| 摘要 | 第9-11页 |
| Abstract | 第11-13页 |
| Introduction | 第14-17页 |
| Chapter1 REVIEW OF LITERATURE | 第17-43页 |
| 1 SUB ACUTE RUMINAL ACIDOSIS | 第17-24页 |
| 1.1 OCCOURANCE AND EPIDEMIOLOGY | 第19页 |
| 1.2 SARA IN THE EARLY POSTPORTUM PERIOD | 第19-20页 |
| 1.3 SARA IN MID-LACTATION | 第20-21页 |
| 1.4 FEED INTAKE | 第21页 |
| 1.5 ABOMASAL DISPLACEMENT AND ABOMASAL ULCERS | 第21页 |
| 1.6 LAMINITIS | 第21-22页 |
| 1.7 ALTERED MILK COMPOSITION | 第22-23页 |
| 1.8 REPRODUCTION | 第23页 |
| 1.9 RUMEN TYMPANY (BLOAT) | 第23页 |
| 1.10 RUMENITIS | 第23页 |
| 1.11 CAUSES OF RUMEN pH DEPRESSION | 第23-24页 |
| 2 INFLAMMATION | 第24-25页 |
| 3 LIPOPOLYSACCHARIDES (LPS) | 第25-26页 |
| 4 ECONOMIC CONSEQUENCES OF SARA | 第26-27页 |
| 5 MONITORING OF CLINICAL SIGNS | 第27页 |
| 6 NUCLEOTIDE BINDING OLIGOMERIZAION DOMAIN PROTIEN1 (NOD1) | 第27-29页 |
| REFERENCES | 第29-43页 |
| CHAPTER2 THE ACTIVATION OF NF-κB INFLAMMATORY PATHWAY INDUCED BYNOD1 IN THE RUMEN OF DAIRY COWS DURING SARA | 第43-59页 |
| 1. INTRODUCTION | 第45-47页 |
| 2. MATERIAL AND METHODS | 第47-51页 |
| 2.1 ETHICS STATEMENT | 第47页 |
| 2.2 ANIMALS,DIETS AND EXPERIMENTAL DESIGN | 第47页 |
| 2.3 COLLECTION OF SAMPLES | 第47-48页 |
| 2.4 MEASUREMENT OF RUMINAL pH AND LPS CONCENTRATION IN THE RUMEN FLUID | 第48-49页 |
| 2.5 RADIO IMMUNOASSAY | 第49页 |
| 2.6 REAL TIME (RT-qPCR) | 第49-50页 |
| 2.7 STATISTICAL ANALYSES | 第50-51页 |
| 3. RESULTS | 第51-54页 |
| 3.1 ALTERATIONS IN THE RUMEN pH | 第51页 |
| 3.2 DETERMINATION OF LPS IN RUMEN FLUID OF DAIRY COWS | 第51-52页 |
| 3.3 THE CONCENTRATION OF PROINFLAMMATORY CYTOKINES INPERIPHERAL BLOOD OF DAIRY COWS FROM HC AND LC GROUP | 第52-53页 |
| 3.4 THE mRNAs EXPRESSION OF PROINFLAMMATORY CYTOKINES IN THERUMEN OF DAIRY COWS | 第53页 |
| 3.5 THE mRNAs EXPRESSION OF IMMUNE GENES IN THE RUMEN TISSUEOF DAIRY COWS | 第53-54页 |
| 4. DISCUSSION | 第54-56页 |
| REFERENCES | 第56-59页 |
| CHAPTER3 ACTIVATION OF NOD1 AND EXPRESSIONS OF PROINFLAMMATORYCYTOKINS IN THE RUMEN OF LACTATING GOATS DURING SARA | 第59-73页 |
| 1. INTRODUCTION | 第60-61页 |
| 2. MATERIAL AND METHODS | 第61-65页 |
| 2.1 ETHICS STATEMENT | 第61页 |
| 2.2 ANIMALS,DIETS,AND EXPERIMENTAL DESIGN | 第61页 |
| 2.3 SAMPLE COLLECTION | 第61-63页 |
| 2.4 MEASUREMENT OF pH AND LPS CONCENTRATION IN THE RUMEN FLUID | 第63页 |
| 2.5 RADIO IMMUNOASSAY | 第63页 |
| 2.6 REAL TIME (RT-qPCR) | 第63-64页 |
| 2.7 STATISTICAL ANALYSES | 第64-65页 |
| 3. RESULTS | 第65-69页 |
| 3.1 VARIATIONS IN RUMINAL pH OF LACTATING GOATS | 第65页 |
| 3.2 DETERMINATION OF LPS IN RUMEN FLUID | 第65-66页 |
| 3.3 CONCENTRATION OF PPRO-INFLAMMATORY CYTOKINES INPERIPHERAL BLOOD OF LACTATING GOATS | 第66页 |
| 3.4 THE mRNAs EXPRESSION OF PRO-INFLAMMATORY CYTOKINES INTHE RUMEN TISSUE OF LACTATING GOATS | 第66-67页 |
| 3.5 The mRNAs EXPRESSION OF IMMUNE GENES IN THE RUMEN TISSUEOF LACTATING GOATS | 第67-68页 |
| 4. DISCUSSION | 第68-69页 |
| REFERENCES | 第69-73页 |
| CHAPTER4 NOD1/NF-κB SIGNALING PATHWAY INHIBITED BY BUFFER IN THEMAMMARY GLAND OF LACTATING GOATS DURING SARA | 第73-89页 |
| 1. INTRODUCTION | 第74-76页 |
| 2. MATERIAL AND METHODS | 第76-79页 |
| 2.1 ETHICS STATEMENT | 第76页 |
| 2.2 ANIMALS, DIETS AND EXPERIMENTAL DESIGN | 第76页 |
| 2.3 SAMPLE COLLECTION | 第76-77页 |
| 2.4 MEASUREMENT OF RUMINAL pH AND LPS IN PERIPHERAL BLOOD | 第77页 |
| 2.5 RADIO IMMUNOASSAY | 第77-78页 |
| 2.6 REAL TIME (RT-qPCR) | 第78页 |
| 2.7 STATISTICAL ANALYSES | 第78-79页 |
| 3. RESULTS | 第79-82页 |
| 3.1 ALTERATION IN THE RUMEN pH | 第79页 |
| 3.2 LPS DETERMINATION | 第79-80页 |
| 3.3 THE CONCENTRATION OF PRO-INFLAMMATORY CYTOKINES INLACTEAL BLOOD | 第80-81页 |
| 3.4 THE mRNAs EXPRESSION OF PROINFLAMMATROY CYTOKINES IN THEMAMMARY GLAND OF LACTATING GOATS | 第81页 |
| 3.5 THE mRNAs EXPRESSION OF IMMUNE GENES IN THE MAMMARYGLAND OF LACTATING GOATS | 第81-82页 |
| 4. DISCUSSION | 第82-84页 |
| REFERENCES | 第84-89页 |
| SUPPLEMENTARY MATERIAL | 第89-91页 |
| ACKNOWLEDGMENT | 第91页 |
