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利用密旋链霉菌和生物炭对工矿业污染土壤植物修复及降低重金属毒性的研究

Abstract第6-8页
摘要第9-20页
CHAPTER 1. INTRODUCTION AND REVIEW OF LITERATURE第20-51页
    1.1 Potentially toxic trace elements (PTEs)第20-21页
    1.2 Sources of potential toxic trace elements (PTEs) in the environment第21-24页
    1.3 Global overview of PTEs pollution第24-26页
    1.4 Chinese scenario of soil pollution第26-28页
    1.5 Detrimental impacts of PTEs on public health and plants第28-33页
        1.5.1 Zinc (Zn)第29页
        1.5.2 Lead (Pb)第29-30页
        1.5.3 Cadmium (Cd)第30-31页
        1.5.4 Copper (Cu)第31页
        1.5.5 Nickle (Ni)第31-32页
        1.5.6 Mercury (Hg)第32页
        1.5.7 Arsenic (As)第32页
        1.5.8 Chromium (Cr)第32-33页
        1.5.9 Aluminum (Al)第33页
    1.6 Soil remediation techniques第33-36页
    1.7 Phytoremediation and its importance第36-39页
    1.8 Microbes assisted phytoremediation第39-41页
    1.9 Streptomyces pactum for phytoremediation: A novel soil microorganism第41-47页
        1.9.1 Fate of plants used in the phytoremediation of PTEs第42-43页
        1.9.2 Immobilization of PTEs in polluted soil using organic amendments第43-44页
        1.9.3 The nature of biochar and its potential to adsorb PTEs第44-46页
        1.9.4 Limitations of biochar as soil amendment第46-47页
    1.10 Effect of PTEs on enzyme activity第47-50页
        1.10.1 Soil enzymatic activities affected by PTEs第47-49页
        1.10.2 Plant antioxidant activities as function of soil contamination第49-50页
    1.11 Research objectives第50-51页
CHAPTER 2. MATERIALS AND METHODS第51-64页
    2.1 Sites Description第51-52页
        2.1.1 Tongguan (TG)第51页
        2.1.2 Feng County (FC)第51-52页
        2.1.3 Chenzhou (CZ)第52页
    2.2 Materials Collection第52-54页
        2.2.1 Collection of contaminated soil samples第52-53页
        2.2.2 Isolation of Streptomyces pactum第53页
        2.2.3 Raw materials for composting第53-54页
        2.2.4 Biochar collection第54页
    2.3 Experimental Methods第54-63页
        2.3.1 Soil, compost and biochar analysis第54-58页
        2.3.2 Plant analysis第58-63页
            2.3.2.4 Determination of total PTEs content in plant samples第59页
            2.3.2.5 Determination of antioxidant enzymatic activities in plant第59-62页
            2.3.2.6 Measurement of MDA第62-63页
            2.3.2.7 Phytoextraction indices第63页
    2.4 Quality control and statistical analysis第63-64页
CHAPTER 3. ROLE OF STREPTOMYCES PACTUM IN PHYTOREMEDIATION OF TRACE ELEMENTS IN MINE POLLUTED SOILS OF SHAANXI, NORTHWEST CHINA第64-82页
    3.1 Introduction第64-65页
    3.2 Materials and Methods第65-67页
        3.2.1 Samples collection第65页
        3.2.2 Experiment Methods第65-67页
    3.3 Results and Discussion第67-81页
        3.3.1 Characteristics of the studied soils and medical stone compost第67-69页
        3.3.2 Effect of Streptomyces pactum on PTEs uptake by Brassica juncea第69-74页
        3.3.3 Effect of Streptomyces pactum on growth attributes of Brassica juncea第74-77页
        3.3.4 Effect of Streptomyces pactum on leaf antioxidant activities第77-79页
        3.3.5 Phytoextraction indices of trace elements第79-81页
    3.4 Conclusions第81-82页
CHAPTER 4. USING BAMBOO BIOCHAR WITH COMPOST FOR STABILIZATION AND PHYTOTOXICITY REDUCTION OF TRACE ELEMENTS IN MINESCONTAMINATED SOILS OF SHAANXI PROVINCE, CHINA第82-102页
    4.1 Introduction第82-84页
    4.2 Materials and Methods第84-86页
        4.2.1 Materials collection第84页
        4.2.2 Experimental Methods第84-86页
    4.3 Results and Discussion第86-101页
        4.3.1 Characteristics of studied soils, medical stone compost and bamboo biochar第86-88页
        4.3.2 Effect of biochar amendments on soil pH and EC第88-89页
        4.3.3 Effect of biochar amendments on PTEs bioavailability第89-90页
        4.3.4 Effect of biochar on PTEs translocation in Brassica juncea shoot第90-92页
        4.3.5 Effect of biochar on PTEs accumulation in Brassica juncea root第92-93页
        4.3.6 Phytotoxicity assay of Brassica juncea in polluted soils第93-96页
        4.3.7 Effect of biochar on the soil enzymatic activities第96-97页
        4.3.8 Effect of biochar on the plant antioxidant enzymes第97-100页
        4.3.9 Phytoextraction indices of PTEs第100-101页
    4.4 Conclusions第101-102页
CHAPTER 5. INTEGRATED EFFECT OF STREPTOMYCES PACTUM (ACT12) AND BIOCHAR ON THE PHYTOEXTRACTION OF POTENTIAL TOXIC TRACEELEMENTS IN CONTAMINATED SOILS第102-129页
    5.1 Introduction第102-104页
    5.2 Materials and Methods第104-105页
        5.2.1 Materials collection and Act12 isolation第104页
        5.2.2 Experimental methods第104-105页
    5.3 Results and Discussion第105-127页
        5.3.1 Physico-chemical characteristics of soil, compost and biochar第105-108页
        5.3.2 Effect of biochar and Act12 on soil pH and EC第108-110页
        5.3.3 Effect of biochar and Act12 on bioavailable fraction of PTEs第110-111页
        5.3.4 Effect of biochar and Act12 on phytoextraction of PTEs in sorghum shoots第111-115页
        5.3.5 Effect of biochar and Act12 on phytoextraction of PTEs in sorghum root第115-117页
        5.3.6 Plant growth promotion by biochar and Act12第117-120页
        5.3.7 Effect of biochar and Act 12 on the soil enzymatic activities第120-123页
        5.3.8 Effect of biochar and Act12 on the plant anti-enzymatic activities第123-124页
        5.3.9 Lipid peroxidation product第124-125页
        5.3.10 Phytoextraction indices of PTEs第125-127页
    5.4 Conclusions第127-129页
SUMMARY AND MAIN CONCLUSIONS第129-131页
SUGGESTIONS AND RECOMMENDATIONS第131-132页
REFERENCES第132-150页
NOMENCLATURE第150-152页
ABOUT THE AUTHOR第152-153页
PUBLISHED PEER REVIEWED PAPERS (SCI)第153-156页

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