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基于小环DNA的核酸自组装技术用于合成一维和二维纳米结构

中文摘要第7-9页
Abstract第9-10页
Chapter 1. Introduction第11-31页
    1.1 DNA Nanotechnology第11-12页
    1.2 DNA Self-assembly第12-13页
    1.3 Role of DNA as a material第13页
    1.4 DNA tiles第13-15页
        1.4.1 Holliday Junction第15页
    1.5 Self-assembly of DNA nanotubes第15-17页
    1.6 Self-assembly of 2D DNA lattices第17-18页
        1.6.1 2D DNA lattices based on Mao's tensegrity triangle tile第17-18页
    1.7 Self-assembly of 3D DNA lattices第18-22页
        1.7.1 Post-assembly stabilization of 3D DNA crystals第20-22页
    1.8 DNA origami第22-24页
    1.9 Self-assembly of complex shapes from single-stranded DNA tiles (SST)第24-26页
    References第26-31页
Chapter 2. How small DNA minicircles can be applied to construct DNANanotubes第31-49页
    2.1 Abstract第31页
    2.2 Introduction第31-33页
    2.3 Results and Discussion第33-40页
        2.3.1 Preparation of circular building-blocks第33页
        2.3.2 Design principle of NT-1 and NT -2 DNA nanotubes and their AFMimages第33-36页
        2.3.3 Characterization and AFM images of NT-3 DNA nanotubes第36-38页
        2.3.4 Characterization and AFM images of NT-4 DNA nanotubes第38-39页
        2.3.5 Branching mechanism of nanotubes第39-40页
    2.4 Experimental Section第40-46页
        2.4.1 Materials第40页
        2.4.2 Methods and Instruments第40-41页
        2.4.3 Circularization of linear 5'-phosphorylated oligonucleotides第41页
        2.4.4 Denaturing PAGE analysis of the purified circular DNAs第41-42页
        2.4.5 Self-Assembly of circular DNAs to create robust nanotubes第42-43页
        2.4.6 Designs of motifs comprising nanotubes第43-44页
        2.4.7 More AFM images of nanotubes NT1-NT4第44-45页
        2.4.8 Sequences used in the text第45-46页
    2.5 Conclusion第46页
    Reference第46-49页
Chapter 3. DNA nanostructures assembled from tensegrity triangle tiles withcircular DNA scaffolds第49-79页
    3.1 Abstract第49页
    3.2 Introduction第49-51页
    3.3 Results and Discussion第51-59页
        3.3.1 Nomenclature of nanotubes第51-52页
        3.3.2 Folding scheme of triangle tiles第52-53页
        3.3.3 Schematics of parallel lozenge tiling lattice第53-54页
        3.3.4 Characterization and AFM images of NT-1第54-55页
        3.3.5 Characterization and AFM images of NT-2 & NT-5第55页
        3.3.6 Characterization and AFM images of NT-3,NT -4 & NT -6第55-56页
        3.3.7 Characterization and AFM images of NT-7 to NT-9第56-57页
        3.3.8 Comprehensive characterization of NT-1 to NT-9第57-58页
        3.3.9 Limits of design principle第58-59页
    3.4 Experimental Section第59-76页
        3.4.1 Materials第59页
        3.4.2 Preparation of circularized DNA第59-60页
        3.4.3 Purification of circularized DNA via denaturing PAGE第60页
        3.4.4 Synthesis of self-assembled nanostructures第60页
        3.4.5 Native polyacrylamide gel electrophoresis (PAGE)第60-61页
        3.4.6 Qualitative statistics of production yield of nanotubes第61页
        3.4.7 Atomic force microscopy (AFM)第61-62页
        3.4.8 Additional AFM images of NT1-NT9第62-63页
        3.4.9 AFM images of a control experiment第63页
        3.4.10 Graphical explanation of cross-sectional profile of DNA nanotubes第63-69页
        3.4.11 Designs of DNA triangle tiles forming nanotubes第69-74页
        3.4.12 Oligonucleotide sequences used in the text第74-76页
    3.5 Conclusion第76页
    Reference第76-79页
Summary第79-82页
Innovation第82-83页
Publications第83-84页
Acknowledgement第84-85页

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