| 摘要 | 第6-7页 |
| Abstract | 第7-8页 |
| CHAPTER 1. INTRODUCTION | 第16-28页 |
| 1.0 The honeybee | 第16页 |
| 1.1 History of Beekeeping Industry | 第16-17页 |
| 1.2 Beekeeping Industry in China | 第17-18页 |
| 1.3 Economic and ecologic importance of honeybees | 第18-21页 |
| 1.4 Honeybee colony organization and caste dependent roles | 第21-24页 |
| 1.4.1 Queen | 第22-23页 |
| 1.4.2 Drones | 第23页 |
| 1.4.3 Workers | 第23-24页 |
| 1.5 Anatomy of the Honeybee | 第24-28页 |
| 1.5.1 The Antennae | 第25-28页 |
| CHAPTER 2. QUALITATIVE AND QUANTITATIVE ANTENNAL PROTEOME COMPARISON OF ITALIAN HONEYBEES (APIS MELLIFERA) ACROSS DIFFERENT AGES | 第28-47页 |
| 2.1 Summary | 第28页 |
| 2.2 Introduction | 第28-30页 |
| 2.3 Objective | 第30页 |
| 2.4 Materials and Methods | 第30-33页 |
| 2.4.1 Chemical reagents | 第30页 |
| 2.4.2 Sampling of antennae | 第30-31页 |
| 2.4.3 Protein extraction and digestion | 第31页 |
| 2.4.5 LC-MS/MS analysis | 第31-32页 |
| 2.4.6 Protein identification and quantification of abundance | 第32-33页 |
| 2.4.7 Bioinformatics analysis | 第33页 |
| 2.5 Results | 第33-42页 |
| 2.5.1 Qualitative antennal proteome comparison of adult ITB workers across three time points | 第35-39页 |
| 2.5.2 Quantitative antennal proteome comparison of adult worker ITBs across three time points | 第39-42页 |
| 2.6 Discussion | 第42-45页 |
| 2.6.1 Olfactory function of adult worker bees requires shared antennal proteome | 第43页 |
| 2.6.2 Antennal proteome dynamics coordinate age-related behavioural physiology of adult worker bees | 第43-45页 |
| 2.7 Conclusion | 第45-47页 |
| CHAPTER 3. QUALITATIVE AND QUANTITATIVE ANTENNAL PROTEOME COMPARISON OF ROYAL JELLY BEES (APIS MELLIFERA LIGUSTICA) ACROSS DIFFERENT AGES | 第47-63页 |
| 3.1 Summary | 第47页 |
| 3.2 Introduction | 第47-48页 |
| 3.3 Objective | 第48-49页 |
| 3.4 Materials and Methods | 第49页 |
| 3.4.1 Chemical reagents | 第49页 |
| 3.4.2 Antennae sampling | 第49页 |
| 3.5 Results | 第49-58页 |
| 3.5.1 Qualitative antennal proteome comparison of RJB workers across three time points | 第50-55页 |
| 3.5.2 Quantitative antennal proteome comparison of RJB workers over three time points | 第55-58页 |
| 3.6 Discussion | 第58-61页 |
| 3.6.1 Olfactory function of adult workers requires shared antennal proteome | 第59页 |
| 3.6.2 Adult worker bees evolve age-dependent antennal proteome to prime age-related biology | 第59-61页 |
| 3.7 Conclusion | 第61-63页 |
| CHAPTER 4. ANTENNAL PROTEOME COMPARISON BETWEEN TWO STRAINS OF HONEYBEES REVEALS DEEP-INSIGHT INTO MOLECULAR UNDERPINNINGS OF OLFACTORY BEHAVIOUR OF WORKER BEES | 第63-80页 |
| 4.1 Summary | 第63页 |
| 4.2 Introduction | 第63-66页 |
| 4.3 Objective | 第66页 |
| 4.4 Materials and Methods | 第66-67页 |
| 4.4.1 Chemical Reagents | 第66页 |
| 4.4.2 Antennae sampling from ITBs and RJBs | 第66-67页 |
| 4.5 Results | 第67-77页 |
| 4.5.1 Antennal proteome comparison between ITBs and RJBs at NEB stage | 第67-70页 |
| 4.5.2 Antennal proteome comparison between ITBs and RJBs at NB stage | 第70-73页 |
| 4.5.3 Antennal proteome comparison between ITBs and RJBs at FB stage | 第73-77页 |
| 4.6 Discussion | 第77-80页 |
| 4.6.1 Basic functionality of olfactory system requires a shared proteome | 第77-78页 |
| 4.6.2 ITBs and RJBs adapt divergent antennal proteome architectures during age-related polyethism | 第78-80页 |
| 4.7 CONCLUSION | 第80页 |
| GENERAL CONCLUSION | 第80-82页 |
| REFERENCES | 第82-95页 |
| List of Tables | 第95-116页 |
| ACKNOWLEDGEMENTS | 第116-117页 |
| AUTHOR’S BIOGRAPHY | 第117-119页 |
