| Abstract in Chinese | 第1-13页 |
| Abstract in English | 第13-17页 |
| Chapter 1 Introduction | 第17-44页 |
| 1．1 The structure, synthesis, properties and application of Metallophthalocyanine Compounds | 第17-28页 |
| 1．1．1 Introduction | 第17页 |
| 1．1．2 The molecular structure of phthalocyanine compounds | 第17-18页 |
| 1．1．3 Synthesis of metallophthalocyanine compounds | 第18-19页 |
| 1．1．4 The general properties of phthalocyanine compounds | 第19-20页 |
| 1．1．5 The spectral characteristics of phthalocyanine compounds | 第20-21页 |
| 1．1．6 Applications of phthalocyanine compounds | 第21-28页 |
| 1．1．6．1 Application of phthalocyanine compounds as a catalyst | 第22-25页 |
| 1．1．6．1．1 Application of metallophthalocyanine compounds as a eletrocatalysts in electrochemical analysis | 第22页 |
| 1．1．6．1．2 Application of metallophthalocyanines as a mimetic peroxidase in fluorescence analysis | 第22-25页 |
| 1．1．6．2 Medical applications | 第25-26页 |
| 1．1．6．3 Analytical application | 第26-28页 |
| 1．2 Development of fluorogenic substrates for HRP and HRP Mimics | 第28-31页 |
| 1．2．1 Fluorophores of 2, 2 '-dihydroxydiphenyl derivatives | 第28-29页 |
| 1．2．2 Fluorescence quenching substrates | 第29页 |
| 1．2．3 Other substrates | 第29-31页 |
| 1．3 Study of enzymes-catalyzed reactions in reversed mieelles | 第31-35页 |
| 1．3．1 Characteristics of reversed micelles | 第31-32页 |
| 1．3．2 The catalytic activity of enzyme in reversed micelles | 第32-34页 |
| 1．3．2．1 Influence of W value on the catalytic activity of enzymes | 第32-33页 |
| 1．3．2．2 Influence of surfactant concentration oncatalyti cactivity of enzymes | 第33-34页 |
| 1．3．3 Progress of the study on peroxidase in reversed micelles | 第34-35页 |
| 1．4 The objective of this dissertation | 第35-36页 |
| Referenees | 第36-44页 |
| Chapter 2 Study of tetra-substituted amino aluminum phthalocyanine as a new red-region substrate for HRP and its analytical application | 第44-67页 |
| 2．1 Introduction | 第44-45页 |
| 2．2 Materials and apparatus | 第45-46页 |
| 2．2．1 Materials | 第45-46页 |
| 2．2．2 Apparatus | 第46页 |
| 2．3 Investigation on the potential use of TAAIPc as a fluorescent substrate for HRP | 第46-50页 |
| 2．3．1 Experimental | 第46-47页 |
| 2．3．1．1 Procedure for the determination of the catalytic activity of HRP with TAAIPc as a substrate | 第46-47页 |
| 2．3．2 Results and discussion | 第47-50页 |
| 2．3．2．1 Spectral characteristics | 第47-48页 |
| 2．3．2．2 Measurement of constant of enzymatic reaction | 第48-50页 |
| 2．3．3 Conclusion | 第50页 |
| 2．4 Application of TAAIPc as a fluorogenic indicator to the determination of H_2O_2 | 第50-59页 |
| 2．4．1 Application of HRP-catalyzed reaction of H_2O_2 and TAAIPc to the determination of H_2O_2 and glucose in human serum | 第50-55页 |
| 2．4．1．1 Experimental | 第50页 |
| 2．4．1．1．1 Assay procedure for H_2O_2 | 第50页 |
| 2．4．1．1．2 Procedure for glucose determination | 第50页 |
| 2．4．1．2 Results and disicussion | 第50-55页 |
