| 摘要 | 第5-7页 |
| Abstract | 第7-9页 |
| Part Ⅰ Introduction | 第10-34页 |
| 1. Introduction of electrochemical biosensor | 第10-12页 |
| 1.1 The properties of deoxyribonucleic acid | 第10-11页 |
| 1.2 The theory of electrochemical biosensor | 第11页 |
| 1.3 The classification of electrochemical biosensor | 第11-12页 |
| 2. The study of DNA-based electrochemical sensor for analysis and testing | 第12-22页 |
| 2.1 The assay of DNA-based electrochemical sensor for detection of DNA sequence | 第12-18页 |
| 2.1.1 Electrochemical-active indicator labeled DNA-based electrochemical sensors | 第12-15页 |
| 2.1.2 Gold nanoparticles labeled DNA-based electrochemical sensors | 第15-16页 |
| 2.1.3 Quantum dots labeled DNA-based electrochemical sensors | 第16页 |
| 2.1.4 Enzymes labeled DNA-based electrochemical sensors | 第16-17页 |
| 2.1.5 Label-free DNA-based electrochemical sensors | 第17-18页 |
| 2.2 The assay of DNA-based electrochemical sensor for detection of heavy metal ions | 第18-21页 |
| 2.2.1 DNA-based electrochemical sensors for detection of Ag~+ and Hg~(2+) based on metal-mediated base pairs | 第19页 |
| 2.2.2 DNA-based electrochemical sensors for detection of Pb~(2+) | 第19-21页 |
| 2.3 Other assays of DNA-based electrochemical sensors | 第21-22页 |
| 2.3.1 DNA-based electrochemical sensing for the assay of DNA methyltransferase and inhibitor screening | 第21页 |
| 2.3.2 DNA-based electrochemical sensing for detection of pH | 第21-22页 |
| 3. The significance of this study | 第22-23页 |
| References | 第23-34页 |
| Part Ⅱ Main research works | 第34-78页 |
| 1. A sensitive and label-free impedimetric biosensor based on an adjunct probe | 第34-52页 |
| 1.1 Introduction | 第34-36页 |
| 1.2 Experimental | 第36-38页 |
| 1.2.1 Chemicals and reagents | 第36-37页 |
| 1.2.2 Apparatus | 第37页 |
| 1.2.3 Fabrication of the biosensor | 第37-38页 |
| 1.3 Results and discussion | 第38-46页 |
| 1.3.1 Design strategy of adjunct probe-aided electrochemical impedance biosensor | 第38-39页 |
| 1.3.2 Effect of adjunct probe concentration | 第39-40页 |
| 1.3.3 Optimization of hybridization time | 第40页 |
| 1.3.4 Characterization of the electrochemical impedance biosensor | 第40-42页 |
| 1.3.5 Improved sensitivity by the adjunct probe | 第42-43页 |
| 1.3.6 Detection of target DNA with electrochemical impedance biosensor | 第43-44页 |
| 1.3.7 Selectivity of the biosensor | 第44-45页 |
| 1.3.8 Analytical application of the biosensor | 第45-46页 |
| 1.4 Conclusions | 第46-47页 |
| References | 第47-52页 |
| 2. Crystal violet as an i-motif structure probe for reversible and label-free electrochemical pH sensor | 第52-66页 |
| 2.1 Introduction | 第52-54页 |
| 2.2 Experimental section | 第54-55页 |
| 2.2.1 Reagents | 第54页 |
| 2.2.2 Apparatus | 第54-55页 |
| 2.2.3 Fabrication and preparation of the electrochemical pH sensor | 第55页 |
| 2.2.4 Electrochemical measurements of pH sensor | 第55页 |
| 2.3 Results and discussion | 第55-62页 |
| 2.3.1 Design strategy of the electrochemical pH sensor | 第55-57页 |
| 2.3.2 Opitmizaiton of condiitons | 第57-59页 |
| 2.3.3 Differential pulse voltammetry tests of pH sensor in different pH buffers | 第59-61页 |
| 2.3.4 Reversibility and selectivity of pH sensor | 第61-62页 |
| 2.4 Conclusions | 第62-63页 |
| References | 第63-66页 |
| 3. A simple electrochemical biosensor for detection of silver(Ⅰ) ions and cysteine based on the label-free cytosine-rich ssDNA | 第66-78页 |
| 3.1 Introduction | 第66-67页 |
| 3.2 Experimental section | 第67-69页 |
| 3.2.1 Reagents | 第67-68页 |
| 3.2.2 Apparatus | 第68页 |
| 3.2.3 Fabrication of the biosensor | 第68-69页 |
| 3.2.4 Ag~+ detection | 第69页 |
| 3.2.5 Cys detection | 第69页 |
| 3.3 Results and discussion | 第69-74页 |
| 3.3.1 Design strategy of the electrochemical biosensor | 第69-70页 |
| 3.3.2 Characterization of the electrochemical impedance biosensor | 第70-72页 |
| 3.3.3 Sensitivity and selectivity of biosensor for detection of Ag~+ | 第72-73页 |
| 3.3.4 Sensitivity and selectivity of biosensor for detection of Cys | 第73-74页 |
| 3.4 Conclusions | 第74-75页 |
| References | 第75-78页 |
| Part Ⅲ Conclusions and outlook | 第78-80页 |
| Appendix:Paper published during the graduate | 第80-82页 |
| 致谢 | 第82页 |
