| 摘要 | 第4-6页 |
| ABSTRACT | 第6-7页 |
| 1 INTRODUCTION | 第10-25页 |
| 1.1 Nuclear Factor Y(NF-Y) | 第10-20页 |
| 1.1.1 Nuclear factor Y and CCAAT element | 第10页 |
| 1.1.2 NF-Y family in plant | 第10-11页 |
| 1.1.3 Molecular characterizations of NF-Y in plant | 第11-13页 |
| 1.1.4 Roles of NF-Y in plant growth and development | 第13-17页 |
| 1.1.5 NF-Ys in environmental stress response | 第17-20页 |
| 1.2 Potato | 第20-23页 |
| 1.2.1 Potato production | 第20页 |
| 1.2.2 Nutritional characteristics of potatoes | 第20-21页 |
| 1.2.3 Potato breeding | 第21-22页 |
| 1.2.4 Molecular markers and breeding in potato | 第22-23页 |
| 1.3 The present study | 第23-25页 |
| 2 MATERIALS AND METHODS | 第25-36页 |
| 2.1 Identification of potato NF-YB subunits | 第25页 |
| 2.2 Alignment, phylogenetic and gene structure analysis | 第25页 |
| 2.3 RNA-seq data analysis | 第25-27页 |
| 2.4 Plant materials and growth condition | 第27-28页 |
| 2.5 cDNA cloning | 第28页 |
| 2.6 Vector construction and generation of transgenic plants | 第28-29页 |
| 2.7 Quantitative real time PCR (qRT PCR) | 第29-32页 |
| 2.8 Field design and evalution | 第32-33页 |
| 2.9 Relative water content (RWC) | 第33-34页 |
| 2.10 Drought tolerance assessment | 第34页 |
| 2.11 Guard cell and stomatal bioassays | 第34-35页 |
| 2.12 Statistical analysis | 第35-36页 |
| 3 RESULTS | 第36-84页 |
| 3.1 Identification of potato NF-YBs | 第36-44页 |
| 3.1.1 Phylogenetic tree and alignment analysis | 第36页 |
| 3.1.2 Chromosomal distribution gene duplication analysis | 第36-40页 |
| 3.1.3 Gene structure analysis | 第40-41页 |
| 3.1.4 Expression profiles of NF-YB | 第41-44页 |
| 3.2 StNF-YB3.1 was involved in photosynthesis and tuber yield | 第44-83页 |
| 3.2.1 Homologue analysis of StNF-YB3.1 | 第44-59页 |
| 3.2.2 Transcription of StNF-YB3.1 in potato organs | 第59-60页 |
| 3.2.3 Development of StNF-YB3.1 overexpression lines | 第60-63页 |
| 3.2.4 Phenotypic analysis of OEYB3.1 lines | 第63-68页 |
| 3.2.5 Photosynthetic capacity of OEYB3.1 lines | 第68-71页 |
| 3.2.6 Stomatal closure of OEYB3.1 lines in response to ABA | 第71页 |
| 3.2.7 Expression of genes involved in ABA-mediated stomatal closure | 第71-79页 |
| 3.2.8 Evaluation of drought tolerance of OEYB3.1 lines | 第79-83页 |
| 3.3 Conclusion | 第83-84页 |
| 4 DISCUSSION | 第84-91页 |
| 4.1 Structural analysis and expression characteristics of StNF-YBs | 第84-85页 |
| 4.2 StNF-YB3.1 involved in potato flowering | 第85-86页 |
| 4.3 StNF-YB3.1 involved in tuber production and photosynthesis | 第86页 |
| 4.4 Overexpression of StNF-YB3.1 resulted higher chlorophyll content | 第86-87页 |
| 4.5 Overexpression of StNF-YB3.1 accelerated stomatal closure | 第87-88页 |
| 4.6 Overexpression of StNF-YB3.1 up-regulated genes for ABA signaling | 第88-89页 |
| 4.7 StNF-YB3.1 involved in abiotic stress | 第89页 |
| 4.8 OEYB 3.1 exhibited lower RWC under drought treatments | 第89-91页 |
| REFERENCE | 第91-109页 |
| ACKNOWLEDGEMENT | 第109页 |
| PUBLICATION | 第109页 |
