| LIST OF FIGURES | 第9-10页 |
| ABBREVIATIONS | 第10-11页 |
| ABSTRACT(IN ENGLISH) | 第11页 |
| ABSTRACT(IN CHINESE) | 第12-13页 |
| 1.INTRODUCTION | 第13-20页 |
| 2.MATERIALS AND METHODS | 第20-32页 |
| 2.1 Materials | 第20-23页 |
| 2.1.1 Insects and collection of hemolymph | 第20页 |
| 2.1.2 Reagents and buffers | 第20-23页 |
| 2.2 Measurement of amidase activity | 第23-24页 |
| 2.3 Determination of melanin synthesis | 第24页 |
| 2.4 Amino acid sequencing | 第24页 |
| 2.5 Antibodies and Western blot | 第24页 |
| 2.6 Purification of Tm-proPO,Tm-SPE and Tm-SPH2 zymogen | 第24-30页 |
| 2.6.1 Preparation of E1,E2 and E3 fractions by using Toyopearl AF-Heparin HC column | 第24-26页 |
| 2.6.2 Purification of Tm-pro-PO protein from E2 fraction | 第26页 |
| 2.6.3 Purification of zymogenic and active form of 44 kDa proteins from E2 fraction | 第26-28页 |
| 2.6.3.1 Purification of zymogenic form of SPE | 第27页 |
| 2.6.3.2 Purification of active form of SPE | 第27-28页 |
| 2.6.4 Purification of Tm-SPH 1 protein | 第28-29页 |
| 2.6.5 Purification of Tm-SPH 2 protein from Toyopearl AF-Heparin HC column flow-through fraction | 第29-30页 |
| 2.7 Injection of protein into Tenebrio Molitor larvae | 第30-31页 |
| 2.8 Assay of antibacterial activity | 第31-32页 |
| 3.RESULTS | 第32-41页 |
| 3.1 The injection of active form of Tm-SPE to Tenebrio larvae induced melanin synthesis in vivo | 第32-33页 |
| 3.2 Tm-SPE can cleave Tm-proPO into three fractions | 第33-35页 |
| 3.3 Tm-SPE specifically cleaved Tm-SPH1 but not Tm-SPH2 | 第35-36页 |
| 3.4 Chemically cross-linked adducts are generated by the melanization complex | 第36-39页 |
| 3.5 Locally concentrated melanin on the surface of bacteria shows bactericidal effect | 第39-41页 |
| 4.DISCUSSION | 第41-44页 |
| 5.REFERENCES | 第44-47页 |
| 6.ACKNOWLEDGEMENT | 第47页 |
