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埃塞俄比亚小麦条锈菌毒性基因遗传分析及小麦材料抗病性鉴定

摘要第7-8页
ABSTRACT第8页
CHAPTER ONE第13-61页
    1.INTRODUCTION第16-19页
    2.LITERATURE REVIEW第19-35页
        2.1.Taxonomy of stripe rust pathogen第19-20页
        2.2.Host range and formae speciales of stripe rust第20-21页
        2.3.Stripe rust races identification and nomenclature using Yr single gene differential system第21-23页
        2.4.Stripe rust Epidemiology第23-24页
        2.5.Stripe rust disease cycle第24页
        2.6.Life cycle of stripe rust(Puccinia striiformis f.sp.tritici)and Puccinia ssp第24-26页
        2.7.Inheritance of virulence/avirulence of cereal rusts pathogenes第26-27页
        2.8.Molecular mapping of cereal rusts fungi genes第27-28页
        2.9.Stripe rust population diversity and utilization of molecular markers第28-30页
            2.9.1.Isozyme and ds RNA Markers第28页
            2.9.2.ITS Markers第28页
            2.9.3.β-Tubulin Gene Markers第28页
            2.9.4.Elongation Factor Markers第28-29页
            2.9.5.RAPD markers第29页
            2.9.6.Restriction fragment length polymorphism(RFLP)第29页
            2.9.7.Amplified fragment length polymorphism(AFLP)第29页
            2.9.8.Ustilization of SSR markers to detected hetrozygousity of stripe rust population第29页
            2.9.9.Utilization of SNP markers to detected hetrozygosity of stripe rust population第29-30页
            2.9.10.Utilization of DNA sequencing to study Pst populations第30页
            2.9.11.Utilization of RNA sequencing to study stripe rust populations第30页
        2.10.Micro evolation mechanisms and dynamic of stripe pathogenic variation第30-33页
            2.10.1.Mutation第30页
            2.10.2.Somatic recombination第30-31页
            2.10.3.Sexual recombination第31页
            2.10.4.Migration and introduction第31-32页
            2.10.5.Genetic drift第32页
            2.10.6.Founder effect第32页
            2.10.7.Host selection第32-33页
            2.10.8.Environmental selection第33页
        2.11.Genome and gunctional genomics of stripe rust pathogen第33页
            2.11.1.Genome of the stripe rust pathogen第33页
            2.11.2.Stripe rust effectors第33页
        2.12.Wheat and Puccinium striiformis interactions第33-34页
        2.13.Genes for stripe rust resistance and histological and cytological defense response in wheat第34-35页
    3.MATERIALS AND METHODS第35-40页
        3.1.Urediniospore and teliospore production of parental isolate第35页
        3.2.Inoculation of barberry leaves with basidiospores第35-36页
        3.3.Development of progeny isolates第36-37页
        3.4.Virulence testing第37页
        3.5.DNA extraction第37-38页
        3.6.Genotyping with SSR markers第38-39页
        3.7.Data analyses第39-40页
    4.RESULTS第40-47页
        4.1.Virulence characterization of the sexual population第40-42页
        4.2.Segregation of progeny isolates第42-43页
        4.3.Linkage analysis and gene mapping第43-47页
    5.DISCUSSION第47-52页
    6.REFERENCE第52-61页
CHAPTER TWO第61-116页
    1.INTRODUCTION第64-67页
    2.LITERATURE REVIEW第67-71页
        2.1.Wheat breeding for stripe rust resistance第67-68页
        2.2.Types of resistance第68-69页
            2.2.1.All stage resistance第68页
            2.2.2.Adult plant resistance第68-69页
            2.2.3.High-temperature adult-plant(HTAP)resistance第69页
        2.3.Resistance type based on degree of resistance第69页
            2.3.1.Complete resistance第69页
            2.3.2.Slow rusting第69页
        2.4.Resistance types based on the genetics of resistance第69-70页
            2.4.1.Monogenic and polygeneic resistance第69页
            2.4.2.Major gene resistance and minor gene resistance第69-70页
            2.4.3.Qualitative and quantitative resistance第70页
            2.4.4.Race specific resistance and non race specific resistance第70页
            2.4.5.Hypersensitive reaction and none hypertensive reaction第70页
        2.5.NBS-LRR resistance and non NBS-LRR resistance第70-71页
    3.MATERIALS AND METHODS第71-81页
        3.1.Plant materials第71-77页
        3.2.Seedling tests第77页
        3.3.Field tests第77页
        3.4.DNA extraction, and PCR Amplification and Electrophoresis第77-81页
    4.RESULTS第81-86页
        4.1.Reactions of the Ethiopian bread wheat cultivars and breeding lines to Pst races第81-83页
        4.2.Detection of Yr resistance genes第83-86页
    5.DISCUSSION第86-89页
    6.REFERENCE第89-93页
    7.SUPPLEMENTARY FILES第93-94页
    8.SUPPLIMENTARY FIGURE第94-115页
    9.ACKNWLEDGEMENTS第115-116页
    10.SELF INTRODUCTION第116页

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