| 答辩委员会签名表、博士学位论文评阅人 | 第5-6页 |
| 摘要 | 第6-8页 |
| Abstract | 第8-10页 |
| Abbreviation | 第15-16页 |
| Chapter Ⅰ:General Introduction | 第16-27页 |
| 1.1 History and origin of garlic | 第16页 |
| 1.2 Garlic production | 第16-18页 |
| 1.3 Medicinal properties | 第18-19页 |
| 1.4 Garlic breeding | 第19页 |
| 1.5 Genetic diversity | 第19-23页 |
| 1.5.1 Phenotype | 第20页 |
| 1.5.2 Biochemical analysis | 第20-22页 |
| 1.5.3 Molecular markers | 第22-23页 |
| 1.6 Mutation breeding | 第23-25页 |
| 1.6.1 Physical mutagens | 第23-24页 |
| 1.6.2 Chemical mutagens | 第24-25页 |
| 1.7 Significance of the Research | 第25-26页 |
| 1.8 Statement of the problem and objectives | 第26-27页 |
| Chapter Ⅱ:Impact of Genetic Factor and Environment(Geographical Location)onAllicin Content of Garlic(Allium sativum L.) Germplasm from Egypt and China | 第27-39页 |
| 2.1 Abstract | 第27页 |
| 2.2 Introduction | 第27-28页 |
| 2.3 Materials and methods | 第28-32页 |
| 2.3.1 Plant Material | 第28-30页 |
| 2.3.2 Experimental Design | 第30页 |
| 2.3.3 Sample Preparation | 第30-31页 |
| 2.3.4 Reagents and Chemicals | 第31页 |
| 2.3.5 Standard Preparation | 第31页 |
| 2.3.6 Method Validation | 第31页 |
| 2.3.7 Allicin Determination | 第31-32页 |
| 2.3.8 Data analysis | 第32页 |
| 2.4 Results | 第32-37页 |
| 2.4.1 Genetic variation of allicin contents among garlic germplasm of different origins | 第32-35页 |
| 2.4.2 The effect of genetic improvement on allicin contents by single plant selection and bulk selection | 第35-36页 |
| 2.4.3 Combined Influence of genotypes and geographic location on allicin content in Egyptian garlic germplasms | 第36-37页 |
| 2.5 Discussion | 第37-39页 |
| Chapter Ⅲ:Estimation of genetic diversity among garlic germplasm based onmorphological traits and new microsatellite markers | 第39-53页 |
| 3.1 Abstract | 第39页 |
| 3.2 Introduction | 第39-40页 |
| 3.3 Materials and methods | 第40-44页 |
| 3.3.1 Plant material | 第40-42页 |
| 3.3.2 Morphological characteristics | 第42页 |
| 3.3.3 SSR marker devlopment | 第42页 |
| 3.3.4 DNA extraction | 第42-43页 |
| 3.3.5 DNA fragment amplification | 第43-44页 |
| 3.3.6 PCR product separation and gel staining | 第44页 |
| 3.3.7 Data analysis | 第44页 |
| 3.4 Result | 第44-50页 |
| 3.4.1 Morphological diversity | 第44-46页 |
| 3.4.2 SSR polymorphism | 第46-49页 |
| 3.4.3 Genetic diversity analysis of 104 garlic germplasms from China and Egypt based on SSR markers | 第49-50页 |
| 3.4.4 Effects of genetic improvements through clonal selection | 第50页 |
| 3.5 Discussions | 第50-53页 |
| Chapter Ⅳ:Sprout differentiation and mutation induction of garlic (Allium sativumL.) callus exposed to gamma rays | 第53-71页 |
| 4.1 Abstract | 第53页 |
| 4.2 Introduction | 第53-54页 |
| 4.3 Materials and methods | 第54-58页 |
| 4.3.1 Plant materials | 第54-55页 |
| 4.3.2 Initiation of callus | 第55页 |
| 4.3.3 Physical mutation treatment by Gamma ray(γ) | 第55页 |
| 4.3.4 Sprout and root induction | 第55-56页 |
| 4.3.5 Acclimatization | 第56-57页 |
| 4.3.6 Identify the mutant using SSR marker | 第57-58页 |
| 4.3.7 Data collection and statistical analysis | 第58页 |
| 4.4 Results | 第58-68页 |
| 4.4.1 Influence of gamma rays treatments on the survival number of callus | 第58-59页 |
| 4.4.2 Influence of gamma rays treatments in days needed for bud emergence | 第59-60页 |
| 4.4.3 Influence of gamma rays treatments in days for callus to continue producing sprouts | 第60页 |
| 4.4.4 Influence of gamma rays tre:atments on average sprout number per callus | 第60-61页 |
| 4.4.5 Influence of gamma rays treatments on rate of sprouts induction | 第61-62页 |
| 4.4.6 Influence of gamma rays treatments on total number of sprouts | 第62-63页 |
| 4.4.7 Influence of gamma rays treatments on number of sprouts forming plantlet | 第63-64页 |
| 4.4.8 Influence of gamma rays treatments on plantlet length | 第64页 |
| 4.4.9 Influence of gamma rays treatments on root length | 第64-65页 |
| 4.4.10 Influence of gamma rays treatments on root number | 第65页 |
| 4.4.11 Influence of gamma rays treatments on leaves number per plantlet | 第65-66页 |
| 4.4.12 Influence of gamma rays treatments on bulblet diameter | 第66-67页 |
| 4.4.13 Identification of the mutants using SSR marker | 第67-68页 |
| 4.4.14 Morphological identification of variations | 第68页 |
| 4.5 Discussion | 第68-71页 |
| Chapter Ⅴ:Influence of Ethyl Methane Sulphonate on performance of garlic callus,regenerated sprouts and formed plantlets | 第71-82页 |
| 5.1 Abstract | 第71页 |
| 5.2 Introduction | 第71-72页 |
| 5.3 Materials and methods | 第72-73页 |
| 5.3.1 Ethyl methane sulphonate (EMS) treatment | 第72页 |
| 5.3.2 Shoot, root induction from callus and plantlet acclimatization | 第72-73页 |
| 5.3.4 Observation and statistical analysis | 第73页 |
| 5.4 Results | 第73-80页 |
| 5.4.1 Influence of EMS treatments on average number of sprouts per callus | 第73-74页 |
| 5.4.2 Influence of EMS treatments on total number of sprouts | 第74-75页 |
| 5.4.3 Influence of EMS treatments on number of sprouts forming plantlet | 第75-76页 |
| 5.4.4 Influence of EMS treatment on plantlet length | 第76-77页 |
| 5.4.5 Influence of EMS treatments on leaves number per plantlet | 第77页 |
| 5.4.6 Influence of EMS treatments on root number | 第77-78页 |
| 5.4.7 Influence of EMS treatments on root length | 第78-79页 |
| 5.4.8 Influence of EMS treatments on bulblet diameter | 第79页 |
| 5.4.9 Morphological variance of the plantlets from callus treated with EMS | 第79-80页 |
| 5.5 Discussion | 第80-82页 |
| Conclusion | 第82-84页 |
| References | 第84-94页 |
| Acknowledgements | 第94-96页 |
| Resume | 第96-97页 |
