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The Effects of Selenium-enriched Probiotics on Growth Performances,Oocysts Shedding and Intestinal Lesions of Local Chinese Chickens Infected with Eimeria Tenella

ACRONYMS第11-12页
ABSTRACT第12页
摘要第13-14页
CHAPTER 1 INTRODUCTION第14-18页
    1.1. Background of the study第14页
    1.2. Statement of the problem第14-16页
    1.3. Objectives of the study第16-18页
CHAPTER 2 LITERATURE REVIEW第18-36页
    2.1. Coccidiosis第18-22页
        2.1.1. Life cycle第18-20页
        2.1.2. Ethiology第20-21页
        2.1.3. Site of specificity第21页
        2.1.4. Immune system第21-22页
    2.2. Probiotic第22-27页
        2.2.1. Mechanism of action of probiotic第24-25页
        2.2.2. Lactobacillus acidophilus第25-26页
        2.2.3. Saccharomyces cerevisiae第26-27页
        2.2.4. Nutritional and therapeutic purposes of probiotics第27页
    2.3. Selenium第27-33页
        2.3.1. Biochemistry of selenium第28-29页
        2.3.2. Selenoproteins第29-30页
        2.3.3. Metabolism第30页
        2.3.4. Absorption第30-31页
        2.3.5. Transport and storage第31页
        2.3.6. Bioavailability第31-32页
        2.3.7. Deficiency第32页
        2.3.8. Excretion第32-33页
        2.3.9. Selenium as source of antioxidant and immunity system第33页
    2.4. Selenium-enriched probiotics第33-36页
        2.4.1. Antioxidative activity第34页
        2.4.2. Improved the nutritional value and lowered toxicity第34-35页
        2.4.3. Fermented food and health benefits of selenium-enriched probiotics第35-36页
CHAPTER 3 MATERIALS AND METHODS第36-44页
    3.1. Sterilization of materials第36页
    3.2. Probiotics第36页
    3.3. Preparation of probiotics media第36-37页
        3.3.1. De Man, Rogosa and Sharpe media (MRS)第36-37页
        3.3.2. Yeast Extract Peptone Dextrose media (YEPD)第37页
        3.3.3. Activation of probiotic strains第37页
        3.3.4. Preparation of broth第37页
        3.3.5. Preparation of SP (conversion of inorganic Se into organic Se)第37页
    3.4. Selenium-enriched probiotics, sodium selenite and probiotics第37-38页
    3.5. Animals and experimental design第38-39页
    3.6. Oocyst preparation and chickeninfection第39-40页
    3.7. Sample collection第40-41页
        3.7.1. Collection of oocysts第40页
        3.7.2. Blood sample第40页
        3.7.3. Histopathological examination第40-41页
    3.8. Biochemical analysis of blood parameters第41页
    3.9. RNA extraction and determination of mRNA gene expression第41-42页
    3.10. Statistical analysis第42-44页
CHAPTER 4 RESULTS第44-54页
    4.1. Growth performances第44-46页
    4.2. Oocysts shedding第46-47页
    4.3. Caecal lesion score第47-48页
    4.4. Histopathological examination第48-49页
    4.5. Biochemical analysis第49-50页
    4.6. Antioxidant capacities第50-52页
        4.6.1. Malondialdehyde (MDA) serum contents第50页
        4.6.2. Superoxide dismutase (SOD) serum activity第50-51页
        4.6.3. Glutathione peroxidase-1 (GPx1)activity第51-52页
        4.6.4. Catalase (CAT) activity第52页
    4.7. Gene mRNA expression第52-54页
CHAPTER 5 DISCUSSION第54-62页
    5.1. Growth performances第54-55页
    5.2. Oocysts shedding and intestinal lesions score第55-56页
    5.3. Serum biochemical analysis第56-57页
    5.4. Antioxidant capacities第57-58页
    5.5. mRNA gene expressions第58-62页
CHAPTER 6 CONCLUSIONS AND RECOMMENDATIONS第62-64页
    6.1. Conclusions第62页
    6.2. Recommendations第62-64页
CHAPTER 7 References第64-76页
SUMMARY第76-78页
INNOVATION第78-80页
LIST OF PUBLICATION第80-82页
DEDICATION第82-84页
ACKNOWLEDGEMENTS第84页

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