approval | 第109页 |
| 2.2 Transmission Electron Microscopy | 第109页 |
| 2.3 Immunohistochemistry of LAMP-1 | 第109-110页 |
| 2.4 Western Blot Analysis of LAMP-1 | 第110页 |
| 2.5 Statistical Analysis | 第110页 |
| 3. Results | 第110-120页 |
| 3.1 Lysosomal membrane protein (LAMP1) was expressed in the testis, being specifically locatedinside Sertoli cells | 第111页 |
| 3.2 Various entotic vacuoles occurred within Sertoli cells during hibernation | 第111-112页 |
| 3.3 Numerous different autophagosomes were observed by TEM within the entotic vacuoles ofSertoli cells | 第112页 |
| 3.4 Clear adherens junctions were observed between developing germ cells and Sertoli cells | 第112-120页 |
| 4. Discussion | 第120-123页 |
| REFERENCES | 第123-125页 |
| SUMMARY | 第125-127页 |
| CONCLUSION | 第127-129页 |
| INNOVATIONS | 第129-131页 |
| FUTURE RESEARCH DIRECTIONS | 第131-133页 |
| PUBLICATIONS | 第133-135页 |
| ACKNOWLEDGEMENTS | 第135页 |
