| Abstract | 第6-7页 |
| Abbreviations | 第8-10页 |
| Introduction | 第10-12页 |
| Chapter 1:Review of literature | 第12-26页 |
| 1.1 Abiotic stresses | 第12页 |
| 1.2 Basic theory of stress | 第12-13页 |
| 1.3 Drought stress | 第13-14页 |
| 1.4 Salt stress | 第14-15页 |
| 1.5 High temperature stress | 第15-18页 |
| 1.6 Low temperature stress | 第18-19页 |
| 1.7 Signal transduction mechanism during low temperature stress | 第19-22页 |
| 1.7.1 Role of calcium during signal transduction | 第20页 |
| 1.7.2 Role of ABA during signal transduction | 第20-21页 |
| 1.7.3 Role of solute accumulation during signal transduction | 第21-22页 |
| 1.8 Tolerance mechanism in plants during cold stress | 第22-24页 |
| 1.8.1 Metabolic modification provides cold tolerance | 第22-23页 |
| 1.8.2 Role of antioxidants enzymes during cold stress | 第23页 |
| 1.8.3 Function of cold stress proteins during cold tolerance mechanism | 第23-24页 |
| 1.9 Aim of the study | 第24-26页 |
| Chapter 2:Materials and Methods | 第26-31页 |
| 2.1 Plant cultivation and treatment | 第26页 |
| 2.2 Identification of full-length cDNA and qRT-PCR analysis | 第26页 |
| 2.3 Vector construction, yeast transformation and characterization by northern blot | 第26-27页 |
| 2.4 Construction of plant expression cassettes and tobacco transformation | 第27-28页 |
| 2.5 Germination and stress experiments in tobacco | 第28页 |
| 2.6 Stress-responsive gene expression analysis by RT-PCR | 第28-29页 |
| 2.7 Measurements of morphological and physiological parameters | 第29页 |
| 2.8 Cold tolerance assay of GhCAX1 by using NaN_3 | 第29页 |
| 2.9 Oxidative stress tolerance assay of GhCAX1 | 第29-31页 |
| Chapter 3:Results | 第31-48页 |
| 3.1 Expression analysis of GhCAX3 and GhCAXl under ABA treatment | 第31-32页 |
| 3.2 Over-expression of GhCAX3 in tobacco alleviates ABA sensitivity | 第32-34页 |
| 3.3 Over-expression of GhCAX3 confers cold sensitivity on seed germination and tolerance on seedlings, respectively | 第34-36页 |
| 3.4 Involving of GhCAX3 in regulation between ABA and cold signal transduction | 第36-38页 |
| 3.5 Overexpression of GhCAX1 in tobacco alleviates ABA and salt sensitivity | 第38-40页 |
| 3.6 Overexpression of GhCAX1 confers cold tolerance on Seedlings development and mature plants | 第40-43页 |
| 3.7 POD inhibition compromises the freezing tolerance of transgenic tobacco | 第43-46页 |
| 3.8 GhCAX1-Overexpressing Plants enhance Oxidative Stress Tolerance | 第46-48页 |
| Chapter 4: Discussion | 第48-54页 |
| CONCLUSION | 第54-55页 |
| References | 第55-70页 |
| Related papers | 第70-71页 |
| 附件 | 第71-72页 |
| Acknowledgement | 第72-73页 |
