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基于SNP-CAPS标记的甜瓜遗传连锁图谱构建及果实相关性状QTL分析

摘要第10-12页
Abstract第12-13页
1 Introduction第14-58页
    1.1 Fruits第14-15页
    1.2 Melon introduction and its classification第15-16页
    1.3 Melon botany and its origin第16-17页
    1.4 Physiological changes occur during ripening in melon第17-19页
    1.5 Biochemical changes occur during ripening in melon第19-24页
        1.5.1 Carbohydrate metabolism第19-21页
        1.5.2 Organic acids第21-22页
        1.5.3 Cell wall metabolism第22-24页
    1.6 Molecular markers第24-32页
        1.6.1 Categories of molecular markers第25页
        1.6.2 Dominant DNA markers第25-26页
        1.6.3 Co-dominant markers第26-32页
    1.7 Sequencing technology第32-35页
        1.7.1 High-throughput sequencing第32页
        1.7.2 Application of High-throughput sequencing第32-35页
    1.8 Quantitative trait loci (QTL)第35-40页
        1.8.1 Mapping population第37-39页
        1.8.2 Linkage map analysis第39页
        1.8.3 Determination of genetic distance第39-40页
        1.8.4 Statistical methods to detect QTL第40页
    1.9 Various genetic basis studies for QTL detection in melon and other fruits第40-56页
    1.10 Objectives of current research第56-57页
    1.11 Technical route of research第57页
    1.12 Annual research plan第57-58页
2 Materials and methods第58-70页
    2.1 Field trials and methods第58-61页
        2.1.1 Experimental material第58页
        2.1.2 Greenhouse test design第58-59页
        2.1.3 Seed treatment第59页
        2.1.4 Raising of nursery seedlings第59-60页
        2.1.5 Selfing of F2:3 plant population第60页
        2.1.6 Phenotypic evaluation for fruit traits第60-61页
    2.2 Molecular experiment reagents and instruments第61-65页
        2.2.1 Required reagents第61-62页
        2.2.2 Molecular experiment instruments第62页
        2.2.3 Preparation of required reagents第62-65页
    2.3 Molecular experiment procedures第65-68页
        2.3.1 Sequencing library assembling第65-66页
        2.3.2 Assembling of CAPS markers by High-throughput sequencing information第66页
        2.3.3 CAPS markers designing第66-67页
        2.3.4 PCR for CAPS markers verification and genotyping第67-68页
        2.3.5 Restriction endonuclease (RE) digestion reaction第68页
    2.4 Molecular markers data analysis method第68-70页
        2.4.1 CAPS markers genotyping data analysis procedure and method第68页
        2.4.2 Genetic map assembling and QTL analysis第68-69页
        2.4.3 Statistical analysis第69-70页
3 Results and analysis第70-86页
    3.1 Analysis of SNPs distribution on chromosomes of melon genome第70-71页
    3.2 Analysis of CAPS polymorphism screening and DNA detection第71-72页
    3.3 Analysis of linkage map construction through novel CAPSs第72-74页
    3.4 Analysis of melon fruit traits第74-79页
        3.4.1 Analysis of fruit weight第74页
        3.4.2 Analysis of fruit firmness第74-75页
        3.4.3 Analysis of fruit length and width第75页
        3.4.4 Analysis of fruit shape第75页
        3.4.5 Analysis of flesh firmness第75页
        3.4.6 Analysis of Brix第75-76页
        3.4.7 Analysis of p H第76页
        3.4.8 Analysis of TA (titratable acidity)第76-79页
    3.5 Anaysis of QTLs related to fruit traits第79-86页
        3.5.1 Analysis of fruit weight QTLs第79页
        3.5.2 Analysis of fruit firmness QTLs第79-80页
        3.5.3 Analysis of flesh firmness QTLs第80页
        3.5.4 Analysis of Brix QTLs第80-81页
        3.5.5 Analysis of fruit length, width and shape QTLs第81-82页
        3.5.6 Analysis of p H QTLs第82页
        3.5.7 Analysis of TA (titratable acidity) QTLs第82-86页
4 Discussion第86-94页
    4.1 Importance of molecular markers第86页
    4.2 Linkage map assembling by CAPS markers第86-88页
    4.3 Detection of melon fruit traits QTLs第88-92页
        4.3.1 Fruit weight QTLs第88-89页
        4.3.2 Fruit length, width and shape QTLs第89-90页
        4.3.3 Fruit firmness and flesh firmness QTLs第90-91页
        4.3.4 Fruit Brix QTLs第91-92页
        4.3.5 Fruit pH and TA QTLs第92页
    4.4 Innovative points第92-94页
5 Conclusion第94-95页
    5.1 Fruit traits segregation第94页
    5.2 SNP-CAPS markers assembling第94页
    5.3 Linkage mapping and QTL analysis第94-95页
Acknowledgement第95-96页
References第96-115页
Appendix第115-119页
Papers published during the time period of Ph.D education第119页

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