| Abstract | 第5-6页 |
| CHAPTER 1:GENERAL INTRODUCTION | 第11-34页 |
| 1.1 High throughput screening | 第13-21页 |
| 1.1.1 Automation | 第13-15页 |
| 1.1.2 Miniaturization | 第15-20页 |
| 1.1.3 High sensitivity detection system | 第20页 |
| 1.1.4 Data management | 第20-21页 |
| 1.2 Cephalosporin C | 第21-24页 |
| 1.2.1 The structure of cephalosporin C | 第21-22页 |
| 1.2.2 The biosynthesis pathway of cephalosporin C | 第22-23页 |
| 1.2.3 Properties of cephalosporin C | 第23-24页 |
| 1.2.4 Cephalosporin C producing strains | 第24页 |
| 1.3 Mutants | 第24-28页 |
| 1.3.1 Physical mutation | 第24-26页 |
| 1.3.2 Chemical mutagenesis | 第26页 |
| 1.3.3 Compound mutation | 第26-27页 |
| 1.3.4 Atmospheric temperature plasma (ARTP) mutagenesis | 第27-28页 |
| 1.4 Genipin | 第28-32页 |
| 1.4.1 Genipin Information | 第28-30页 |
| 1.4.2 Natural Source | 第30页 |
| 1.4.3 Isolation of geniposide and genipin | 第30-31页 |
| 1.4.4 Genipin and Biomaterials | 第31-32页 |
| 1.5 Objects of this thesis | 第32-34页 |
| CHAPTER 2:MATERIALS AND METHODS | 第34-45页 |
| 2.1 Materials | 第34-37页 |
| 2.1.1 Microorganism | 第34页 |
| 2.1.2 Cultivation conditions | 第34-35页 |
| 2.1.2.1 Medium of Cephalosporium acremonium | 第34-35页 |
| 2.1.2.2 Medium of Alcaligenes faecalis(ATCC 8750) | 第35页 |
| 2.1.2.3 Medium of Brevibacterium flavum | 第35页 |
| 2.1.3 Equipment | 第35-36页 |
| 2.1.4 Laboratory reagent | 第36-37页 |
| 2.2 Methods | 第37-45页 |
| 2.2.1 Determination oxygen transfer coefficient(K_La) | 第37-38页 |
| 2.2.2 Determination of evaporation | 第38-39页 |
| 2.2.3 Determination of biomass | 第39页 |
| 2.2.4 Determination of cephalosporin C | 第39-41页 |
| 2.2.4.1 HPLC assay | 第39-40页 |
| 2.2.4.2 Turbidimetric assay | 第40-41页 |
| 2.2.5 L-Isoleucine and Genipin of methods | 第41-45页 |
| 2.2.5.1 Training methods | 第41页 |
| 2.2.5.2 Mutation | 第41-42页 |
| 2.2.5.3 High-through screening methods | 第42页 |
| 2.2.5.4 Broth pretreatment methods | 第42页 |
| 2.2.5.5 Determination methods | 第42页 |
| 2.2.5.6 Selection of high-throughput screening device | 第42页 |
| 2.2.5.7 L-Isoleicine establishment of hight-throughput screening methods | 第42-43页 |
| 2.2.5.8 Optimization of culture conditions | 第43-45页 |
| CHAPTER 3:High Throughput Optimization of Medium for Cephalosporin C Productivity | 第45-82页 |
| 3.1 Study oxygen transfer and evaporation of microtiter plates in micro-culture | 第45-53页 |
| 3.1.1 Study oxygen transfer of 24-well,48-well and 96-well microtiter plates | 第46-48页 |
| 3.1.2 Study evaporation of 24-well,48-well and 96-well microtiter plates | 第48-53页 |
| 3.2 Study applied capabilities of 24-well,48-well microtiter plate for cultivating Cephalosporium acremonium | 第53-60页 |
| 3.2.1 Effect of different fermentation medium volumes on production cephalosporin C and biomass in 24-well microtiter plates | 第54-56页 |
| 3.2.2 Effect of different fermentation medium volumes on production cephalosporin C and biomass in 48-well microtiter plates | 第56-57页 |
| 3.2.3 Comparison of cephalosporin C productivity between in 48-well microtiter plates and shake flasks cultivation | 第57-58页 |
| 3.2.4 Comparison of cephalosporin C productivity between UV assay and HPLC assay on shake flasks cultivation | 第58-60页 |
| 3.3 Investigation of the parallelity among wells in 24-well and 48-well microtiter plates | 第60-62页 |
| 3.3.1 Investigation of the parallelity among wells in 24-well microtiter plate | 第60-61页 |
| 3.3.2 Investigation of the parallelity among wells in 48-well microtiter plate | 第61-62页 |
| 3.4 Optimization of cultural conditions in microtiter plates | 第62-67页 |
| 3.4.1 Optimization of inoculum level(%) | 第62-64页 |
| 3.4.2 Optimization of inoculum age | 第64页 |
| 3.4.3 Optimization of cultural time | 第64-66页 |
| 3.4.4 Effect of different concentration of starch on cultural time of 24-well microtiter plates | 第66-67页 |
| 3.5 High throughpul optimizing nitrogen source for fermentation medium to culture Cephalosporium acremonium producing cephalosporin C | 第67-79页 |
| 3.5.1 Establishment of nitrogen source libraries | 第67-68页 |
| 3.5.2 High throughput optimization about nitrogen source for fermentation medium | 第68-69页 |
| 3.5.3 The results | 第69-79页 |
| 3.6 High throughput screening method for high productivity of cephalosporin C strains | 第79-82页 |
| 3.6.1 Description | 第79-81页 |
| 3.6.2 The results | 第81-82页 |
| CHAPTER 4:Preliminary Exploration of High-Throughput Screening for High-Yield L-Isoleucine Producing Strain | 第82-98页 |
| 4.1 Genipin with L-isoleucine optimal reaction conditions | 第82-85页 |
| 4.1.1 The reaction temperature | 第82-83页 |
| 4.1.2 Reaction time | 第83-85页 |
| 4.2 Establish Genipin with L-isoleucine reaction detection method | 第85-94页 |
| 4.2.1 Determine the detection waveleghth | 第85-88页 |
| 4.2.2 Standard curve | 第88-92页 |
| 4.2.3 Interference verification | 第92-94页 |
| 4.3 Parallet test plates | 第94-98页 |
| 4.3.1 Experimental strategies | 第94页 |
| 4.3.2 The experimental results | 第94-96页 |
| 4.3.3 Analysis and Discussion | 第96-98页 |
| CHAPTER 5:CONCLUSIONS AND PROSPECTS | 第98-101页 |
| 5.1 Conclusions | 第98-99页 |
| 5.2 Prospects | 第99-101页 |
| REFERENCE | 第101-109页 |
| ACKNOWLEDGEMENTS | 第109页 |
