| Acknowledgements | 第5-6页 |
| Abstract | 第6页 |
| List of abbreviations | 第9-10页 |
| Chapter 1 Introduction and background | 第10-23页 |
| 1.1 Structure: natural products containing sulfonamide group | 第10-14页 |
| 1.2 Biosynthesis:mechanisms of N-S bond formation | 第14-18页 |
| 1.3 Bioactivity: aminoacyl tRNA synthetases inhibition | 第18-21页 |
| 1.4 Aims of this study | 第21-22页 |
| We will do | 第22-23页 |
| Chapter 2 Identification of the biosynthetic gene cluster | 第23-27页 |
| 2.1 Materials and methods | 第23-25页 |
| 2.2 Results and discussions | 第25-27页 |
| Chapter 3 Heterologous expression of the sbz cluster | 第27-39页 |
| 3.1 Materials and methods | 第27-36页 |
| 3.2 Results and discussions | 第36-39页 |
| 3.2.1 The heterologous expression result | 第36-37页 |
| 3.2.2 Structure elucidation of the metabolites | 第37-39页 |
| Chapter 4 Enzymes involved in aminoacyl transfer reactions | 第39-61页 |
| 4.1 Materials and methods | 第39-44页 |
| 4.2 Results and discussions | 第44-60页 |
| 4.2.1 Enzyme involved in N-acyl transfer of L-isoleucine | 第44-49页 |
| 4.2.2 Enzymes involved in O-acyl transfer of β-methylphenylalanine | 第49-56页 |
| 4.2.3 Enzymes involved in N-acyl transfer of 2-sulfamoylacetic acid | 第56-60页 |
| 4.3 Summary | 第60-61页 |
| Chapter 5 Enzymes involved in biogenesis of sulfonamide | 第61-85页 |
| 5.1 Materials and methods | 第61-67页 |
| 5.2 Results and discussions | 第67-85页 |
| 5.2.1 Genes involved in sulfonamide biosynthesis | 第67-68页 |
| 5.2.2 Precursor of sulfonamide | 第68-70页 |
| 5.2.3 rotein expression of SbzM and SbzJ | 第70页 |
| 5.2.4 In vitro reconstitution of 5 | 第70-71页 |
| 5.2.5 Elucidation the pathway from L-cysteine to 5 | 第71-76页 |
| 5.2.6 Tracing the origin of oxygen | 第76-77页 |
| 5.2.7 Biochemical properties of SbzM | 第77-80页 |
| 5.2.8 Proposed mechanism of SbzM | 第80-85页 |
| Chapter 6 Conclusions | 第85-87页 |
| References | 第87-94页 |
| Appendix | 第94-105页 |
| Vita | 第105-106页 |
