ABSTRACT | 第1-16页 |
中文摘要 | 第16-19页 |
CHAPTER ONE GENERAL INTRODUCTION AND LITERATURE REVIEW | 第19-56页 |
·General introduction | 第19-21页 |
·Stored products and their environment | 第21-23页 |
·Postharvest food losses and causes | 第23-24页 |
·The Indianmeal moth | 第24-27页 |
·Trends in food storage and pest control | 第27-42页 |
·Cultural methods | 第28-29页 |
·Hygienic and sanitary measures | 第29页 |
·Structural methods | 第29页 |
·Grain protectants | 第29-31页 |
·Chemical control | 第31-38页 |
·Biological control in stored products | 第38-42页 |
·Bacillus thuringiensis | 第42-49页 |
·Application of Bacillus thuringiensis pesticides | 第42-44页 |
·Revised Bacillus thuringiensis insecticidal crystal protein | 第44-49页 |
·Habrobracon hebetor Say | 第49-50页 |
·Combination of two or more biological control techniques | 第50-53页 |
·Dissertation objectives | 第53-56页 |
CHAPTER TWO GENERAL MATERIALS AND METHODS | 第56-59页 |
·Bacillus thuringiensis strains | 第56-57页 |
·Collection and rearing of test insects | 第57-58页 |
·Preparation of artificial diet | 第58-59页 |
CHAPTER THREE PARASITISM AND HOST-LOCATION PREFERENCE IN HABROBRACON HEBETOR(HYMENOPTERA:BRACONIDAE):ROLE OF REFUGE,CHOICE AND HOST-INSTAR | 第59-75页 |
·Introduction | 第59-60页 |
·Material and methods | 第60-64页 |
·Collection and rearing of test insects | 第60-61页 |
·Experimental procedures | 第61-63页 |
·Data analysis | 第63-64页 |
·Results | 第64-71页 |
·Effect factors on parasitism by Habrobracon hebetor | 第64-69页 |
·Effects of Host-instars on Habrobracon hebetor Progeny | 第69-71页 |
·Discussion | 第71-75页 |
CHAPTER FOUR RESPONSE OF PLODIA INTERPUNCTELLA(LEPIDOPTERA:PYRALIDAE)TO TREATMENTS WITH DIFFERENT STRAINS OF BACILLUS THURINGIENSIS | 第75-93页 |
·Introduction | 第75-76页 |
·Material and methods | 第76-82页 |
·Insect culture | 第76-77页 |
·Bacillus thuringiensis strains | 第77页 |
·Bioassays | 第77-80页 |
·Serological tests | 第80页 |
·DNA extraction,PCR and SDS-PAGE | 第80-81页 |
·Statistical analysis | 第81-82页 |
·Results | 第82-90页 |
·Insect bioassay and toxicity | 第82-86页 |
·Characterization of isolates with high toxicity to Indianmeal moth | 第86-90页 |
·Discussion | 第90-93页 |
CHAPTER FIVE EFFECT OF BACILLUS THURINGIENSIS ON HABROBRACON HEBETOR DURING COMBINE BIOLOGICAL CONTROL OF PLODIA INTERPUNCTELLA | 第93-113页 |
·Introduction | 第93-96页 |
·Materials and methods | 第96-101页 |
·Collection and rearing of test insects | 第96页 |
·Bacillus thuringiensis strains and media | 第96-97页 |
·Purification of Bacillus thuringiensis spores and crystals | 第97页 |
·Preliminary assay of 50%lethal concentrations(LC_(50)) to Plodia interpunctella | 第97-98页 |
·Host(Plodia interpunctella) location and acceptance by Habrobracon hebetor | 第98-99页 |
·Effect of combining Bacillus thuringiensis with Habrobracon hebetor for control of Plodia interpunctella | 第99-101页 |
·Statistical analysis | 第101页 |
·Results | 第101-109页 |
·Preliminary assay of 50%lethal concentrations(LC_(50)) to Plodia interpunctella | 第101-102页 |
·Host(Plodia interpunctella) location and acceptance by Habrobracon hebetor | 第102-104页 |
·Effect of varying treatments on Plodia interpunctella mortality | 第104-105页 |
·Effect of Bacillus thuringiensis-intoxicated hosts on Habrobracon hebetor progeny development | 第105-109页 |
·Discussion | 第109-113页 |
·Mortality effect of treatments on Plodia interpunctella | 第109-110页 |
·Influence of Bacillus thuringiensis-intoxicated hosts on Habrobracon hebetor development | 第110-113页 |
CHAPTER SIX KAIROMONE EXTRACTED FROM FRASS AND LARVA OF INDIANMEAL MOTH,ELICITS OVIPOSITION BEHAVIOUR IN HABROBRACON HEBETOR | 第113-143页 |
·Introduction | 第113-115页 |
·Methods and materials | 第115-124页 |
·Collection and rearing of insects | 第115页 |
·Extraction | 第115-116页 |
·Isolation and identification | 第116-117页 |
·Thin-layer chromatography | 第117页 |
·Gas chromatography | 第117页 |
·Gas Chromatography-Mass Spectrometry | 第117-118页 |
·Testing effects on parasitization rates on P.interpunctella-related materials | 第118-120页 |
·Responses of the parasitoid to extracts of materials from P.interpunctella | 第120-121页 |
·Behavioral responses of female parasitoids to isolates from P.interpunctella-related materials | 第121-122页 |
·Statistical analyses | 第122-124页 |
·Results | 第124-139页 |
·Response of Habrobracon hebetor females to Plodia interpunctella-related materials | 第124-127页 |
·Response of Habrobracon hebetor females to extracts from Plodia interpunctella-related materials | 第127-130页 |
·Response of Habrobracon hebetor females to fractions isolated from frass or larva of Plodia interpunctella | 第130-137页 |
·Identification of hexane soluble active fraction isolated from frass and fifth instar larva of Plodia interpunctella | 第137-139页 |
·Discussion | 第139-143页 |
·Response to Plodia interpunctella related materials by Habrobracon hebetor | 第139-140页 |
·Effect of extraction solvents and the response of Habrobracon hebetor to extracts from Plodia interpunctella-related materials | 第140-141页 |
·Active compounds identification from fraction isolated from frass and fifth instar larva of Plodia interpunctella | 第141-143页 |
GENERAL CONCLUSIONS AND RECOMMENDATIONS | 第143-146页 |
LITERATURES CITED | 第146-178页 |
ACKNOWLEDGEMENTS | 第178-180页 |
ARTICLES PUBLISHED DURING THE PHD PROGRAM | 第180-181页 |