| ABBREVIATIONS | 第9-10页 |
| ABSTRACT | 第10-11页 |
| CHAPTER 1 REVIEW | 第12-34页 |
| 1 STRESS | 第12-14页 |
| 2 HEAT SHOCK PROTEINS | 第14-17页 |
| 2.1. Highly conserved | 第17页 |
| 2.2. Wide spread availabilty in all organisms | 第17页 |
| 2.3. Non specificity in nature | 第17页 |
| 2.4. Other Features of HSPs | 第17页 |
| 3 REGULATION OF HSPs | 第17-19页 |
| 4 STRESS DAMAGES AND HSPs PROTECTION IN MYOCARDIAL CELLS | 第19-21页 |
| 4.1 Stress damage cause mortality in vivo | 第19页 |
| 4.2 Myocardial cell damage and enzyme elevations | 第19页 |
| 4.3 Regulation of Apoptosis | 第19-20页 |
| 4.4 Hsps and cell protection | 第20-21页 |
| 5 HSP60 | 第21-24页 |
| REFERENCES | 第24-34页 |
| CHAPTER 2 Stress damages of myocardial cells ofneonatal rats in vitro after heat stress | 第34-42页 |
| Abstract | 第34页 |
| 1 Introduction | 第34-35页 |
| 2 Materials and Methods | 第35-37页 |
| 2.1 Cell culture and heat treatment | 第35页 |
| 2.2 Tests of the activities of LDH,AST,CK,and CK-MB | 第35页 |
| 2.3 Treatment of coverslips with polylysine solution | 第35-36页 |
| 2.4 Growth of cells on coverslip | 第36页 |
| 2.5 Fixation of cells | 第36页 |
| 2.6 Hematoxylin-Eosin (H&E)staining | 第36-37页 |
| 2.7 Statistical analysis | 第37页 |
| 3 Results | 第37-39页 |
| 3.1 The enzyme levels AST,LDH,CK and CK-MB in the cultured cellular supernatant | 第37-39页 |
| 3.2 Pathological Lesions of the heat stressed myocardial cells | 第39页 |
| 4 Discussion | 第39-42页 |
| CHAPTER 3 Localization and expression of Hsp60 in the heat stressed primarymyocardial cells of neonatal rats in vitro | 第42-52页 |
| Abstract | 第42页 |
| 1 Introduction | 第42-44页 |
| 2 Materials and methods | 第44-47页 |
| 2.1 Cell culture and heat stress treatment | 第44页 |
| 2.2 Immunocytochemistry | 第44-45页 |
| 2.3 Western blotting | 第45页 |
| 2.4 Treatment of coverslips with polylysine solution | 第45-46页 |
| 2.5 Growth of cells on coverslip | 第46页 |
| 2.6 Fixation of cells | 第46页 |
| 2.7 Hematoxylin-Eosin (H&E) staining | 第46-47页 |
| 2.8 Statistical analysis | 第47页 |
| 3 Results | 第47-49页 |
| 3.1 The localization and distribution of Hsp60 in the myocardial cells in vitro | 第47页 |
| 3.2 Quantitative detection of Hsp60 expression in the heat-stressed myocardial cells ofneonatal rats in vitro | 第47页 |
| 3.3 Cytopathogical lesions in the heat stressed myocardial cells of rats in vitro | 第47-49页 |
| 4 Discussion | 第49-52页 |
| CHAPTER 4 Influences of Hsf-1 and its corresponding mRNA on the expression ofHsp60 and its corresponding mRNA in heat stressed myocardial cells of rats in vitro | 第52-62页 |
| Abstract | 第52页 |
| 1 Introduction | 第52-54页 |
| 2 Materials and methods | 第54-56页 |
| 2.1 Cell culture and experimental treatment | 第54页 |
| 2.2 Western blotting | 第54页 |
| 2.3 Detection of hsp60 mRNA by lfuorescence quantitative real-time polymerase chainreaction (FQ RT-PCR) | 第54-56页 |
| 2.4 Statistical analysis | 第56页 |
| 3 Results | 第56-59页 |
| 3.1 Quantitative detection of Hsp60 expression in the heat-stressed myocardial cells of neonatal rats in vitro | 第56页 |
| 3.2 Quantitative detection of Hsf-1 expression in the heat-stressed myocardial cells of neonatal rats in vitro | 第56-57页 |
| 3.3 The transcription levels of hsp60 mRNA in the heat-stressed myocardial cells of neonatal rats in vitro | 第57页 |
| 3.4 The transcription levels of hsf-1 mRNA in the heat-stressed myocardial cells of neonatal rats in vitro | 第57-59页 |
| 4 Discussion | 第59-62页 |
| CHAPTER 5 Role of Hsp60 in preventing myocardial cells of neonatal rats againstapoptosis during heat stress | 第62-70页 |
| 1 Introduction | 第62-64页 |
| 2 Materials and Methods | 第64-65页 |
| 2.1 Cell culture and experimental treatment | 第64页 |
| 2.2 Western blotting | 第64页 |
| 2.3 Determination of Cytochrome-C,Caspase-3,Caspase-8 and Caspase-9 | 第64-65页 |
| 2.4 Statistical analysis | 第65页 |
| 3 Results | 第65-68页 |
| 3.1 Semi-quantitative detection of Hsp60 expression in the heat-stressed myocardial cells of neonatal rats in vitro | 第65页 |
| 3.2 The expression of Cleaved Caspase-3 in the cultured myocardial cells in vitro | 第65-66页 |
| 3.3 The levels of Cytochrome-c in the cultured myocardial cells in vitro | 第66页 |
| 3.4 The levels of cleaved Caspase-8 in the cultured myocardial cells in vitro | 第66页 |
| 3.5 The levels of cleaved Caspase-9 in the cultured myocardial cells in vitro | 第66-68页 |
| 4 Discussion | 第68-70页 |
| REFERENCES | 第70-82页 |
| CONCLUSION | 第82-84页 |
| INNOVATION | 第84-86页 |
| ACKNOWLEDGEMENTS | 第86-88页 |
| PUBLICATIONS | 第88页 |
