| Abstract | 第5-8页 |
| 摘要 | 第9-18页 |
| Chapter ⅠIntroduction | 第18-29页 |
| 1.1. Overview | 第18-19页 |
| 1.2. Genetic variation in viral genome and their geographical distribution | 第19-20页 |
| 1.3. Progress of research for in diagnosis | 第20-21页 |
| 1.4. The purpose of this study | 第21-22页 |
| 1.5. The significance of this study | 第22页 |
| REFERENCES | 第22-29页 |
| Chapter No Ⅱ Development of Magnetic Beads Based Protocol for the Purification of High Quality NucleicAcids from Cells,Bacteria and Virus | 第29-53页 |
| 2.1 Introduction | 第29-32页 |
| 2.2 Materials and Methods | 第32-36页 |
| 2.2.1 Materials | 第32-33页 |
| 2.2.2 Methods | 第33-36页 |
| 2.3 Results and Discussion | 第36-49页 |
| 2.3.1 Viral Genome Extraction | 第36-43页 |
| 2.3.2 Nucleic Acid Extraction from Cells(Hep G2 Cells) | 第43-46页 |
| 2.3.3 Nucleic acid Extraction from Bacteria(E.coli strain DH5α) | 第46-49页 |
| 2.4 Conclusion | 第49-50页 |
| 2.4.1 Preparation of NA extraction kit | 第49页 |
| 2.4.2 Application of NA extraction kit for viral genome extraction | 第49页 |
| 2.4.3 Application of NA extraction kit for NA extraction from cell culture | 第49页 |
| 2.4.4 Application of NA extraction kit for NA extraction from Bacteria | 第49-50页 |
| REFERENCES | 第50-53页 |
| Chapter Ⅲ Highly Sensitive Detection of HBV Based on PCR Amplification and Chemiluminescent1 Detection | 第53-77页 |
| 3.1. Introduction | 第53-55页 |
| 3.2. Materials and Methods | 第55-59页 |
| 3.2.1. Materials | 第55-56页 |
| 3.2.2. Methods | 第56-59页 |
| 3.3. Results and Discussion | 第59-71页 |
| 3.3.1 Polymerase Chain Reaction Amplification | 第59-65页 |
| 3.3.2. Chemiluminescent Detection | 第65-71页 |
| 3.4. Conclusion | 第71-72页 |
| 3.4.1. Designed the primer pair and probe for targeting all genotypes of HBV | 第71-72页 |
| 3.4.2. Established the optimized conditions for HBV genome amplification | 第72页 |
| 3.4.3. Established the best probe and PCR amplicon hybridization conditions | 第72页 |
| 3.4.4 Applied magnetic separation and chemiluminescence technique for establishing the specificity and limit of detection of HBV from serum | 第72页 |
| REFERENCES | 第72-77页 |
| Chapter Ⅳ Magnetic Separation and Chemiluminescence Based Highly Sensitive Method for HCVDetection | 第77-99页 |
| 4.1. Introduction | 第77-79页 |
| 4.2. Materials and Methods | 第79-82页 |
| 4.2.1. Materials | 第79-80页 |
| 4.2.2. Methods | 第80-82页 |
| 4.3. Results and Discussion | 第82-95页 |
| 4.3.1. Polymerase Chain Reaction Amplification | 第82-89页 |
| 4.3.2. Chemiluminescent Detection | 第89-95页 |
| 4.4. Conclusion | 第95-96页 |
| 4.4.1. Designed the primer pair and probe for targeting all genotypes of HCV | 第95页 |
| 4.4.2. Established the optimized conditions for HCV genome amplification | 第95-96页 |
| 4.4.3. Established the best probe and PCR amplicon hybridization conditions | 第96页 |
| 4.4.4. Applied magnetic separation and chemiluminesce technique for establishing the specificity and limit of detection | 第96页 |
| REFERENCES | 第96-99页 |
| Chapter Ⅴ Magnetic Separation and Chemiluminescent Based Highly Sensitive Method for HIVDetection | 第99-112页 |
| 5.1. Introduction | 第99-100页 |
| 5.2. Materials and Methods | 第100-103页 |
| 5.2.1. Materials | 第100页 |
| 5.2.2. Methods | 第100-103页 |
| 5.3. Results and Discussion | 第103-109页 |
| 5.3.1 Polymerase Chain Reaction Amplification | 第103-106页 |
| 5.3.2. Chemiluminescent Detection | 第106-109页 |
| 5.4. Conclusion | 第109-110页 |
| 5.4.1. Designed the primer pair and probe for targeting all group M genotypes of HIV-1 | 第109页 |
| 5.4.2. Established the optimized conditions for HIV genome amplification | 第109页 |
| 5.4.3. Established the best probe and PCR amplicon hybridization conditions | 第109-110页 |
| 5.4.4. Applied magnetic separation and chemiluminesccence technique for establishing the specificity and limit of detection | 第110页 |
| REFERENCES | 第110-112页 |
| Chapter Ⅵ Development of a Chemiluminescence Based Assay for Simultaneous Extraction andDetection of Multiple Viruses | 第112-130页 |
| 6.1. Introduction | 第112-114页 |
| 6.2. Materials and Methods | 第114-117页 |
| 6.2.1. Materials | 第114-115页 |
| 6.2.2. Methods | 第115-117页 |
| 6.3. Results and discussion | 第117-125页 |
| 6.3.1. RT-PCR and gel electrophoresis | 第118-120页 |
| 6.3.2. Chemiluminescent Detection | 第120-125页 |
| 6.4. Conclusion | 第125-126页 |
| 6.4.1. Primer pairs were designed to work in multiplex PCR | 第125页 |
| 6.4.2. Established the optimized conditions for multiplex amplification to achieve higher sensitivity | 第125-126页 |
| 6.4.3 Applied magnetic separation and chemiluminesce technique for establishing the specificity and limit of detection of each virus | 第126页 |
| REFERENCES | 第126-130页 |
| Chapter Ⅶ Semi-Automated System for Multiplex Viral Detection | 第130-142页 |
| 7.1. Introduction | 第130-131页 |
| 7.2. Materials and Methods | 第131-134页 |
| 7.2.1. Materials | 第131-132页 |
| 7.2.2. Methods | 第132-134页 |
| 7.3. Results and Discussion | 第134-139页 |
| 7.3.1. Optimization of MPs related parameters | 第135-136页 |
| 7.3.2. Optimization of Wash Ⅰ | 第136-137页 |
| 7.3.3. Optimization of Wash Ⅱ | 第137-139页 |
| 7.3.4. One step Multiplex RT-PCR and gel electrophoresis | 第139页 |
| 7.4. Conclusion | 第139-140页 |
| 7.4.1. Optimization of parameters for Automated Nucleic Acid extraction | 第139页 |
| 7.4.2. Development of semi-automated system for multiplex detection | 第139-140页 |
| REFERENCES | 第140-142页 |
| Chapter Ⅷ Summary of the Project and Future Work Plan | 第142-144页 |
| 8.1. Summary and conclusions | 第142-143页 |
| 8.2. Directions for Future work | 第143-144页 |
| Appendix | 第144-146页 |
| Acknowledgement | 第146页 |
