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WRKY转录因子Ⅰ族辣椒CaWRKY58负调控辣椒对青枯菌抗性

Abbreviations/acronyms第7-8页
Abstract第8页
摘要第9-10页
1. Introduction第10-16页
    1.1. The plant immune response第10-11页
    1.2. The plant defense response第11-13页
    1.3. The WRKY superfamily of transcription factors第13-14页
    1.4. Pepper and R.solanacearum第14-15页
    1.5. Objectives of this study第15-16页
2. Materials and methods第16-25页
    2.1. Plant materials and growth conditions第16页
    2.2. Isolation and sequencing of cDNA of CaWRKY58第16-17页
    2.3. Pathogens and Inoculation procedures第17页
    2.4. Application of plant hormones,abiotic and biotic stresses第17-18页
    2.5. Construction of transgenic CaWRKY58 overexpression plants第18页
    2.6. Subcellular Localization第18-19页
    2.7. Co-transfection experiments第19-20页
    2.8. Virus-induced gene silencing (VIGS)of CaWRKY58 in pepper第20页
    2.9. Hsitochemical staining第20-21页
    2.10. Quantitative Real-Time PCR第21-24页
    2.11. Main Primers for PCR used in this study第24-25页
3. Results第25-41页
    3.1 Cloning and sequence analysis of the CaWRKY58第25-26页
    3.2. CaWRKY58 proteins are especially localized to the nuclei第26-27页
    3.3. CaWRKY58 can activate transcription第27-29页
    3.4. Response of CaWRKY58 transcripts to Ralstonia solanacearumnfection第29-30页
    3.5. Transcriptional response of CaWRKY58 to exogenous SA, MeJA,ABA,and ETH application第30-32页
    3.6. Overexpression of CaWRKY58 increased susceptivity to Ralstoniasolanacearum infection in tobacco第32-37页
    3.7. Effect of VIGS of CaWRKY58 on R. solanacearum resistance inpepper第37-41页
4. Discussion第41-45页
5. Literature cited第45-56页
6. Acknowledges第56-57页
7. Publication第57页

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