| Abstract | 第6-7页 |
| 摘要 | 第8-11页 |
| Abbreviations | 第11-12页 |
| CHAPTER Ⅰ Introduction | 第12-35页 |
| 1.1 Importance of Ethylene | 第12-23页 |
| 1.2 Ethylene biosynthesis pathway | 第23-27页 |
| 1.3 Ethylene signaling Pathway | 第27-29页 |
| 1.4 Ethylene Responsive factor (ERF) | 第29-33页 |
| 1.5 The Research Prospect | 第33-35页 |
| CHAPTER Ⅱ Isolation and functional characterization of an Ethylene Response Factor(RhERF092) transcription factor in rose flower opening | 第35-75页 |
| 1. Introduction | 第35-37页 |
| 1.1 Research Approach | 第36页 |
| 1.2 Research contents | 第36-37页 |
| 2. Materials And Methods | 第37-45页 |
| 2.1 Methodologies | 第37-38页 |
| 2.2 Procedures | 第38-45页 |
| 3. RESULTS | 第45-72页 |
| 3.1 Selection of RhERF092 | 第45-46页 |
| 3.2 Expression Pattern for Five ERF Rose Unigenes | 第46-48页 |
| 3.3 Annotation of Ethylene Responsive Factor Genes (RhERFs) in Roses | 第48-49页 |
| 3.4 Cloning and sequence analysis of RhERF092 gene from Cut Rose flower | 第49-50页 |
| 3.5 Nucleotide and Amino Acid Sequences of RhERF092 | 第50-52页 |
| 3.6 Putative AP2-EREBP conserved Domain of RhERF092 | 第52-53页 |
| 3.7 Phylogentic Analysis of RhERF092 | 第53-55页 |
| 3.8 Expression profile of RhERF092 gene in response to ethylene in rose petals | 第55-57页 |
| 3.9 Subcellular localization of RhERF092 | 第57-58页 |
| 3.10 RhERF092 Silencing in Rose Flowers and Petals | 第58-61页 |
| 3.11 Overexpressin of RhERF092in transgenic Arabidopsis | 第61-64页 |
| 3.12 Root Length Analysis for Transgenic lines | 第64页 |
| 3.13 ACC treatment of RhERF092 transgene lines | 第64-66页 |
| 3.14 Abiotic stress and Hormonal response of RhERF092 transgenic lines | 第66-69页 |
| 3.15 Expression of genes related to cell proliferation and expansion in RhERF092-oxtransgenic plants | 第69-72页 |
| 4. DISCUSSION | 第72-75页 |
| CHAPTER Ⅲ Characterization of the RhACSl and RhACS2 promoter | 第75-91页 |
| 1. Introduction | 第75-77页 |
| 2. Material and Methods | 第77-80页 |
| 2.1 Construction of plant expression vectors and Arabidopsis transformation | 第77页 |
| 2.2 Hormone and abiotic stress treatments | 第77页 |
| 2.3 ABA, Mannitol and Drought stress treatments to transgenic Arabidopsis | 第77-78页 |
| 2.4 Relative quantitative RT-PCR | 第78页 |
| 2.5 Histochemical GUS staining | 第78页 |
| 2.6 Microscopic analysis | 第78-79页 |
| 2.7 GUS histochemical assay | 第79页 |
| 2.8 GUS activity assay | 第79页 |
| 2.9 Determination of soluble protein | 第79-80页 |
| 3. Results | 第80-88页 |
| 3.1 Analysis of the promoter activity of RhACS2 | 第80-81页 |
| 3.2 Response of the RhACS1 promoter to abiotic stresses and hormone | 第81-84页 |
| 3.3 Response of the RhACS2 promoter to abiotic stresses and hormone | 第84-88页 |
| 4. Discussion | 第88-91页 |
| CHAPTER Ⅳ CONCLUSION | 第91-92页 |
| ACKNOWKLEDGEMENT | 第92-93页 |
| References | 第93-104页 |
| Resume | 第104-105页 |
