| 摘要 | 第3-6页 |
| Abstract | 第6-9页 |
| List of abbreviations | 第12-13页 |
| Chapter 1 Introduction | 第13-35页 |
| 1.1 The current study of pair bonding | 第13-14页 |
| 1.2 The development of early social environment influences on the individual's offspring | 第14-15页 |
| 1.3 The effect of paternal care on offspring pair bonding at adult | 第15-17页 |
| 1.4 Arginine vasopressin (AVP), Arginine vasopressin 1a receptor (V1aR) and pair bonding | 第17-20页 |
| 1.5 Vasopressin, Ventral Pallidum, Lateral septum and Pair Bonding | 第20-22页 |
| 1.6 The current status of methylation | 第22-24页 |
| 1.7 The epigenetic mechanisms underlying social behavior | 第24-28页 |
| 1.8 Mandarin vole:potential animal models for studying pair bonding in, monogamous species | 第28-30页 |
| 1.9 The basis of our study | 第30-32页 |
| 1.10 The significance and content of our study | 第32-35页 |
| Chapter 2 Materials and methods | 第35-45页 |
| 2.1 Experiment 1 | 第35-41页 |
| 2.1.1 Subjects | 第35页 |
| 2.1.2 Treatment groups | 第35页 |
| 2.1.3 LS and VP tissue collection | 第35-36页 |
| 2.1.4 DNA and RNA extraction | 第36-38页 |
| 2.1.5 Bisulfite Modification | 第38-40页 |
| 2.1.6 Real-time quantitative polymerase chain reaction analysis | 第40-41页 |
| 2.1.7 Statistical analyses | 第41页 |
| 2.2 Experiment 2 | 第41-45页 |
| 2.2.1 Animals and treatment | 第42页 |
| 2.2.2 Behavioral testing | 第42-44页 |
| 2.2.3 Statistical analyses | 第44-45页 |
| Chapter 3 Results | 第45-53页 |
| 3.1 whether paternal care or not alter the DNA methylation patterns of some CpG sites within the V1aR promoter region in the LS and VP | 第45-46页 |
| 3.2 The effect of V1aR-VP on social interaction and pair bonding in male voles Figures and captions | 第46-53页 |
| Chapter 4 Discussion | 第53-65页 |
| 4.1 Effects of paternal deprivation on levels of V1aR mRNA expression and DNA methylation | 第53-62页 |
| 4.2 Effects of the over-expression of V1aR-VP on pair bonding and social behavior | 第62-65页 |
| Chapter 5 Future direction | 第65-67页 |
| References | 第67-81页 |
| Acknowledgements | 第81-83页 |
| List of Publication | 第83页 |
