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水稻早衰突变体es2和g1029的鉴定以及es2基因的初定位

摘要第7-8页
abstract第8页
CHAPTER 1 Introduction第12-17页
    1.1 Leaf senescence第12-16页
    1.2 Objective第16-17页
CHAPTER 2 Materials and Methods第17-26页
    2.1 Plant materials第17-18页
    2.2 Agronomic traits assessment第18页
    2.3 Genotyping with SSR and In Del markers第18页
    2.4 PCR cocktail for genotyping第18-19页
    2.5 RNA isolation and real-time PCR第19-23页
        2.5.1 RNA extraction method第20-21页
        2.5.2 Reverse c DNA for Real time PCR using Rever Tra Ace? q PCR RT Master Mix with g DNA Remover (Toyobo, Japan)第21-22页
        2.5.3 Real time PCR reaction cocktail using SYBR? Premix Ex TaqTM II第22-23页
    2.6 Histochemistrical determination第23-24页
    2.7 Stress response第24页
    2.8 Chlorophyll and anthocyanin measurements第24页
    2.9 Characterization of chloroplast ultrastructure using transmission electron microscope第24-25页
    2.10 Determination of sugar content,Hydrogen peroxide (H_2O_2), malondialdehyde (MDA), protein content,catalase activity (CAT), total antioxidant capability (T-AOC) and superoxide dismutase activity (SOD)第25页
    2.11 Photosynthetic rate measurement第25-26页
CHAPTER 3 Characterization and mapping of es2第26-40页
    3.1 Results第26-36页
        3.1.1 Physiological appearance in es2 mutant line第26-28页
        3.1.2 Senescence-associated genes (SAGs) were up-regulated in es2第28-29页
        3.1.3 Chlorophyll content under dark-induced senescence circumstance第29-30页
        3.1.4 Chloroplast ultrastructure was altered in es2第30-31页
        3.1.5 Net photosynthetic rate had decreased in es2第31-32页
        3.1.6 Early senescence affected yield in es2第32-33页
        3.1.7 Histochemical staining of wild type and es2第33-34页
        3.1.8 Assessment of oxidative stress and byproducts in es2第34-35页
        3.1.9 Preliminary mapping of es2第35-36页
    3.2 Discussion第36-38页
    3.3 Conclusion第38-40页
Chapter 4 Physiological identification of g1029第40-52页
    4.1 Result第40-48页
        4.1.1 Characteristics appearance of g1029第40-41页
        4.1.2 Determination of sugar content in g1029第41-42页
        4.1.3 Determination of oxidative stress and by-products in g1029第42-43页
        4.1.4 Histochemical staining第43-44页
        4.1.5 Mesophyll ultrastructure第44-45页
        4.1.6 Agronomic traits were reduced in g1029第45-46页
        4.1.7 qRT-PCR of the related SAGs in g1029第46-48页
    4.2 Discussion第48-51页
    4.3 Conclusion第51-52页
References第52-57页
Acknowledgement第57-59页
Author's Curriculum Vitae第59-60页

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