| 2．4．1．2．1 Spectral characteristics | 第50-51页 |
| 2．4．1．2．2 Optimization of experimental parameters | 第51-53页 |
| 2．4．1．2．2．1 Effect of pH and buffer | 第51-52页 |
| 2．4．1．2．2．2 Reaction time and temperature | 第52页 |
| 2．4．1．2．2．3 Effects of reagent concentrations | 第52-53页 |
| 2．4．1．2．3 Analytical performance | 第53页 |
| 2．4．1．2．4 Effect of foreign substances | 第53-54页 |
| 2．4．1．2．5 Determination of glucose in serum samples | 第54-55页 |
| 2．4．2 Study of TAAIPc as a fluorogenic substrate in a HRP mimiccatalyzed oxidation reaction for the determination of H_2O_2 | 第55-59页 |
| 2．4．2．1 Experimental | 第55页 |
| 2．4．2．1．1 Analysis of H_2O_2 using heroin as the catalyst | 第55页 |
| 2．4．2．1．2 Analysis of H_2O_2 using FeTSPc as the catalyst | 第55页 |
| 2．4．2．2 Results and discussion | 第55-59页 |
| 2．4．2．2．1 Spectral characteristics | 第55-56页 |
| 2．4．2．2．2 Optimization of experimental parameters | 第56-57页 |
| 2．4．2．2．2．1 Effect of pH and buffer | 第56页 |
| 2．4．2．2．2．2 Reaction time and temperature | 第56-57页 |
| 2．4．2．2．2．3 Optimum amounts of catalyst and TAAIPc | 第57页 |
| 2．4．2．2．3 Features of the analytical method | 第57-58页 |
| 2．4．2．2．4 Effects of foreign substances | 第58页 |
| 2．4．2．2．5 Determination of H_2O_2 in rainwater samples | 第58-59页 |
| 2．4．3 Conclusions | 第59页 |
| 2．5 Application of TAAIPe as a fluorogenie substrate in peroxidase-mediated enzyme-linked immunosorbent assay | 第59-65页 |
| 2．5．1 A competitive immunoassay method for a-l-fetoprotein （a-AFP） | 第59-63页 |
| 2．5．1．1 Experimental | 第60-61页 |
| 2．5．1．1．1 Assay procedure for HRP | 第60页 |
| 2．5．1．1．2 Immunoassay | 第60-61页 |
| 2．5．1．2 Results and discussion | 第61-63页 |
| 2．5．1．2．1 Selection of optimal conditions for HRP-catalyzed reaction | 第61页 |
| 2．5．1．2．2 Calibration graph for HRP | 第61页 |
| 2．5．1．2．3 Calibration graph for a-AFP | 第61-62页 |
| 2．5．1．2．4 Immunoassay of AFP in human blood sera | 第62-63页 |
| 2．5．2 A sandwich immunoassay method for the determination of hepatitis B surface antigen （HBsAg） | 第63-65页 |
| 2．5．2．1 Experimental | 第63页 |
| 2．5．2．1．1 Immunoassay for HBsAg | 第63页 |
| 2．5．2．2 Results and disussion | 第63-65页 |
| 2．5．2．2．1 Optimization of catalytic reaction conditions | 第63-64页 |
| 2．5．2．2．2 Calibration graph for HBsAg | 第64页 |
| 2．5．2．2．3 Immunoassay of HBsAg in blood sera | 第64-65页 |
| 2．5．3 Conclusion | 第65页 |
| References | 第65-67页 |
| Chapter 3 Application of reversed micelles as a medium in mimetic peroxidase-catalyzed fluorescence analysis | 第67-84页 |
| 3．1 Introduction | 第67页 |
| 3．2 Materials and apparatus | 第67-68页 |
| 3．2．1 Materials | 第67-68页 |
| 3．2．2 Apparatus | 第68页 |
| 3．3 Study of the effect of AOT reversed micelles on the FeTSPc-catalyzed fluorogenic oxidation reaction of L-tyrosine with H_2O_2 | 第68-74页 |
| 3．3．1 Experimental | 第68-69页 |
| 3．3．1．1 Preparation of the reversed micelle | 第68-69页 |
