| ABSTRACT | 第1-7页 |
| 摘要 | 第7-11页 |
| Chapter 1 Introdtiction | 第11-25页 |
| l.l Endosperm development and imprinting | 第11-16页 |
| ·Endosperm development | 第11-12页 |
| ·Genomic imprinting and seed development | 第12-16页 |
| ·Discovery of genomic imprinting and its evolution | 第12-13页 |
| ·Occurrence and erase of imprinting | 第13-15页 |
| ·Imprinted genes and their function | 第15-16页 |
| ·Epigenetic mechanisms of imprinting | 第16-23页 |
| ·DNA methylation | 第17-21页 |
| ·DNA methylation and its maintenance | 第17-18页 |
| ·Function of DNA methylation | 第18-21页 |
| ·Histone Modification | 第21-23页 |
| ·Histone methylation | 第21-23页 |
| ·Aims | 第23-25页 |
| Chapter 2 Materials and Methods | 第25-29页 |
| ·RNA and DNA isolation from rice endosperm | 第25页 |
| ·RT-PCR and sequencing | 第25-26页 |
| ·Bisulfite sequencing | 第26页 |
| ·5'RACE at Loc_Os11g36470 and Loc_os01g69110 | 第26-27页 |
| ·Chromatin immunoprecipitation(ChIP)assay using antibody against H3K27me_3 | 第27-28页 |
| ·Germinating Nip seeds on 5-azacytidine and zebularine solutions | 第28-29页 |
| Chapter 3 Results | 第29-46页 |
| ·Maternal hypomethylation and H3K27me3 together with paternal allele methylation are associated with paternal expression of OsYUCCA11 | 第29-35页 |
| ·Differential DNA methylation in the promoter region of maternal and paternal alleles of Loc_Os01g69110 results in alternative transcript initiation | 第35-40页 |
| ·DNA hypomethylation in the gene body of Os11g36470 contributes to production of shorter maternally biased transcripts in the endosperm | 第40-46页 |
| Chapter 4 Discussion | 第46-51页 |
| ·The conserved imprinted YUCCA genes in rice and Arabidopsis are regulated by similar mechanisms | 第46-48页 |
| ·Parent of origin specific activation of cryptic promoters requires DNA demethylation | 第48-51页 |
| REFERENCES | 第51-59页 |
| Supplementary Figures | 第59-68页 |
| Supplementary Tables | 第68-72页 |
| Publication | 第72-73页 |
| Acknowledgement | 第73页 |
