| Abstract | 第5-6页 |
| 中文摘要 | 第7-8页 |
| Abbreviations | 第8-10页 |
| Chapter Ⅰ:Review Hedgehog signaling pathway | 第10-49页 |
| 1 Hedgehog signal transduction in Drosophila | 第11-25页 |
| 1.1 Hedgehog | 第12-14页 |
| 1.2 Patched | 第14-15页 |
| 1.3 Smoothened | 第15-17页 |
| 1.4 Cubitus interruptus | 第17-19页 |
| 1.5 Suppressor of Fused | 第19-22页 |
| 1.6 Hh-induced BTB protein | 第22-24页 |
| 1.7 The Hh signaling complex | 第24-25页 |
| 2. Hedgehog signal transduction in vertebrate | 第25-34页 |
| 2.1 The conservation and divergence of Hedgehog pathway from Drosophila to vertebrate | 第26-30页 |
| 2.2 SUFU in mammalian | 第30-32页 |
| 2.3 Speckle-type POZ protein in mammalian | 第32-34页 |
| 3 Hedgehog signaling and diseases | 第34-38页 |
| References | 第38-49页 |
| Chapter Ⅱ:Hedgehog signaling downre:gulates Suppressor of Fused through theHIB/SPOP-Crn axis in Drosophila | 第49-91页 |
| Abstract | 第50页 |
| Introduction | 第50-52页 |
| Materials and Methods | 第52-57页 |
| Results | 第57-83页 |
| Section 2.1 Hh signaling downregulates Su(fu) | 第57-59页 |
| Section 2.2 Hh signaling downregulates Su(fu)through its target HIB | 第59-61页 |
| Section 2.3 Cul3-HIB downregulates Su(fu)through the ubiquitin/proteasome pathway | 第61-65页 |
| 2.3.1 Regulation of Su(fu)level by HIB depends on E3 ligase Cul3 | 第62-63页 |
| 2.3.2 The ubiquitin/proteasome pathway is required for Su(fu)downregulation | 第63-65页 |
| Section 2.4 HIB does not directly regulate Su(fu) | 第65-70页 |
| 2.4.1 Su(fu)stability is not regulated by HIB either in S2 cells or in wing discs | 第65-67页 |
| 2.4.2 HIB does not affect the stability of phosphorylated Su(fu)either in S2 cells or in wing discs | 第67-69页 |
| 2.4.3 HIB does not affect su(fu)mRNA either in S2 cells or in wing discs | 第69-70页 |
| Section 2.5 HIB affects Su(fu)level through Crn | 第70-73页 |
| Section 2.6 HIB reduces Su(fu)level by modulating Cm subcellular localization | 第73-79页 |
| 2.6.1 HIB-mediated downregulation of Su(fu) is through modulating Crn subcellular localization in S2 cells | 第73-75页 |
| 2.6.2 HIB also affects the translocation of Crn in wing discs | 第75-77页 |
| 2.6.3 HIB does not affect su(fu)mRNA translocation through Crn | 第77页 |
| 2.6.4 HIB downregulates Su(fu)through regulating its translation | 第77-79页 |
| Section 2.7 Hh-HIB-Crn downregulates Su(fu)level in S2 cells | 第79-80页 |
| Section 2.8 Su(fu)affects Hh signaling in a genetically sensitized background | 第80-81页 |
| Section 2.9 SPOP can regulate Su(fu)in Drosophila | 第81-83页 |
| Discussion | 第83-87页 |
| References | 第87-91页 |
| 致谢 | 第91-92页 |
| Publication | 第92-94页 |
