| Abstract | 第7-8页 |
| List of abbreviations | 第9-11页 |
| 1 Introduction | 第11-17页 |
| 2 Review of Literature | 第17-38页 |
| 2.1 Importance and global production of citrus | 第17-18页 |
| 2.2 Nutritional Importance of Citrus | 第18-20页 |
| 2.3 Genomics and its role in plant improvement | 第20-21页 |
| 2.4 Bacterial Artificial Chromosome Library | 第21-22页 |
| 2.5 Development of BAC vector(pCUGIBAC1) | 第22-24页 |
| 2.6 BAC library applications in the development of plant genomics | 第24-33页 |
| 2.6.1 Use of BAC clones for molecular marker development | 第25-28页 |
| 2.6.2 Map-based/Positional cloning | 第28-29页 |
| 2.6.3 Physical Mapping | 第29-32页 |
| 2.6.4 BAC library applications in citrus genomics | 第32-33页 |
| 2.7 Flourescence In situ hybridization(FISH) | 第33-35页 |
| 2.7.1 BAC-FISH applications in plant genetics | 第34-35页 |
| 2.8 Genomic in situ hybridization(GISH) | 第35-38页 |
| 3 Materials and Methods | 第38-46页 |
| 3.1 Plant material | 第38页 |
| 3.2 BAC vector preparation | 第38-39页 |
| 3.3 Preparation of high-molecular-weight(HMW) DNA | 第39-40页 |
| 3.4 Determination of Optimal Partial Digestion Conditions | 第40页 |
| 3.5 Ligation and Transformation | 第40-41页 |
| 3.6 Preparation of BAC DNA and estimation of insert size | 第41-42页 |
| 3.7 Screening by Southern hybridization | 第42-43页 |
| 3.8 PCR-based screening | 第43-44页 |
| 3.9 Slide preparation | 第44页 |
| 3.10 DNA extraction,probe labelling and GISH(Genomic In Situ Hybridization) analyses | 第44-46页 |
| 4 Results | 第46-65页 |
| 4.1 Preparation of BAC vector | 第46-49页 |
| 4.1.1 λ DNA transformation for efficiency check | 第48-49页 |
| 4.2 Preparation of High Molecular Weight(HMW) inserts DNA | 第49-53页 |
| 4.2.1 Partial digestion of HMW genomic DNA | 第50-52页 |
| 4.2.2 First size and second size selection | 第52-53页 |
| 4.3 Construction and insert sizing of citrus BAC library | 第53-55页 |
| 4.3.1 Statistics of the library | 第54-55页 |
| 4.4 Characterization of the C.sinensis BAC library | 第55-59页 |
| 4.4.1 Isolation of BAC clones containing phytoene synthase(Psy) gene | 第58页 |
| 4.4.2 PCR Based screening | 第58-59页 |
| 4.5 Insert size analysis of positive clones | 第59-60页 |
| 4.6 DAPI staining of C.sinensis chromosomes | 第60-62页 |
| 4.7 Genomic In situ Hybridization analysis(GISH) | 第62-65页 |
| 5 Discussions | 第65-76页 |
| 5.1 BAC library and its application in citrus | 第65-67页 |
| 5.2 Quality Assessments of the BAC library of C.sinensis | 第67-69页 |
| 5.3 Important steps in the construction of the library | 第69-72页 |
| 5.4 DAPI and GISH analysis in citrus | 第72-74页 |
| 5.5 Conclusion and Future prospects | 第74-76页 |
| REFERENCES | 第76-107页 |
| Protocols | 第107-128页 |
| Acknowledgement | 第128-131页 |
| CURRICULUM VITAE | 第131-132页 |
| ACDEMIC EXPERIENCE | 第132-137页 |
