| ABSTRACT | 第3-4页 |
| 摘要 | 第5-8页 |
| CHAPTER 1: INTRODUCTION AND LITERATURE REVIEW | 第8-20页 |
| 1.1. Brief background | 第8-9页 |
| 1.2. Applications of glutamate | 第9-11页 |
| 1.2.1. Food industry | 第9-10页 |
| 1.2.2. Medical/Pharmaceutical industries | 第10页 |
| 1.2.3. Research Products, Analytical Standards and Chemical Intermediate | 第10-11页 |
| 1.2.4. Other applications | 第11页 |
| 1.3. Industrial fermentation of glutamate | 第11-14页 |
| 1.4. Literature review | 第14-20页 |
| 1.4.1. The microorganism: Corynebacterium glutamicum | 第14页 |
| 1.4.2. Central metabolism of Corynebacterium glutamicum in brief | 第14-16页 |
| 1.4.3. The problem | 第16-20页 |
| CHAPTER 2 MATERIALS AND METHODS | 第20-24页 |
| 2.1. Strain | 第20页 |
| 2.2. Shake flasks seed and fermentation media composition and conditions | 第20页 |
| 2.3. Fermentation media composition and conditions in 5 L bioreactor | 第20-21页 |
| 2.3.1. Fermentation conditions | 第20-21页 |
| 2.3.2. Fermentation medium | 第21页 |
| 2.4. Analytical methods | 第21-24页 |
| 2.4.1. Concentrations of cell, glucose and glutamate | 第21-22页 |
| 2.4.2. Determination of CO_2release amount and yields of glutamate/CO_2on glucose | 第22页 |
| 2.4.3. Enzyme activity assays | 第22-24页 |
| CHAPTER 3. RESULTS AND DISCUSSION | 第24-44页 |
| 3.1. Preliminary experiments in shake flasks | 第24-26页 |
| 3.2. Fermentation performance and enzymatic activities changes when pH and NaHCO_3 addition were singly regulated | 第26-30页 |
| 3.2.1. NaHCO_3addition only | 第26-28页 |
| 3.2.2. pH increase only | 第28页 |
| 3.2.3. Addition of both NaHCO_3and HEPE | 第28-30页 |
| 3.3. Fermentation performance and enzymatic activities changes when pH and NaHCO_3addition were jointly regulated | 第30-36页 |
| 3.3.1. Simultaneous increase of pH and commencement of NaHCO_3addition | 第30-34页 |
| 3.3.2. Increase of pH first and commencement of NaHCO_3addition later | 第34-35页 |
| 3.3.3. Commencement of NaHCO_3addition first and increase of pH later | 第35-36页 |
| 3.4. Metabolic flux analysis of glutamate fermentations under different pH/NaHCO_3regulation modes | 第36-44页 |
| 3.4.1 Calculation principle or methods of metabolic flux analysis | 第37-39页 |
| 3.4.2 The results of metabolic flux analysis | 第39-44页 |
| GENERAL CONCLUSION | 第44-46页 |
| RECOMMENDATIONS FOR FURTHER STUDY | 第46-48页 |
| ACKNOWLEDGEMENTS | 第48-50页 |
| REFERENCES | 第50-56页 |
| List of publications | 第56页 |
