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CLONING AND SEQUENCE ANALYSIS OF TWO SATELLITE RNAs FROM CUCUMBER MOSAIC VIRUS P1 ISOLATE

Declaration.第4-5页
Certification.第5-6页
Dedication.第6-7页
Acknowledge第7-9页
List of figures and tables.第9-10页
Abbreviations.第10-12页
Unit abbreviations.第12-16页
Abstract.第16页
Chapter 1: Introduction and literature review.第17-49页
    Introduction.第17-19页
    Literature Review.第19-49页
        General aspects about virus.第19-26页
            Definition of virus.第19页
            Virus replication.第19-22页
                Replication strategy.第19-20页
                Replication of RNA virus.第20-21页
                    Positive strand RNA viruses.第20页
                    Negative strand RNA viruses.第20-21页
                    Double-stranded RNA viruses.第21页
                Replication of DNA viruses.第21-22页
                    Caulimoviruses.第21页
                    Geminiviruses.第21-22页
            Virus transmission.第22-25页
                Direct passage in living higher plant material.第22-23页
                    Through the seed and pollen.第22-23页
                    By vegetative propagation.第23页
                    By grafting.第23页
                Transmission by organisms other than higher plant.第23-24页
                    Invertebrates.第23页
                    Fungi.第23-24页
                    Nematode.第24页
                    Experimental transmission by Agrobacterium tumefaciens.第24页
                Mechanical transmission.第24-25页
            Plant virus disease control.第25-26页
        Cucumber mosaic virus.第26-37页
            Taxonomy.第26-27页
            Symptomatology and pathology.第27-29页
            Genomic structure.第29-30页
            Replication, entry and disassembly.第30-32页
                Formation of viral replication complex.第30页
                Viral RNA replication.第30-31页
                Expressions of ”late” viral genes.第31页
                Encapsidation.第31-32页
                Movement.第32页
            Transmission.第32-33页
            Induction of diseases.第33-35页
            Transgenic plants.第35-37页
        Satellites.第37-49页
            Satellite viruses and satellite RNAs.第37-40页
                Satellite plant viruses.第38页
                Satellite RNAs.第38-40页
                    Satellite RNAs replication via a negative sense monomer template.第38-39页
                    Satellite RNAs with a viroidlike replication.第39-40页
                    Symptom modulation by satellite RNAs.第40页
            Satellite RNAs of plant viruses: Structures and biological effects.第40-49页
                Nucleotide sequence and structure.第41-43页
                    Potentia1 ORFs.第41页
                    Secondary structure.第41-42页
                    Sequence variation.第42-43页
                Replication.第43-44页
                    Specificity and efficiency.第43页
                    Self-processing.第43-44页
                Effect on helper virus.第44-47页
                    Alteration in virus titer.第44页
                    Symptom modulation.第44-46页
                    Strategies for virus control.第46-47页
                Sate1litelike molecules.第47-49页
                    Origins.第47-48页
                    Conclusions.第48-49页
Chapter 2: Materials and Methods.第49-55页
    Virus strain and inoculation.第49页
    Double-strand RNA (dsRNA) purification.第49-50页
    Denaturing dsRNA.第50页
    cDNA synthesis.第50页
    Polymerase Chain Reaction (PCR).第50-51页
    PCR product purification, cloning and sequencing.第51-55页
        PCR product purification.第51页
        Ligation.第51-52页
        Transformation.第52-53页
        Plasmid extraction by Boiling Method.第53-54页
        Sequencing determination and analysis.第54-55页
Chapter 3: Results.第55-65页
    Biology of CMVP1.第55页
    Double-strand RNA (dsANA) purification.第55-56页
    cDNA synthesis and Polymerase Chain Reaction (PCR).第56-57页
    Cloning and Screening.第57页
    Sequence.第57-65页
        Coding capacity of the satellite RNAs.第61页
        Secondary structure analysis of CMVP1 sat-1,2.第61-65页
Chapter 4: Discussion and conclusions.第65-68页
    Discussion.第65-67页
    Conclusions.第67-68页
References.第68-73页

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