| 致谢 | 第10-12页 |
| Abstract | 第12-15页 |
| 摘要 | 第16-19页 |
| 缩略词 | 第19-22页 |
| Chapter Ⅰ:Literature Review | 第22-63页 |
| 1 Interaction between Salmonella Infection and Host Defense | 第22-33页 |
| 1.1 Overview of Salmonella | 第22页 |
| 1.2 Virulence Mechanisms | 第22-24页 |
| 1.3 Salmonella Infection | 第24-26页 |
| 1.3.1 Local Mucosal Invasion of Salmonella | 第24-26页 |
| 1.3.2 Systemic Dissemination of Salmonella | 第26页 |
| 1.4 Host Defense | 第26-28页 |
| 1.4.1 Detection of Salmonella by PRRs | 第26-27页 |
| 1.4.2 Ampliation of The Inflammatory Response | 第27-28页 |
| 1.4.3 Phagocytes Infiltration | 第28页 |
| 1.5 Conclusion | 第28-29页 |
| References | 第29-33页 |
| 2 The Role of NADPH Oxidase in Host Defense | 第33-48页 |
| 2.1 Overview of NADPH Oxidase | 第33页 |
| 2.2 The NOX Family | 第33-38页 |
| 2.2.1 Phagocyte NADPH Oxidase | 第34-35页 |
| 2.2.2 NOX2 Homologues | 第35-36页 |
| 2.2.3 Regulatory Subunits | 第36-38页 |
| 2.3 NADPH Oxidase-derived ROS | 第38-39页 |
| 2.4 Host Defense and Inflammation | 第39-42页 |
| 2.4.1 Microbicidal Activity | 第39-40页 |
| 2.4.2 Immune Modulatory Effects | 第40-42页 |
| References | 第42-48页 |
| 3 The Role of Autophagy in Salmonella Infection | 第48-62页 |
| 3.1 Overview of Autophagy | 第48-50页 |
| 3.2 Interaction between Salmonella and Antibacterial Autophagy | 第50-56页 |
| 3.2.1 Salmonella Is Targeted for Antibacterial Autophagy | 第50-55页 |
| 3.2.2 Salmonella Manipulates Autophagy | 第55-56页 |
| 3.3 Conclusion | 第56-57页 |
| References | 第57-62页 |
| 4 Aims and Objectives | 第62-63页 |
| Chapter Ⅱ:Methology and Data Analysis | 第63-151页 |
| 1 The Role of p40~(phox) in Host Defense against Systemic Salmonella Infection | 第63-93页 |
| 1.1 Introduction | 第63-64页 |
| 1.2 Materials and Methods | 第64-74页 |
| 1.2.1 Mice | 第64页 |
| 1.2.2 Reagents | 第64-65页 |
| 1.2.3 Laboratory Equipment | 第65-66页 |
| 1.2.4 Methods | 第66-74页 |
| 1.3 Data Analysis and Findings | 第74-90页 |
| 1.3.1 p40~(phox) deficiency increases mortality and bodyweight changes of mice during Salmonella infection | 第74-75页 |
| 1.3.2 p40~(phox) deficiency exacerbates bacterial translocation in Salmonella-infected mice | 第75-76页 |
| 1.3.3 p40~(phox-/-) mice develop more severe spleen and liver inflammation after oral S. Typhimurium infection | 第76-79页 |
| 1.3.4 p40~(phox) deficiency in S. Typhimurium-infected mice enhances the recruitment of macrophages and neutrophils after oral infection | 第79-82页 |
| 1.3.5 p40~(phox) deficiency in S. Typhimurium-infected mice upregulates the expression of chemoattractants in spleens after oral infection | 第82-83页 |
| 1.3.6 p40~(phox) deficiency in S. Typhimurium-infected mice increases the CD4~+ T cells recruitment after oral infection | 第83-84页 |
| 1.3.7 p40~(phox) deficient mice exhibit normal ability to recruit phagocytes to the peritoneal cavity after intra-peritoneal infection with Salmonella | 第84-86页 |
| 1.3.8 p40~(phox) deficiency exacerbates tissue inflammation and bacterial translocation in Salmonella-infected mice after intra-peritoneal infection | 第86-88页 |
| 1.3.9 p40~(phox) deficiency in S. Typhimurium-infected mice enhances the recruitment of macrophages and neutrophils to spleen after intra-peritoneal infection | 第88-90页 |
| 1.4 Discussion | 第90-91页 |
| References | 第91-93页 |
| 2 The Role of p40~(phox) in Regulating Intestinal Inflammation Caused by SalmonellaInfection | 第93-112页 |