| 3．3．1．2 Steady-state method | 第69页 |
| 3．3．1．3 Kinetic method | 第69页 |
| 3．3．2 Results and discussion | 第69-74页 |
| 3．3．2．1 Spectral Characteristics | 第69-70页 |
| 3．3．2．2 Influence of AOT reversed m icelles on the fluorogenic reaction | 第70-71页 |
| 3．3．2．3 Optimization of reaction conditions in A OT reversed micelles | 第71-74页 |
| 3．4 Fluorimetric methods of H_2O_2 concentration and FeTSPc activity using an CTAB inverted micellar system | 第74-82页 |
| 3．4．1 Experimental | 第74-75页 |
| 3．4．1．1 Measurement of peroxidase activity of FeTSPc | 第74页 |
| 3．4．1．2 Measurement of fiuorescence intensity with a steady-state method | 第74-75页 |
| 3．4．1．3 The effect of reversed micelles on the fiuorescence of product | 第75页 |
| 3．4．2 Results and discussion | 第75-82页 |
| 3．4．2．1 Spectral characteristics | 第75-79页 |
| 3．4．2．2 Optimum conditions for the FeTSPc-catalyzed reaction in CTAB reversed micelles | 第79-82页 |
| 3．4．2．2．1 Effect of pH | 第79页 |
| 3．4．2．2．2 Reaction time and temperature | 第79-80页 |
| 3．4．2．2．3 Influence of W value on the catalytic reaction | 第80页 |
| 3．4．2．2．4 Influence of CT,4B concentration | 第80-82页 |
| 3．4．2．2．5 The optimum amounts of reagents | 第82页 |
| 3．5 Analytical performance | 第82页 |
| 3．6 Conclusion | 第82-83页 |
| References | 第83-84页 |
| Chapter 4 Determination of nucleic acids based on shifting the association equilibrium of ion-association complex | 第84-105页 |
| 4．1 Introduction | 第84-86页 |
| 4．2 Materials and apparatus | 第86-87页 |
| 4．2．1 Materials | 第86-87页 |
| 4．2．2 Apparatus | 第87页 |
| 4．3 A novel method for the determination of nucleic acids by near infra fluorescence recovery | 第87-94页 |
| 4．3．1 Experimental | 第87页 |
| 4．3．1．1 General procedures | 第87页 |
| 4．3．2 Results and discussion | 第87-94页 |
| 4．3．2．1 Spectral characteristics offluorescence | 第87-88页 |
| 4．3．2．2 Optimization of general procedure | 第88-89页 |
| 4．3．2．2．1 Effect of buffer and pH | 第88-89页 |
| 4．3．2．2．2 Influence of incubation time | 第89页 |
| 4．3．2．2．3 Order of adding reagents | 第89页 |
| 4．3．2．2．4 The optimum amounts of Alcian blue 8GX and HMC | 第89页 |
| 4．3．2．2．5 Effect of salt concentration | 第89页 |
| 4．3．2．3 Exploitation of reaction mechanism | 第89-92页 |
| 4．3．2．3．1 Absorption spectral characteristics of HMC in the presence of Alcian blue 8GX | 第89-90页 |
| 4．3．2．3．2 Absorption spectral characteristics of the Alcian blue 8GX-HMC system in the presence of nucleic acids | 第90-91页 |
| 4．3．2．3．3 Absorption spectral characteristics of Alcian blue 8GX in the presence of nucleic acids | 第91-92页 |
| 4．3．2．4 Tolerance of foreign substances | 第92-93页 |
| 4．3．2．5 Analytical performance | 第93-94页 |
| 4．3．2．6 Applying to sample determination | 第94页 |
| 4．4 Application of FeTSPc-catalyzed fluorescence reaction to the determination of nucleic acids, based on shifting the association equilibrium between FeTSPc and Alcian blue 8GX | 第94-103页 |
