| 摘要 | 第10-11页 |
| ABSTRACT | 第11页 |
| 1. General Introduction | 第12-31页 |
| 1.1 CTLs in insect's innate immunity | 第15-19页 |
| 1.1.1 CTLs domain organization and structure | 第15-16页 |
| 1.1.2 Phylogenetic analysis of CTLs from several insects | 第16-17页 |
| 1.1.3 C-type lectins in various Insects | 第17-19页 |
| 1.2 Innate immunity in insects | 第19-22页 |
| 1.2.1 Cellular immune responses in insects | 第19-20页 |
| 1.2.2 Humoral response in insect | 第20-22页 |
| 1.3 Pattern recognition receptors (PRRs) | 第22-25页 |
| 1.3.1 Peptidoglycan recognition binding proteins (PGRPs) | 第22-24页 |
| 1.3.2 Gram-negative binding proteins (GNBPs) | 第24-25页 |
| 1.3.3 Hemolin | 第25页 |
| 1.3.4 Integrin's | 第25页 |
| 1.4 Signaling pathways in innate immunity | 第25-30页 |
| 1.4.1 Toll Pathway in Drosophila melanogaster | 第25-27页 |
| 1.4.2 The immune deficiency (IMD) pathway in Drosophila melanogaster | 第27-29页 |
| 1.4.3 JAK/STAT pathway | 第29-30页 |
| 1.4.4 RNA interference pathway | 第30页 |
| 1.5 Purpose and significance of this study | 第30-31页 |
| 2. Materials and Methods | 第31-42页 |
| 2.1 Insect collection and rearing | 第31页 |
| 2.2 RNA extraction, gel electrophoresis, and cDNA synthesis | 第31-32页 |
| 2.3 Molecular characterization of isolated template | 第32-33页 |
| 2.3.1 Cloning of the target fragment | 第33页 |
| 2.4 Rapid Amplification of 3 'End Sequence of RfCTL (3' RACE) | 第33-35页 |
| 2.4.1 Synthesis of 3 'RACE cDNA | 第33-34页 |
| 2.4.2 3'RACE sequence amplification | 第34页 |
| 2.4.3 Recovery of PCR product and cloning | 第34-35页 |
| 2.5 Rapid Amplification of 5 'End Sequence of RfCTL (5'RACE) | 第35-36页 |
| 2.5.1 5'RACE sequence amplification | 第35-36页 |
| 2.6 Molecular characterization and sequence analysis of RfCTL | 第36页 |
| 2.7 Phylogenetic analysis | 第36页 |
| 2.8 Quantitative real-time PCR(qRT-PCR)analysis of the expression profiles of RfCTL indifferent tissues | 第36-37页 |
| 2.9 Evaluation of RfCTL in relation to the bacterial challenge | 第37-38页 |
| 2.10 RNA interference | 第38-42页 |
| 2.10.1 Primer design with T7 promoter | 第38页 |
| 2.10.2 Preparation of dsRNA template | 第38页 |
| 2.10.3 Preparation of dsRNA | 第38-40页 |
| 2.10.4 dsRNA microinjection | 第40-42页 |
| 3. Results | 第42-53页 |
| 3.1 Characterization and features of RfCTL | 第42-46页 |
| 3.2 Phylogenetic analysis of RfCTL with various known CTLs | 第46-48页 |
| 3.3 Expression profile of RfCTL across different tissues | 第48-49页 |
| 3.4 RfCTL expression is induced by bacterial challenge | 第49-52页 |
| 3.5 Verification of RNAi effects on the RfCTL | 第52-53页 |
| 4. Discussion | 第53-56页 |
| Conclusion | 第56-57页 |
| References | 第57-74页 |
| Acknowledgement | 第74-75页 |
