| Abstract | 第1-5页 |
| 中文摘要 | 第5-7页 |
| Abbreviations | 第7-12页 |
| Review | 第12-48页 |
| 1. Cell migration | 第12-20页 |
| ·The migration cycle | 第12-13页 |
| ·Actin-based protrusions | 第13-16页 |
| ·The polarization of the motile cell | 第16-20页 |
| 2. Border cell migration | 第20-28页 |
| ·The border cell model | 第20-21页 |
| ·The initiation of border cell migration | 第21-25页 |
| ·Guidance of border cells | 第25-26页 |
| ·The motility of border cell migration | 第26-27页 |
| ·A model for tumor metastasis | 第27-28页 |
| 3. Exocyst complex | 第28-38页 |
| ·The cellular secretory pathway | 第28-31页 |
| ·The function of the exocyst complex | 第31-33页 |
| ·The structure of the exocyst | 第33-35页 |
| ·Regulation of the exocyst | 第35-36页 |
| ·Exocyst function in cell migration | 第36-38页 |
| References | 第38-48页 |
| Chapter 1 The Drosophila sec3 gene is required for border cell migration | 第48-61页 |
| Introduction | 第49-50页 |
| Results | 第50-56页 |
| ·The screen and mapping | 第50-54页 |
| ·Loss of sec3 function causes border cell migration delay | 第54-55页 |
| ·Effects of altering Sec3 levels in a small clone of bordercells | 第55-56页 |
| Discussion | 第56-57页 |
| Materials and Methods | 第57-59页 |
| References | 第59-61页 |
| Chapter 2 Sec3 regulates vesicle transport of molecules essential for border cell migration | 第61-80页 |
| Introduction | 第62-64页 |
| Results | 第64-73页 |
| ·sec3 mutant border cell clusters fail to form lamellipodialprotrusion and can not display polarized localization ofRTKs | 第64-65页 |
| ·sec3 mutant border cells often accumulate adherensjunction proteins but not apical and lateral junction marker | 第65-67页 |
| ·The accumulated proteins are mostly localized in cytoplasm | 第67-70页 |
| ·Sec3 regulates recycling endosome transport | 第70-73页 |
| Discussion | 第73-75页 |
| Materials and Methods | 第75-76页 |
| References | 第76-80页 |
| Chapter 3 Sec3 works together with other exocyst proteins and the polarized vesicle transport is regulated by PVR and EGFR during border cell migration | 第80-94页 |
| Introduction | 第81-82页 |
| Results | 第82-87页 |
| ·sec3 functions together with other genes encoding exocyst components | 第82-83页 |
| ·Sec3 interacts with Sec5 | 第83-85页 |
| ·sec15 mutant shows the same phenotype as sec3 mutant | 第85-86页 |
| ·RTKs activity is required for vesicle trafficking mediated by exocyst in border cell migration | 第86-87页 |
| Discussion | 第87-90页 |
| Materials and Methods | 第90-91页 |
| Reference | 第91-94页 |
| Acknowledgements | 第94-96页 |
