| 摘要 | 第5-7页 |
| RESEARCH BACKGROUND | 第12-14页 |
| CHAPTER 1: General introduction and literature review | 第14-29页 |
| 1.1 Introduction | 第14-26页 |
| 1.1.1 Biological strategies for the removal of hydrocarbon pollutants in soil | 第16-24页 |
| 1.1.1.1 Bioremediation | 第16页 |
| 1.1.1.2 Phytoremediation | 第16-18页 |
| 1.1.1.3 Rhizoremediation: Plant assisted microbial degradation | 第18-20页 |
| 1.1.1.4 Optimization of rhizoremediation by bioaugmentation | 第20-21页 |
| 1.1.1.5 Strategies for bioaugmentation | 第21-24页 |
| A. Free culture inoculation | 第21-22页 |
| B. Immobilization of bacterial culture | 第22-24页 |
| 1.1.2 Sesbania cannabina classification and selection criteria | 第24-26页 |
| 1.2 Research objectives | 第26-27页 |
| 1.3 Hypothesis | 第27-29页 |
| CHAPTER 2: Soil texture effects on rhizodegradation of crude oil contaminated soil | 第29-43页 |
| 2.1 Introduction | 第29-30页 |
| 2.2 Materials and Methods | 第30-37页 |
| 2.2.1 Physicochemical analysis of soil sample | 第30-35页 |
| 2.2.1.1 Soil texture | 第30页 |
| 2.2.1.2 Water holding capacity | 第30页 |
| 2.2.1.3 Analysis of moisture content | 第30-31页 |
| 2.2.1.4 Determination of Electrical conductivity | 第31页 |
| 2.2.1.5 Analysis of Cation exchange capacity | 第31页 |
| 2.2.1.6 Determination of total Nitrogen by Kjeldahl’s Method | 第31-32页 |
| 2.2.1.7 Determination of available Nitrogen (NO3-N) by Kjeldahl’s Method | 第32-33页 |
| 2.2.1.8 Determination of available Phosphorus by Olsen P method | 第33页 |
| 2.2.1.9 Analysis of total phosphorous | 第33-34页 |
| 2.2.1.10 Analysis of Soil Organic matter (SOM) | 第34-35页 |
| 2.2.2 Soil Preparation for Pot Experiment | 第35-36页 |
| 2.2.3 Plant cultivation and analysis | 第36页 |
| 2.2.4 Analytical Procedure for TPH concentration | 第36-37页 |
| 2.2.5 Microbial Analysis | 第37页 |
| 2.3 Results and discussion | 第37-42页 |
| 2.3.1 Detection of Crude Oil Degradation | 第37-38页 |
| 2.3.2 Plant growth characteristics | 第38-41页 |
| 2.3.3 MPN analysis | 第41-42页 |
| 2.4 Conclusions | 第42-43页 |
| CHAPTER 3: Effect of plant growth on TPH rhizodegradation in bioaugmented soil | 第43-54页 |
| 3.1 Introduction | 第43-44页 |
| 3.2 Materials and methods | 第44-47页 |
| 3.2.1 Study area | 第44页 |
| 3.2.2 Soil collection and preparation | 第44-45页 |
| 3.2.3 Screening experiments – Seed viability and germination in soil | 第45页 |
| 3.2.4 Pot experiment | 第45-46页 |
| 3.2.5 Plant cultivation and analysis | 第46页 |
| 3.2.6 Statistical analysis | 第46-47页 |
| 3.3 Results and discussion | 第47-53页 |
| 3.3.1 Effect of bioaugmentation on plant growth and root characteristics | 第47-50页 |
| 3.3.2 Correlation of plant growth characteristics with TPH degradation | 第50-53页 |
| 3.4 Conclusions | 第53-54页 |
| CHAPTER 4: Microbial community dynamics in rhizodegradation of bioaugmented TPH contaminated soil | 第54-77页 |
| 4.1 Introduction | 第54-56页 |
| 4.2 Materials and methods | 第56-61页 |
| 4.2.1 Pot experiment | 第56页 |
| 4.2.2 Free oil degrading inoculum preparation | 第56-57页 |
| 4.2.3 Immobilized oil degrading inoculum preparation | 第57页 |
| 4.2.4 Analysis of TPH degraders load | 第57页 |
| 4.2.5 Analysis of Microbial activity | 第57-58页 |
| 4.2.6 Analysis of microbial community and their metabolic profile by Biolog | 第58页 |
| 4.2.7 Analysis of microbial community dynamics by PCR- DGGE technique | 第58-61页 |
| 4.2.7.1 DNA extraction | 第58-60页 |
| 4.2.7.2 DNA quantification by spectrophotometer | 第60页 |
| 4.2.7.3 PCR amplification of extracted DNA | 第60页 |
| 4.2.7.4 DGGE analysis | 第60-61页 |
| 4.2.7.5 Band sequencing and identification | 第61页 |
| 4.2.8 Statistical analysis | 第61页 |
| 4.3 Results and discussion | 第61-76页 |
| 4.3.1 Total oil degrading bacterial load in rhizosphere and bulk soil | 第61-63页 |
| 4.3.2 Microbial activity in rhizosphere and bulk soil | 第63-65页 |
| 4.3.3 Physiological profile of microbial community | 第65-67页 |
| 4.3.4 DNA isolation and quantification | 第67-71页 |
| 4.3.5 PCR- DGGE band analysis and sequencing | 第71-76页 |
| 4.5 Conclusions | 第76-77页 |
| CHAPTER 5: Total petroleum hydrocarbons degradation in bioaugmented soil with free & immobilized consortium | 第77-90页 |
| 5.1 Introduction | 第77-79页 |
| 5.2 Materials and methods | 第79-80页 |
| 5.2.1 Soil preparation for pot experiment | 第79页 |
| 5.2.2 Gas chromatograph-mass spectrometry | 第79-80页 |
| 5.2.3 Statistical analysis | 第80页 |
| 5.3 Results and discussion | 第80-88页 |
| 5.3.1 Effect of bioaugmentation on TPH concentration | 第80-82页 |
| 5.3.2 Comparative analysis of bioaugmented, vegetated and un-vegetated TPH degradation | 第82-84页 |
| 5.3.3 Comparison of plant assisted microbial and indigenous microbial degradation | 第84-88页 |
| 5.4 Conclusions | 第88-90页 |
| CHAPTER 6: General conclusions and research outcomes | 第90-93页 |
| 6.1 Novelty statement | 第91-92页 |
| 6.2 Recommendations | 第92-93页 |
| References | 第93-109页 |
| List of Abbreviations | 第109-111页 |
| Acknowledgment | 第111-112页 |
| Resume | 第112页 |
| Publications | 第112页 |
