| 摘要 | 第9-11页 |
| ABSTRACT | 第11-13页 |
| ABBREVIATIONS | 第14-15页 |
| CHAPTER 1 Introduction | 第15-25页 |
| 1.1 Normal Growth Plate | 第15-16页 |
| 1.2 Avian Growth Plate | 第16-17页 |
| 1.3 Growth Plate Angiogenesis | 第17-18页 |
| 1.4 Role of Extra Cellular Matrix | 第18-20页 |
| 1.5 Tibial Dyschondroplasia | 第20-21页 |
| 1.6 Vascularization and ECM-degradation in TD | 第21-23页 |
| 1.7 Tools for TD Research | 第23页 |
| 1.7.1 Field TD | 第23页 |
| 1.7.2 Induced TD | 第23页 |
| 1.7.3 Cell cultures | 第23页 |
| 1.8 Conclusion and Hypothesis | 第23-25页 |
| CHAPTER 2 Differential Expression of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN/CD147) in Avian Tibial Dyschondroplasia | 第25-36页 |
| 2.1 Objectives | 第25页 |
| 2.2 Materials and Methods | 第25-27页 |
| 2.2.1 Animal welfare & ethics | 第25页 |
| 2.2.2 Experimental design and induction of tibial dyschondroplasia | 第25-26页 |
| 2.2.3 Immunohistochemistry for CD147 | 第26页 |
| 2.2.4 RNA extraction and reverse transcription | 第26页 |
| 2.2.5 Quantitative real-time polymerase chain reaction | 第26页 |
| 2.2.6 Western blot analysis | 第26-27页 |
| 2.2.7 Statistical analysis | 第27页 |
| 2.3 Results | 第27-33页 |
| 2.3.1 Morphological analysis of TD affected and recovered chickens and growth plates | 第27-28页 |
| 2.3.2 Gene expression and protein levels of CD147/EMMPRIN | 第28-32页 |
| 2.3.3 Expression of MMP-9, heat shock protein 90 and vascular endothelial growth factor genes | 第32-33页 |
| 2.4 Discussion | 第33-36页 |
| CHAPTER 3 Effect of Hsp-90 Inhibition on Extracellular Matrix Degradation in Thiram-Induced Avian Tibial Dyschondroplasia | 第36-45页 |
| 3.1 Objectives | 第36页 |
| 3.2 Materials and Methods | 第36-38页 |
| 3.2.1 Animal welfare & ethics | 第36页 |
| 3.2.2 Experimental design and induction of tibial dyschondroplasia | 第36-37页 |
| 3.2.3 RNA extraction and reverse transcription | 第37页 |
| 3.2.4 Quantitative real-time polymerase chain reaction | 第37页 |
| 3.2.5 Extraction of proteins | 第37页 |
| 3.2.6 Western blot (WB) analysis | 第37-38页 |
| 3.2.7 Gelatin zymography (GZ) | 第38页 |
| 3.2.8 Statistical analysis | 第38页 |
| 3.3 Results | 第38-42页 |
| 3.3.1 Hsp-90 inhibition and TD prevention | 第38-39页 |
| 3.3.2 Hsp-90 inhibition and vascularization in TD prevention | 第39页 |
| 3.3.3 Hsp-90 inhibition and ECM degradation in TD prevention | 第39-41页 |
| 3.3.4 Hsp-90 inhibition and established TD | 第41-42页 |
| 3.4 Discussion | 第42-45页 |
| CHAPTER 4 Hsp-90 Inhibitor Geldanamycin Attenuates Liver Oxidative Stress and Toxicity in Thiram-Induced Tibial Dyschondroplasia | 第45-50页 |
| 4.1 Objectives | 第45页 |
| 4.2 Materials and Methods | 第45-46页 |
| 4.2.1 Animal welfare & ethics | 第45页 |
| 4.2.2 Chickens and experimental design | 第45页 |
| 4.2.3 Samples preparation | 第45-46页 |
| 4.2.4 Tissue preparation and liver enzymatic activity determination | 第46页 |
| 4.2.5 Plasma and serum biochemical assay | 第46页 |
| 4.2.6 Statistical analysis | 第46页 |
| 4.3 Results | 第46-48页 |
| 4.4 Discussion | 第48-50页 |
| CHAPTER 5 Expression of Genes encoding Matrilin-3 and Cyclin-I during the Impairment and Recovery of Chicken Growth Plate in Tibial Dyschondroplasia | 第50-59页 |
| 5.1 Objectives | 第50页 |
| 5.2 Materials and Methods | 第50-53页 |
| 5.2.1 Animal welfare & ethics | 第50页 |
| 5.2.2 Experimental design and induction of TD | 第50-51页 |
| 5.2.3 Designing of probes | 第51页 |
| 5.2.4 Histological staining, immunohistochemistry (IHC), and in situ hybridization of growth plate sections | 第51-52页 |
| 5.2.5 RNA extraction and reverse transcription | 第52页 |
| 5.2.6 Quantitative real-time PCR (qRT-PCR) | 第52页 |
| 5.2.7 Statistical analysis | 第52-53页 |
| 5.3 Results | 第53-57页 |
| 5.3.1 Morphological analysis of TD affected and recovered chickens and growth plates | 第53页 |
| 5.3.2 ISH and IHC | 第53-57页 |
| 5.3.3 Expression of Matrilin-3 and Cyclin-I genes | 第57页 |
| 5.4 Discussion | 第57-59页 |
| CHAPTER 6 Conclusion and Future Prospects | 第59-61页 |
| 6.1 Study I | 第59页 |
| 6.2 Study II | 第59页 |
| 6.3 Study III | 第59-60页 |
| 6.4 Study IV | 第60页 |
| 6.5 Future Prospects | 第60-61页 |
| CHAPTER 7 References | 第61-72页 |
| CHAPTER 8 List of Figures | 第72-73页 |
| CHAPTER 9 List of Tables | 第73-74页 |
| CHAPTER 10 Acknowledgements | 第74-76页 |
| Appendix I Scientific Contributions | 第76-78页 |
