| ABSTRACT | 第4-5页 |
| 摘要 | 第6-8页 |
| List of Contents | 第8-10页 |
| List of Figures | 第10-12页 |
| List of Tables | 第12-13页 |
| 1. Introduction | 第13-28页 |
| 1.1 The Principle Reasons for Studying the Spermatozoa Ion Channels | 第13-14页 |
| 1.2 Sperm Physiology | 第14-15页 |
| 1.3 Maturation of Epididymal sperm | 第15页 |
| 1.4 Sperm Capacitation | 第15-17页 |
| 1.5 Hyperactivation | 第17-19页 |
| 1.6 The acrosome reaction | 第19-20页 |
| 1.7 Calcium signaling in sperm | 第20-21页 |
| 1.8 Sperm Cells Ca~(2+) Channels | 第21-24页 |
| 1.9 Classification of Ion Channels | 第24页 |
| 1.10 CatSper form a Ca~(2+) -selective channel | 第24-28页 |
| 1.10.1 Biophysical properties of Catsper channel | 第25-26页 |
| 1.10.2 Biophysical characterization of CatSper currents(ICatSper) | 第26-28页 |
| 2. Potassium channels Ksper and its role in male fertility | 第28-42页 |
| 2.1 Potassium Channel of Sperm(KSper/Slo3) | 第28-29页 |
| 2.2 Biophysical properties of Slo3 | 第29-31页 |
| 2.3 Other Calcium(Cation)Channels | 第31-32页 |
| 2.4 Ca~(2+)-Activated Cl~- Channels(CaCCs) | 第32页 |
| 2.5 Na~+/H~+ exchangers | 第32-33页 |
| 2.6 CatSper and Slo3 as a pH_i dependent ion channels | 第33-36页 |
| 2.7 Pharmacological Compounds were used for characterization of Ca~(2+) channels in sperms | 第36-42页 |
| 3. Materials and Methods | 第42-50页 |
| 3.1 Protocol of Ca~(2+) Imaging | 第42-45页 |
| 3.2 Pharmacology | 第45-50页 |
| 4. Functional study of ksper and catsper channels in regulatingintracellular Ca~(2+) concentration and maintaining membrane potential ofsperm cells | 第50-82页 |
| 4.1 Introduction | 第50-51页 |
| 4.2 Materials and Methods | 第51-55页 |
| 4.3 Statistical Analysis | 第55-56页 |
| 4.4 Results | 第56-76页 |
| 4.5 Discussion | 第76-80页 |
| 4.6 Conclusion | 第80-82页 |
| Acknowledgement | 第82-83页 |
| References | 第83-101页 |
| Publication during my doctor degree | 第101-102页 |
| Abbreviations | 第102-103页 |
