首页--农业科学论文--农作物论文--经济作物论文--棉论文

Functional Analysis of Thaumatin-like Protein Involved in Fiber Secondary Cell Wall Synthesis Through Genetic Transformation of Cotton and Tobacco

Abstract第7-9页
摘要第10-12页
Abbreviations第12-14页
Chapter 1:Review of literature第14-40页
    1.1 Cotton fiber development第14-18页
        1.1.1 Fiber development mechanism第14-15页
        1.1.2 Approaches for the improvement of cotton fiber第15-18页
    1.2 Factors affecting cotton growth and development第18-23页
        1.2.1 Abiotic factors第18页
        1.2.2 Biotic factors第18-23页
            1.2.2.1 Verticillium wilt第19-21页
            1.2.2.2 Fusarium wilt第21-23页
    1.3 Thaumatin-like protein(TLP)gene:A member of PR family第23-27页
        1.3.1 Role of Pathogenesis-related proteins in plant defense第24-27页
    1.4 Functional study of a gene第27-30页
        1.4.1 Over-expression第27-28页
        1.4.2 RNA silencing第28-30页
    1.5 Genetic engineering of commercially important plants第30-33页
        1.5.1 Genetic engineering of tobacco第30-31页
        1.5.2 Genetic engineering of cotton第31-33页
    1.6 Mechanism of Agrobacterium-mediated transformation第33-37页
        1.6.1 Agrobacterium:'Natural' genetic engineering第35页
        1.6.2 Binary vectors:two from one第35-36页
        1.6.3 Steps involved in Agrobacterium-mediated transformation第36-37页
    1.7 Aims,strategies and overview of the present study第37-40页
        1.7.1 Aims of the study第38页
        1.7.2 Approaches/ strategies to accomplish the intended objectives第38-39页
        1.7.3 An overview of the present study第39-40页
Chapter 2:Materials and methods第40-52页
    2.1 Study of GbTLP1 gene in tobacco第40-45页
        2.1.1 Vector construction第40页
        2.1.2 Transformation and growth of tobacco第40-41页
        2.1.3 DNA extraction, PCR and Southern blot analysis第41页
        2.1.4 RNA extraction, RT-PCR and Quantitative Realtime PCR analysis第41-42页
        2.1.5 Disease resistance evaluation第42-43页
        2.1.6 Salt and oxidative stress assay第43-44页
        2.1.7 Whole plant drought tolerance test第44-45页
        2.1.8 Bioinformatics studies of GbTLP-1 and other proteins of the same family第45页
        2.1.9 Statistical analysis第45页
    2.2 Study of GbTLP gene in cotton第45-52页
        2.2.1 Vector construction第45-47页
        2.2.2 Transformation and growth of transgenic cotton第47-49页
        2.2.3 DNA extraction and PCR for the selection of positive plants第49页
        2.2.4 Standard high volume instrumentation (HVI) measurements第49-50页
        2.2.5 Future plan of this study第50-52页
            2.2.5.1 Southern blot analysis第50页
            2.2.5.2 RNA extraction, RT-PCR and Realtime PCR analysis第50页
            2.2.5.3 Disease resistance evaluation第50-51页
            2.2.5.4 Fiber cross section to study secondary cell wall development第51页
            2.2.5.5 Sub-cellular localization第51-52页
Chapter 3:Results第52-68页
    3.1 Characteristic features of GbTLP1第52-53页
    3.2 Role of GbTLP1 in secondary cell wall deposition第53-54页
    3.3 Results obtained by the transformation of tobacco第54-64页
        3.3.1 Transformation and regeneration of transgenic tobacco plants第54-55页
        3.3.2 Integration and higher GbTLP1 expression in transgenic tobacco第55-57页
        3.3.3 Disease resistance in GbTLP1 transgenic plants第57-59页
        3.3.4 Role of GbTLP1 transgenic plants under salinity and oxidative stress第59-62页
        3.3.5 Enhanced tolerance of transgenic plants under drought stress第62-64页
    3.4 Results obtained by the genetic transformation of cotton第64-68页
        3.4.1 Transformation and regeneration of transgenic cotton plants第64-66页
        3.4.2 Selection of positive transgenic cotton plants第66页
        3.4.3 Standard high volume instrumentation (HVI) measurements of fiber properties第66-67页
        3.4.4 Expected results and conclusion第67-68页
Chapter 4:Discussion第68-71页
Chapter 5:Major findings and future recommendations第71-73页
    5.1 Major Findings第71-72页
    5.2 Future Recommendations第72-73页
References第73-95页
Related papers第95-96页
Acknowledgements第96-98页
CURRICULUM VITAE(C.V.)第98-99页

论文共99页,点击 下载论文
上一篇:The Formation Mechanism and the Suppression Technology Study of Multiple Wave of the South Yellow Sea Seismic Data
下一篇:The Anti-aging Effect of Tai Chi on Neuromuscular System Among Elderly