| 2.1 Introduction | 第93页 |
| 2.2 Materials and Methods | 第93-97页 |
| 2.2.1 Mice | 第93-94页 |
| 2.2.2 Reagents | 第94页 |
| 2.2.3 Laboratory Equipment | 第94页 |
| 2.2.4 Methods | 第94-97页 |
| 2.3 Data Analysis and Findings | 第97-108页 |
| 2.3.1 p40~(phox) deficiency exacerbates Salmonella-induced mucosal injury | 第97-98页 |
| 2.3.2 p40~(phox) deficiency alters the distribution of specific junctional proteins in cecal epithelium in Salmonella-infected mice | 第98-100页 |
| 2.3.3 p40~(phox) deficiency exacerbates bacterial translocation in Salmonella-infected mice | 第100-101页 |
| 2.3.4 p40~(phox) deficiency in 5. Typhimurium-infected mice regulates the expression of mucins and enzymes involved in glycan modifications | 第101-104页 |
| 2.3.5 Pretreatment with streptomycin induces intense intestinal inflammation in Salmonella-infected mice | 第104-105页 |
| 2.3.6 p40~(phox) deficiency in S. Typhimurium-infected mice enhances the recruitment of macrophages and neutrophils in intestinal mucosa | 第105-107页 |
| 2.3.7 p40~(phox) deficiency in S. Typhimurium-infected mice alters the distribution of macrophages and neutrophils in intestinal mucosa | 第107-108页 |
| 2.4 Discussion | 第108-110页 |
| References | 第110-112页 |
| 3 The Role of p40~(phox) in Regulating Antimicrobial Activity of Macrophages | 第112-129页 |
| 3.1 Introduction | 第112-113页 |
| 3.2 Materials and Methods | 第113-117页 |
| 3.2.1 Mice | 第113页 |
| 3.2.2 Reagents | 第113-114页 |
| 3.2.3 Laboratory Equipment | 第114页 |
| 3.2.4 Methods | 第114-117页 |
| 3.3 Data Analysis and Findings | 第117-125页 |
| 3.3.1 p40~(phox-/-) macrophages exhibit normal bacterial phagocytosis and reduced bactericidal activity | 第117-119页 |
| 3.3.2 p40~(phox-/-) macrophages and p40~(phox-/-) neutrophils exhibit severe defects in PMA-induced ROS production | 第119-120页 |
| 3.3.3 p40~(phox-/-) macrophages have compromised autophagic killing of Salmonella | 第120-123页 |
| 3.3.4 p40~(phox-/-) macrophages produce an elevated amount of IL-1β induced by Samonella antigen stimulation and exhibit enhanced inflammasome activation | 第123-125页 |
| 3.4 Discussion | 第125-127页 |
| References | 第127-129页 |
| 4 The Role of p40~(phox) in Host Defense against Citrobacter rodentium Infection | 第129-151页 |
| 4.1 Introduction | 第129-130页 |
| 4.2 Materials and Methods | 第130-134页 |
| 4.2.1 Mice | 第130页 |
| 4.2.2 Reagents | 第130-131页 |
| 4.2.3 Laboratory Equipment | 第131页 |
| 4.2.4 Methods | 第131-134页 |
| 4.3 Data Analysis and Findings | 第134-143页 |
| 4.3.1 p40~(phox) deficiency does not increase susceptibility of mice to C. rodentium infection | 第134-135页 |
| 4.3.2 p40~(phox) deficiency does not exacerbate C. rodentium-induced colonic pathology | 第135-137页 |
| 4.3.3 Differential cytokine expression in C. rodentium-infected p40~(phox) -deficient mice | 第137-139页 |
| 4.3.4 p40~(phox) deficiency alters inflammatory cell infiltration of the colonic mucosa | 第139-140页 |
| 4.3.5 Altered cytokine production in p40~(phox) -deficient mice with C. rodentium infection | 第140-142页 |
| 4.3.6 p40~(phox) deficiency increases iNOS expression and NO production in response to C. rodentium infection | 第142-143页 |
| 4.4 Discussion | 第143-147页 |
| References | 第147-151页 |
| Chapter Ⅲ:Conclusion and Future Work | 第151-152页 |
| 1 Concluding Statements | 第151页 |
| 2 Suggested Future Work | 第151-152页 |
| 个人简历 | 第152-153页 |
