| Abstract | 第6页 |
| Abbreviations | 第7-8页 |
| 1. Introduction | 第8-28页 |
| 1.1 Plant immunity | 第8页 |
| 1.2 Cotton and Verticillium dahliae | 第8-10页 |
| 1.3 Research progress to control Verticillium wilt in cotton | 第10-17页 |
| 1.3.1 Research on traditional breeding approaches | 第10-12页 |
| 1.3.2 Research on non-Conventional approaches | 第12-13页 |
| 1.3.3 Research on Genetie Engineering approaches | 第13页 |
| 1.3.4 Research on Genetics and Molecular markers | 第13-14页 |
| 1.3.5 Research on resistant gene cloning | 第14-16页 |
| 1.3.6 Important metabolisms/signal pathways of cotton involved in resistance to Verticillium wilt | 第16-17页 |
| 1.4 Lipoxygenase family in plants | 第17-27页 |
| 1.4.1 LOXs structure,function and occurrence | 第18-19页 |
| 1.4.2 Substrates for LOXs | 第19页 |
| 1.4.3 The metabolism pathway by LOX | 第19-20页 |
| 1.4.4 Functions of LOXs metabolites | 第20-22页 |
| 1.4.5 Role of Lipoxygenases in biotic and abiotic stresses | 第22-27页 |
| 1.5 Aims of present study | 第27-28页 |
| 2. Material and methods | 第28-34页 |
| 2.1 Vector construction and plant transformation | 第28页 |
| 2.2 Isolation and Characterization of LOXs genes | 第28页 |
| 2.3 The Subcellular localization | 第28-29页 |
| 2.4 Cotton transformation and growth of transgenic plants | 第29-30页 |
| 2.5 DNA extraction and PCR for the selection of positive plants | 第30页 |
| 2.6 Gene expression analysis | 第30-31页 |
| 2.7 Southern blotting | 第31-32页 |
| 2.8 Material and methods for VIGS experiments | 第32-34页 |
| 2.8.1 Plant material and growth condition | 第32页 |
| 2.8.2 Fungus preparation and inoculation | 第32-33页 |
| 2.8.3 Fungal recovery assay | 第33-34页 |
| 3. Results | 第34-50页 |
| 3.1 Cloning and Sequence analysis of GhLOX2 and GhLOX5 | 第34-38页 |
| 3.2 Expression pattera analysis of GhLOX2 & GhLOX5 | 第38-40页 |
| 3.2.1 Organ specific expression pattern | 第38页 |
| 3.2.2 Expression pattern under diverse hormones treatment | 第38-40页 |
| 3.2.3 Expression analysis in cotton after V.dahliae inoculation | 第40页 |
| 3.3 Silencing of GhLOX2 & GhLOX5 enhance the susceptibilty of cotton to Verticillium dahliae | 第40-45页 |
| 3.4 Silencing of GhLOX2 & GhLOX5 enhance the susceptibilty,of cotton to Botrytis cinerea | 第45-47页 |
| 3.5 Transformation and Regeneration of Cotton plants | 第47-50页 |
| 3.5.1 Selection and expression profile of positive plants | 第49-50页 |
| 4. Discussion | 第50-53页 |
| References | 第53-64页 |
| Acknowledgements | 第64页 |
