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Preliminary Genetic Studies on Resistance to Powdery Mildew in Cucumber

Abstract第4-5页
摘要第6-8页
Symbols第8-9页
Chapter 1:General introduction第9-31页
    1.1 General life cycle of powdery mildews第12-14页
    1.2 Effect of temperature on the development of powdery mildews第14-15页
    1.3 Inheritance of powdery mildew resistance and relation to temperature第15-26页
    1.4 References第26-31页
Chapter 2:Literature review第31-76页
    2.1 Markers, populations and map construction第31-38页
        2.1.1 Genetic markers第31-36页
        2.1.2 Mapping population第36-37页
        2.1.3 Map construction第37-38页
    2.2 Statistical methods for QTL mapping第38-42页
        2.2.1 Single marker analysis第39页
        2.2.2 Interval mapping第39-41页
        2.2.3 Composite interval mapping第41页
        2.2.4 Multiple interval mapping第41-42页
    2.3 Epistatic and environmental interactions for quantitative trait loci第42页
    2.4 QTL mapping in Cucubitaceae species第42-44页
        2.4.1 QTL mapping and linkage analyses in Cucumber第43-44页
    2.5 Plant defence signalling networks第44-54页
        2.5.1 Mitogen activated protein kinases第44-46页
        2.5.2 Calcium dependent protein kinases第46-47页
        2.5.3 Phospholipid signalling第47页
        2.5.4 Reactive oxygen species and nitric oxide第47-50页
        2.5.5 Salicylic acid and systemic acquired resistance第50-52页
        2.5.6 Jasmonic acid and ethylene第52-54页
        2.5.7 Cross talk between signalling pathways第54页
    2.6 Biochemistry of Plant Defence Responses第54-61页
        2.6.1 Pathogenesis related proteins第55-56页
        2.6.2 Cell wall fortification第56-57页
        2.6.3 Lipoxygenases第57-58页
        2.6.4 Phytoalexins第58页
        2.6.5 Transcription factors第58-61页
    2.7 References第61-76页
Chapter 3:SCAR marker linked to powdery mildew resistance for selection of near-isogenc lines in cucumber第76-89页
    3.1 Introduction第76-77页
    3.2 Materials and methods第77-80页
        3.2.1 Plant materials第77页
        3.2.2 Disease resistance test第77-78页
        3.2.3 Marker analysis第78-79页
        3.2.4 Screening and markers verifying第79页
        3.2.5 Statistical analysis第79页
        3.2.6 Excision and cloning procedures第79-80页
        3.2.7 Selection of resistant NILs using SCAR markers第80页
    3.3 Results第80-85页
        3.3.1 Markers screening第80-81页
        3.3.2 SCAR designing and sequencing第81-82页
        3.3.3 Analysis of SCAR and resistance gene linkage第82-84页
        3.3.4 MAS in resistant NILs using SCAR markers第84-85页
    3.4 Discussion第85-86页
    3.5 Conclusion第86-87页
    3.6 References第87-89页
Chapter 4:Mapping quantitative trait loci for powdery mildew resistance in cucumber using SRAP and ISSR markers第89-102页
    4.1 Introduction第89-90页
    4.2 Materials and methods第90-93页
        4.2.1 Plant materials第90-91页
        4.2.2 Disease resistance test第91页
        4.2.3 DNA extraction and molecular marker analysis第91-93页
        4.2.4 QTL analysis第93页
    4.3 Results第93-97页
        4.3.1 Powdery mildew investigation第93-94页
        4.3.2 ISSR and SRAP markers analyses第94-95页
        4.3.3 Linkage map and marker segregation第95-96页
        4.3.4 QTL analysis第96-97页
    4.4 Discussion第97-98页
    4.5 Conclusion第98页
    4.6 References第98-102页
Chapter 5:Expression of WRKY and MYB Genes during infection with powdery mildew in cucumber第102-112页
    5.1 Introduction第102-104页
    5.2 Materials and methods第104-105页
        5.2.1 Plant and fungal materials第104页
        5.2.2 RNA isolation and reverse transcription-PCR第104-105页
        5.2.3 Quantitative real-time PCR and gene expression analysis第105页
    5.3 Results第105-107页
    5.4 Discussion第107-109页
    5.5 Conclusion第109页
    5.6 References第109-112页
6. Conclusion第112-113页
7. Acknowledgement第113-114页
Publications第114-115页

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