| 摘要 | 第7-8页 |
| abstract | 第8页 |
| CHAPTER 1 Introduction | 第12-17页 |
| 1.1 Leaf senescence | 第12-16页 |
| 1.2 Objective | 第16-17页 |
| CHAPTER 2 Materials and Methods | 第17-26页 |
| 2.1 Plant materials | 第17-18页 |
| 2.2 Agronomic traits assessment | 第18页 |
| 2.3 Genotyping with SSR and In Del markers | 第18页 |
| 2.4 PCR cocktail for genotyping | 第18-19页 |
| 2.5 RNA isolation and real-time PCR | 第19-23页 |
| 2.5.1 RNA extraction method | 第20-21页 |
| 2.5.2 Reverse c DNA for Real time PCR using Rever Tra Ace? q PCR RT Master Mix with g DNA Remover (Toyobo, Japan) | 第21-22页 |
| 2.5.3 Real time PCR reaction cocktail using SYBR? Premix Ex TaqTM II | 第22-23页 |
| 2.6 Histochemistrical determination | 第23-24页 |
| 2.7 Stress response | 第24页 |
| 2.8 Chlorophyll and anthocyanin measurements | 第24页 |
| 2.9 Characterization of chloroplast ultrastructure using transmission electron microscope | 第24-25页 |
| 2.10 Determination of sugar content,Hydrogen peroxide (H_2O_2), malondialdehyde (MDA), protein content,catalase activity (CAT), total antioxidant capability (T-AOC) and superoxide dismutase activity (SOD) | 第25页 |
| 2.11 Photosynthetic rate measurement | 第25-26页 |
| CHAPTER 3 Characterization and mapping of es2 | 第26-40页 |
| 3.1 Results | 第26-36页 |
| 3.1.1 Physiological appearance in es2 mutant line | 第26-28页 |
| 3.1.2 Senescence-associated genes (SAGs) were up-regulated in es2 | 第28-29页 |
| 3.1.3 Chlorophyll content under dark-induced senescence circumstance | 第29-30页 |
| 3.1.4 Chloroplast ultrastructure was altered in es2 | 第30-31页 |
| 3.1.5 Net photosynthetic rate had decreased in es2 | 第31-32页 |
| 3.1.6 Early senescence affected yield in es2 | 第32-33页 |
| 3.1.7 Histochemical staining of wild type and es2 | 第33-34页 |
| 3.1.8 Assessment of oxidative stress and byproducts in es2 | 第34-35页 |
| 3.1.9 Preliminary mapping of es2 | 第35-36页 |
| 3.2 Discussion | 第36-38页 |
| 3.3 Conclusion | 第38-40页 |
| Chapter 4 Physiological identification of g1029 | 第40-52页 |
| 4.1 Result | 第40-48页 |
| 4.1.1 Characteristics appearance of g1029 | 第40-41页 |
| 4.1.2 Determination of sugar content in g1029 | 第41-42页 |
| 4.1.3 Determination of oxidative stress and by-products in g1029 | 第42-43页 |
| 4.1.4 Histochemical staining | 第43-44页 |
| 4.1.5 Mesophyll ultrastructure | 第44-45页 |
| 4.1.6 Agronomic traits were reduced in g1029 | 第45-46页 |
| 4.1.7 qRT-PCR of the related SAGs in g1029 | 第46-48页 |
| 4.2 Discussion | 第48-51页 |
| 4.3 Conclusion | 第51-52页 |
| References | 第52-57页 |
| Acknowledgement | 第57-59页 |
| Author's Curriculum Vitae | 第59-60页 |