| 4．4．1 Experimental | 第94-95页 |
| 4．4．1．1 General procedures | 第94-95页 |
| 4．4．2 Results and discussion | 第95-103页 |
| 4．4．2．1 Spectral characteristics of fluorescence | 第95页 |
| 4．4．2．2 Quenching effect of Alcian blue 8GX on the fluorescence of the FeTSPc-p-HPA-H_2O_2 system | 第95-99页 |
| 4．4．2．3 Fluorescence enhancing effect of nucleic acids on the Alcian blue 8GX-FeTSPc-p-HPA-H_2O_2 system | 第99页 |
| 4．4．2．4 Optimization of experimental parameters | 第99-101页 |
| 4．4．2．4．1 Effect of pH and buffer | 第99-100页 |
| 4．4．2．4．2 Influence of final incubation time | 第100页 |
| 4．4．2．4．3 The optimum amounts of reagents | 第100-101页 |
| 4．4．2．5 Calibration graphs and limits of detection | 第101页 |
| 4．4．2．6 Influence of foreign substances | 第101-102页 |
| 4．4．2．7 Determination of DNA in real sample | 第102-103页 |
| 4．5 Conclusion | 第103页 |
| References | 第103-105页 |
| Chapter 5 Application of the resonance light scattering of phthalocyanines to the determination of biomacromolecules | 第105-121页 |
| 5．1 Introduction | 第105-106页 |
| 5．2 Materials and apparatus | 第106-107页 |
| 5．2．1 Materials | 第106-107页 |
| 5．2．2 Apparatus | 第107页 |
| 5．3 Determination of proteins at nanogram levels by a resonance light-scattering technique with tetra-substituted sulphonated aluminum phthaloeyanine | 第107-114页 |
| 5．3．1 Experimental | 第107页 |
| 5．3．1．1 Procedures | 第107页 |
| 5．3．2 Results and discussion | 第107-113页 |
| 5．3．2．1 Molecluar structure of AlS_4Pc | 第107-108页 |
| 5．3．2．2 Spectral characteristics | 第108-110页 |
| 5．3．2．3 Reaction and mixing sequence | 第110页 |
| 5．3．2．4 Optimization of experimental conditions | 第110-112页 |
| 5．3．2．4．1 Effect of pH and buffer | 第110-111页 |
| 5．3．2．4．2 Optimum amounts of AlS_4Pc | 第111-112页 |
| 5．3．2．5 Interference | 第112页 |
| 5．3．2．6 Calibration graphs and the determination of clinical samples | 第112-113页 |
| 5．3．2．7 Determinations of total protein in serum samples | 第113页 |
| 5．3．3 Conclusions | 第113-114页 |
| 5．4 A new resonance light-scattering method for the determination of nucleic acids at nanogram levels with tetra-substituted amino aluminum phthalocyanine | 第114-119页 |
| 5．4．1 Experimental | 第114页 |
| 5．4．1．1 Standard procedure | 第114页 |
| 5．4．2 Results and Discussion | 第114-119页 |
| 5．4．2．1 Spectral characteristics | 第114-116页 |
| 5．4．2．2 Reaction time and adding sequence of reagents | 第116页 |
| 5．4．2．3 Optimization of the general procedure | 第116-117页 |
| 5．4．2．3．1 Effect of pH and buffers | 第116页 |
| 5．4．2．3．2 Eject of TAAlPc concentration | 第116-117页 |
| 5．4．2．4 Tolerance of foreign substances | 第117-118页 |
| 5．4．2．5 Calibration curves | 第118-119页 |
| 5．4．2．6 Determination of practical sample | 第119页 |
| 5．4．3 Conclusion | 第119页 |
| References | 第119-121页 |
| Acknowledgement in English | 第121-122页 |
| Appendix: Publications & Presentations during Author's Ph．D． Study | 第122-125页 |
