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DNL2与AB-1,两种植物生长发育相关基因在水稻中的精细定位和特征

中文摘要第4-5页
English abstract第5页
List of abbreviations and acronyms第9-10页
1 PARTⅠ:REVIEW第10-13页
    1.1 Riceproduction第10页
    1.2 Plant growth and development第10-13页
        1.2.1 Vegetative growth stage第11页
        1.2.2 Leaf development第11-13页
2 PARTⅡ:CHARACTERIZATION AND FINE MAPPING OF A DWARF AND NARROWLEAF MUTANT GENE DNL2 IN RICE第13-25页
    2.1 Introduction第13-15页
    2.2 Materials and methods第15-16页
        2.2.1 Plant materials and field experimentation第15页
        2.2.2 Paraffin sectioning and histological analysis第15页
        2.2.3 Gibberellic acid 3(GA3) and brassinolide(BL)induction of shoot elongation第15页
        2.2.4 Determination of crude fiber content第15-16页
        2.2.5 RNA extraction and quantitative real time RT-qPCR第16页
        2.2.6 Fine mapping第16页
    2.3 Results第16-23页
        2.3.1 The dnl2 mutation affects plant height,plant architecture and grain yield第16-18页
        2.3.2 Physical and biochemical characterization of the mutant's leaf and stem第18-20页
        2.3.3 The mutant's leaf and stem were brittle第20-21页
        2.3.4 Fine mapping and candidate genes analysis of dnl2 mutant genes第21-23页
    2.4 Discussion第23-25页
3 PARTⅢ:FURTHER INSIGHT INTO THE ROLE OF ABAXIALIZED-1,AN ALLELICMUTANT OF KANADI FAMILY MEMBER KAN1 IN RICE第25-46页
    3.1 Introduction第25-27页
    3.2 Materials and Methods第27-30页
        3.2.1 Plant materials and field experiments第27页
        3.2.2 Paraffin sectioning and histological analysis of leaves第27页
        3.2.3 Scanning electron microscopic observation of plant culms第27页
        3.2.4 Exogenous application NAA,IAA),and GA3第27-28页
        3.2.5 Hormone content第28页
        3.2.6 RNA extraction and real time quantitative PCR(qPCR)第28页
        3.2.7 Fine mapping第28页
        3.2.8 Plasmid construction第28-29页
        3.2.9 Plant transformation第29页
        3.2.10 Phylogenetic and protein structure analysis第29页
        3.2.11 Yeast two-hybrid screening analysis第29-30页
    3.3 Results第30-43页
        3.3.1 The ab-1 showed several morphological defects第30-31页
        3.3.2 The ab-1 has an altered bulliform cells in adaxial side of the leaves第31-32页
        3.3.3 The KANADI1 transcription factor is candidate gene of ab-1第32-33页
        3.3.4 The AB-1 and ARF3 displayed an overlapping expression pattern第33-34页
        3.3.5 Protein structure and phylogenetic analysis of AB-1第34页
        3.3.6 The AB-1 and its homologs involved in leaf polarity determination in rice第34-39页
        3.3.7 The AB-1 might physically interact with ARFs proteins in rice第39-40页
        3.3.8 The AB-1 is involved in IAA and GA homoeostasis in rice第40-42页
        3.3.9 The ab-1 affected expression of putative genes involved in leaf development第42-43页
    3.4 Discussion第43-45页
    3.5 General conclusion第45-46页
Acknowledgement第46页
References第46-53页
Supplemental data第53-59页
个人简历第59页
博士期间发表的论文第59页
博士后期间发表的论文第59-60页
永久通信地址第60页

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