| Abstract | 第7-9页 |
| 摘要 | 第10-12页 |
| Abbreviations | 第12-14页 |
| Chapter 1:Review of literature | 第14-40页 |
| 1.1 Cotton fiber development | 第14-18页 |
| 1.1.1 Fiber development mechanism | 第14-15页 |
| 1.1.2 Approaches for the improvement of cotton fiber | 第15-18页 |
| 1.2 Factors affecting cotton growth and development | 第18-23页 |
| 1.2.1 Abiotic factors | 第18页 |
| 1.2.2 Biotic factors | 第18-23页 |
| 1.2.2.1 Verticillium wilt | 第19-21页 |
| 1.2.2.2 Fusarium wilt | 第21-23页 |
| 1.3 Thaumatin-like protein(TLP)gene:A member of PR family | 第23-27页 |
| 1.3.1 Role of Pathogenesis-related proteins in plant defense | 第24-27页 |
| 1.4 Functional study of a gene | 第27-30页 |
| 1.4.1 Over-expression | 第27-28页 |
| 1.4.2 RNA silencing | 第28-30页 |
| 1.5 Genetic engineering of commercially important plants | 第30-33页 |
| 1.5.1 Genetic engineering of tobacco | 第30-31页 |
| 1.5.2 Genetic engineering of cotton | 第31-33页 |
| 1.6 Mechanism of Agrobacterium-mediated transformation | 第33-37页 |
| 1.6.1 Agrobacterium:'Natural' genetic engineering | 第35页 |
| 1.6.2 Binary vectors:two from one | 第35-36页 |
| 1.6.3 Steps involved in Agrobacterium-mediated transformation | 第36-37页 |
| 1.7 Aims,strategies and overview of the present study | 第37-40页 |
| 1.7.1 Aims of the study | 第38页 |
| 1.7.2 Approaches/ strategies to accomplish the intended objectives | 第38-39页 |
| 1.7.3 An overview of the present study | 第39-40页 |
| Chapter 2:Materials and methods | 第40-52页 |
| 2.1 Study of GbTLP1 gene in tobacco | 第40-45页 |
| 2.1.1 Vector construction | 第40页 |
| 2.1.2 Transformation and growth of tobacco | 第40-41页 |
| 2.1.3 DNA extraction, PCR and Southern blot analysis | 第41页 |
| 2.1.4 RNA extraction, RT-PCR and Quantitative Realtime PCR analysis | 第41-42页 |
| 2.1.5 Disease resistance evaluation | 第42-43页 |
| 2.1.6 Salt and oxidative stress assay | 第43-44页 |
| 2.1.7 Whole plant drought tolerance test | 第44-45页 |
| 2.1.8 Bioinformatics studies of GbTLP-1 and other proteins of the same family | 第45页 |
| 2.1.9 Statistical analysis | 第45页 |
| 2.2 Study of GbTLP gene in cotton | 第45-52页 |
| 2.2.1 Vector construction | 第45-47页 |
| 2.2.2 Transformation and growth of transgenic cotton | 第47-49页 |
| 2.2.3 DNA extraction and PCR for the selection of positive plants | 第49页 |
| 2.2.4 Standard high volume instrumentation (HVI) measurements | 第49-50页 |
| 2.2.5 Future plan of this study | 第50-52页 |
| 2.2.5.1 Southern blot analysis | 第50页 |
| 2.2.5.2 RNA extraction, RT-PCR and Realtime PCR analysis | 第50页 |
| 2.2.5.3 Disease resistance evaluation | 第50-51页 |
| 2.2.5.4 Fiber cross section to study secondary cell wall development | 第51页 |
| 2.2.5.5 Sub-cellular localization | 第51-52页 |
| Chapter 3:Results | 第52-68页 |
| 3.1 Characteristic features of GbTLP1 | 第52-53页 |
| 3.2 Role of GbTLP1 in secondary cell wall deposition | 第53-54页 |
| 3.3 Results obtained by the transformation of tobacco | 第54-64页 |
| 3.3.1 Transformation and regeneration of transgenic tobacco plants | 第54-55页 |
| 3.3.2 Integration and higher GbTLP1 expression in transgenic tobacco | 第55-57页 |
| 3.3.3 Disease resistance in GbTLP1 transgenic plants | 第57-59页 |
| 3.3.4 Role of GbTLP1 transgenic plants under salinity and oxidative stress | 第59-62页 |
| 3.3.5 Enhanced tolerance of transgenic plants under drought stress | 第62-64页 |
| 3.4 Results obtained by the genetic transformation of cotton | 第64-68页 |
| 3.4.1 Transformation and regeneration of transgenic cotton plants | 第64-66页 |
| 3.4.2 Selection of positive transgenic cotton plants | 第66页 |
| 3.4.3 Standard high volume instrumentation (HVI) measurements of fiber properties | 第66-67页 |
| 3.4.4 Expected results and conclusion | 第67-68页 |
| Chapter 4:Discussion | 第68-71页 |
| Chapter 5:Major findings and future recommendations | 第71-73页 |
| 5.1 Major Findings | 第71-72页 |
| 5.2 Future Recommendations | 第72-73页 |
| References | 第73-95页 |
| Related papers | 第95-96页 |
| Acknowledgements | 第96-98页 |
| CURRICULUM VITAE(C.V.) | 第98-99页 |
