| 摘要 | 第7-8页 |
| Abstract | 第8页 |
| Abbreviations | 第12-21页 |
| CHAPTER 1 Introduction and Review of Literature | 第21-35页 |
| 1.1 History | 第21页 |
| 1.2 Plant and Drought | 第21-22页 |
| 1.3 Plant Growth and Productivity | 第22页 |
| 1.4 Drought Effect on Reproductive and Kernel Set | 第22-23页 |
| 1.5 Plant and Water Relations | 第23-32页 |
| 1.5.1 Effect of Drought on Photosynthesis | 第24页 |
| 1.5.2 Physiology of Drought Tolerance | 第24-26页 |
| 1.5.3 Effects of Drought at Cellular Level in Maize | 第26-28页 |
| 1.5.4 Physiological & Molecular Mechanism for Drought Tolerance | 第28-29页 |
| 1.5.5 Cell Elongation & Crop Growth | 第29-30页 |
| 1.5.6 Stomata & photosynthesis | 第30-32页 |
| 1.6 Stress Proteins | 第32-35页 |
| 1.6.1 Cell Membrane Stability | 第33-34页 |
| 1.6.2 Objectives of present study | 第34-35页 |
| CHAPTER 2 The Growth of Transgenic Maize Overexpressing ABP9 under DroughtStress at Vegetative Stage | 第35-55页 |
| 2.1. Summary | 第35页 |
| 2.2 Introduction | 第35-36页 |
| 2.3 Materials and Methods | 第36-38页 |
| 2.3.1 Plant Material and Experimental Conditions | 第36页 |
| 2.3.2 Calculation of chlorophyll fluorescence | 第36-37页 |
| 2.3.3 Calculation of vegetation indices (Normalized Difference VegetationIndex) | 第37页 |
| 2.3.4 Measurement of anthesis silking interval (ASI) | 第37页 |
| 2.3.5 Yield parameters determination | 第37页 |
| 2.3.6 Measurement of soil moisture | 第37-38页 |
| 2.3.7 Statistical Analysis | 第38页 |
| 2.4 Results | 第38-53页 |
| 2.4.1 Phenotype analysis of Pabp9-ABP9.1 transgenic maize under droughtstress at vegetative stage | 第38-42页 |
| 2.4.2 Investigation of phenotypes of Pubi-ABP9.1 transgenic maize underdrought stress at vegetative stage | 第42-46页 |
| 2.4.3 Phenotyping of Pubi-ABP9.2 transgenic maize under drought stress atvegetative stage | 第46-50页 |
| 2.4.4. Phenotyping of Pubi-ABP9-3x FLAG transgenic maize under droughtstress at vegetative stage | 第50-53页 |
| 2.5 Discussion | 第53-55页 |
| CHAPTER 3 The Performance of Transgenic Maize Overexpressing ABP9 underDrought at Reproductive Stage | 第55-76页 |
| 3.1 Summary | 第55页 |
| 3.2 Introduction | 第55-56页 |
| 3.3 Materials and Methods | 第56-58页 |
| 3.3.1 Experimental Conditions | 第56页 |
| 3.3.2 Calculation of chlorophyll fluorescence | 第56-57页 |
| 3.3.3 Calculation of vegetation indices (normalized difference vegetative index) | 第57页 |
| 3.3.4 Estimation of anthesis silking interval (ASI) | 第57页 |
| 3.3.5 Yield parameters determination | 第57页 |
| 3.3.6 Measurement of drought from soil | 第57-58页 |
| 3.4 Result | 第58-74页 |
| 3.4.1 Phenotype investigation of Pabp9-ABP9.1 transgenic maize underdrought stress at reproductive stage | 第58-62页 |
| 3.4.2 Phenotyping of Pubi-ABP9.1 transgenic maize under drought stress atreproductive stage | 第62-66页 |
| 3.4.3 Phenotyping of Pubi-ABP9.2 transgenic maize under drought stress atreproductive stage | 第66-70页 |
| 3.4.4 Phenotyping of Pubi-ABP9-3x FLAG transgenic maize under droughtstress at reproductive stage | 第70-74页 |
| 3.5 Discussion | 第74-76页 |
| CHAPTER 4 Analysis of Transgenic Maize Overexpressing ABP9 under WellWatered Condition | 第76-93页 |
| 4.1 Summary | 第76页 |
| 4.2 Introduction | 第76-77页 |
| 4.3 Materials and Methods | 第77-78页 |
| 4.3.1 Calculation of chlorophyll fluorescence | 第77页 |
| 4.3.2 Calculation of vegetation indices (normalized difference vegetative index) | 第77-78页 |
| 4.3.3 Estimation of anthesis silking interval (ASI) | 第78页 |
| 4.3.4 Yield parameters determination | 第78页 |
| 4.4 Result | 第78-91页 |
| 4.4.1 Phenotyping of Pabp9-ABP9.1 transgenic maize under well watered in field | 第78-82页 |
| 4.4.2 Phenotyping of Pubi-ABP9.1 transgenic maize under well watered in field | 第82-85页 |
| 4.4.3 Phenotyping of Pubi-ABP9.2 transgenic maize under well watered in field | 第85-88页 |
| 4.4.4 Phenotyping of Pubi-ABP9-3x FLAG transgenic maize under well wateredin field | 第88-91页 |
| 4.5 Discussion | 第91-93页 |
| CHAPTER 5 Conclusions | 第93-94页 |
| References | 第94-103页 |
| ACKNOWLEDGEMENTS | 第103-105页 |
| Author Biography | 第105-106页 |
| RESEARCH PUBLICATIONS | 第106-108页 |
